In the study, it was aimed to determine the change in fruit quality characteristics during the shelf life by applying different doses of methyl jasmonate to tomato fruit harvested in 2 different maturity periods which green and pink maturity level. Post-harvest tomato fruit were treated with three different doses of methyl jasmonate concentration (control (water), 0.5 and 1.0 mmol L⁻¹) at room conditions. Fruit weight losses, respiration rate, fruit quality characteristics, fruit firmness, color values and phenolic substance contents were determined on the $10^{th} and 20^{th}$ days of the treated fruit. At the end of the $20^{th}$ day, while the weight loss of tomato fruit harvested in the green stage period varied between 2.99-6.97%, these values were determined between 2.49-6.58%in fruit harvested at pink maturity. While the weight losses were highest at 0.5 mmol L⁻¹ MeJA dose in the green stage, the highest losses were observed at the 1.0 mmol L⁻¹ MeJA dose in the fruit harvested during the pink stage. At the end of the 20th day, the vitamin C values varied between 89.50-153.0 mg 100 g⁻¹ in the fruit harvested at the end of the 20th day, while these values changed between 165.50-210.00 mg 100 g⁻¹ in the pink ripening period. As a result of the study, it was determined that MeJA treatments had significant effects on some shelf life properties of tomato fruits kept at room conditions.