Nowadays, materials such as vermicompost obtained by earthworm activity are used in the production of vegetables and vegetable seedlings to improve the quality of the growing medium. In addition, due to reasons such as increasing environmental awareness, high input costs, recycling of wastes, and the desire to reduce soil and water pollution, there is an increasing interest in investigating the usability of different resources for the mentioned purpose. Terrestrial isopods such as Porcellio laevis, essential members of the soil macrofauna, are transformative species that play an important role in the decomposition of livestock and agriculture wastes. In the study, vermicompost and isopod fertilizer were added to the lettuce growing medium (soil) at different rates (1%, 5%, 10%, 20%), and the changes in some chemical properties of the media before and after growing (end of harvest) were investigated. The addition of vermicompost and isopod fertilizers led to an increase in macro and micronutrient levels. In both fertilizers, an increase in the amount of salt was observed with the increase in dose, but it was determined that this was not at a level that would cause salinity. According to the result of the end-harvest analysis, there was a significant decrease in the manganese level and the amount of zinc in the growing media increased linearly by the increasing of isopod fertilizer doses. In addition, it was determined that the post-harvest nutrient levels of the media were adequate to reuse for seedling and plant growing.