TY  - JOUR
TI  - EVALUATION OF ROUNDUP® TOXICITY IN HUMAN LUNG CELLS
AB  - Objective: In this study, toxic effects of Roundup, one of the most common glyphosate-based herbicides (GBHs), were assessed on human bronchial epithelial cells (BEAS-2B).Material and Method: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and neutral red uptake assays were implemented for evaluation of cell viability at 24 and 48 h. Apoptosis detection was made by Muse analyzer while Hoechst staining was employed to detect apoptotic nuclear changes. In addition, dichlorofluorescein diacetate assay was used for the assessment of reactive oxygen species (ROS) formation.Result and Discussion: Similar half maximal inhibitory concentrations were obtained from cytotoxicity assays. Results showed that significant reduction in the viability of BEAS-2B cells started to occur from 200 µM at 24 h and 50 µM at 48 h treatment times. Roundup treatments were found to cause apoptosis in a dose-dependent manner in both time points and apoptosis increased with increasing exposure time. Moreover, it was observed that cellular ROS formation was induced following Roundup exposure. These findings suggest that GBHs can stimulate ROS production, as well as apoptosis on healthy human lung cells which is important considering inhalation is one of the primary exposure routes to this group of chemicals.  
AU  - BAKIR, ELÇIN
AU  - gurbay, Aylin
AU  - Hamurcu, Zuhal
AU  - ÖKÇESİZ HACISEYİTOĞLU, AYSUN
AU  - EKEN, AYSE
AU  - ÜNLÜ ENDİRLİK, Burcu
DO  - 10.33483/jfpau.1200312
PY  - 2023
JO  - Ankara Üniversitesi Eczacılık Fakültesi Dergisi
VL  - 47
IS  - 1
SN  - 1015-3918
SP  - 260
EP  - 268
DB  - TRDizin
UR  - http://search/yayin/detay/1155484
ER  -