Objective: Heterocycles with a pyrimidopyrimidine scaffold have been the focus of interest of researchers in the field of medicinal chemistry as they have a wide range of biological properties. In the current study antioxidant capacity and antibacterial activity of a series of new pyrimido[1,2-a]pyrimidines were investigatedMaterial and Method: All these novel compounds were screened for their in vitro antioxidant effectiveness using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging test and the antibacterial activity was determined using the broth microdilution method according to The European Committee on Antimicrobial Susceptibility Testing standards (EUCAST) as a minimal inhibition concentration (MIK) against some Gram- positive and negative ATCC strains.Result and Discussion: The overall range of DPPH free radical scavenging activity was found to be 3.86-6.90% at 0.1mM and 6.28–16.59% at 1mM for the novel pyrimidopyrimidine derivates. Since the free radical scavenging activities of all the compounds were below 20%, it was considered that the weak activity of compounds might be due to the absence of an enolizable amide group in the pyrimidine ring. In addition to that, some of the selected compounds showed weak antioxidant activity. MIC values of all compounds were found as > 100 µg/ml against Staphylococcus aureus ATCC 29213, methicillin resistant Staphylococcus aureus ATCC 43300, Enterococcus faecalis 29212, Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 13883, Pseudomonas aeruginosa ATCC 27853. The data obtained from present study require the synthesis of derivatives with various substituents in different positions of the pyrimidine ring in order to be able to evaluate and interpret more comprehensively in future studies.