Our country is located on the origin geography of the vine spreading areas in the world, and the best proof of this is the rich viticulture Vitis vinifera ssp. sativa and wild vine Vitis vinifera ssp. silvestris has genotype diversity. In this study, it was carried out between 2017 and 2018 in order to comprehensively examine the local types/varieties cultivated in Eskişehir and to determine the ampelographic characteristics of the determined grape varieties in accordance with international norms. In the research, 52 grape varieties determined in different districts of Eskişehir were evaluated in terms of ampelographic characteristics with 79 characters including leaves, shoots, flowers, clusters, berry and seeds. Differences were determined between the cultivars in terms of the examined characteristics. The grape cultivars/types identified in the study contributed to the scientific literature related to our current gene resources.