On chip microfluidic separation of cyclotides

Cyclotides as a cyclic peptide produced by different groups of plants have been a very attractive field of research due to their exceptional properties in biological activities and drug design applications. The importance of cyclotides as new biological activities from nature caused to attract researchers to develop new separation systems. Recent growth and development on chip-based technology for separation and bioassay especially for anticancer having sparklingly advantages comparison with common traditional methods. In this study, the microfluidic separation of Vigno 1-5 cyclotides extracted from Viola ignobilis by using polar and nonpolar forces as a liquid-liquid interaction was investigated through modified microfluidic chips and then the results were compared with a traditional counterpart technique of high-performance liquid chromatography (HPLC). The traditional process of separating cyclotides from plants is a costly and time-consuming procedure. The scientific novelty of this study is to accelerate the separation of cyclotides using modified microfluidic chips with low cost and high efficiency. The results revealed that a novel and simple microfluidic chip concept is an effective approach for separating the Vigno groups in the violet extract. We believe that the concept could potentially be utilized for further drug development process especially for anticancer studies by coupling bioassay chips as online procedures via reducing in time and cost compared with traditional offline methods.

Anahtar Kelime: Microfluidic separation cyclotide Viola ignobilis Vigno 5 liquid-liquid extraction

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Didarian R, EBRAHIMI A, Ghorbanpoor H, Bagheroghli H, Doğan Güzel F, faradpour m, Lotfibakhshaiesh N, Hashempour H, Avci H (2023). On chip microfluidic separation of cyclotides. , 253 - 262. 10.55730/1300-0527.3534
Chicago	Didarian Reza,EBRAHIMI Aliakbar,Ghorbanpoor Hamed,Bagheroghli Hessam,Doğan Güzel Fatma,faradpour mohsen,Lotfibakhshaiesh Nasrin,Hashempour Hossein,Avci Huseyin On chip microfluidic separation of cyclotides. (2023): 253 - 262. 10.55730/1300-0527.3534
MLA	Didarian Reza,EBRAHIMI Aliakbar,Ghorbanpoor Hamed,Bagheroghli Hessam,Doğan Güzel Fatma,faradpour mohsen,Lotfibakhshaiesh Nasrin,Hashempour Hossein,Avci Huseyin On chip microfluidic separation of cyclotides. , 2023, ss.253 - 262. 10.55730/1300-0527.3534
AMA	Didarian R,EBRAHIMI A,Ghorbanpoor H,Bagheroghli H,Doğan Güzel F,faradpour m,Lotfibakhshaiesh N,Hashempour H,Avci H On chip microfluidic separation of cyclotides. . 2023; 253 - 262. 10.55730/1300-0527.3534
Vancouver	Didarian R,EBRAHIMI A,Ghorbanpoor H,Bagheroghli H,Doğan Güzel F,faradpour m,Lotfibakhshaiesh N,Hashempour H,Avci H On chip microfluidic separation of cyclotides. . 2023; 253 - 262. 10.55730/1300-0527.3534
IEEE	Didarian R,EBRAHIMI A,Ghorbanpoor H,Bagheroghli H,Doğan Güzel F,faradpour m,Lotfibakhshaiesh N,Hashempour H,Avci H "On chip microfluidic separation of cyclotides." , ss.253 - 262, 2023. 10.55730/1300-0527.3534
ISNAD	Didarian, Reza vd. "On chip microfluidic separation of cyclotides". (2023), 253-262. https://doi.org/10.55730/1300-0527.3534

APA	Didarian R, EBRAHIMI A, Ghorbanpoor H, Bagheroghli H, Doğan Güzel F, faradpour m, Lotfibakhshaiesh N, Hashempour H, Avci H (2023). On chip microfluidic separation of cyclotides. Turkish Journal of Chemistry, 47(1), 253 - 262. 10.55730/1300-0527.3534
Chicago	Didarian Reza,EBRAHIMI Aliakbar,Ghorbanpoor Hamed,Bagheroghli Hessam,Doğan Güzel Fatma,faradpour mohsen,Lotfibakhshaiesh Nasrin,Hashempour Hossein,Avci Huseyin On chip microfluidic separation of cyclotides. Turkish Journal of Chemistry 47, no.1 (2023): 253 - 262. 10.55730/1300-0527.3534
MLA	Didarian Reza,EBRAHIMI Aliakbar,Ghorbanpoor Hamed,Bagheroghli Hessam,Doğan Güzel Fatma,faradpour mohsen,Lotfibakhshaiesh Nasrin,Hashempour Hossein,Avci Huseyin On chip microfluidic separation of cyclotides. Turkish Journal of Chemistry, vol.47, no.1, 2023, ss.253 - 262. 10.55730/1300-0527.3534
AMA	Didarian R,EBRAHIMI A,Ghorbanpoor H,Bagheroghli H,Doğan Güzel F,faradpour m,Lotfibakhshaiesh N,Hashempour H,Avci H On chip microfluidic separation of cyclotides. Turkish Journal of Chemistry. 2023; 47(1): 253 - 262. 10.55730/1300-0527.3534
Vancouver	Didarian R,EBRAHIMI A,Ghorbanpoor H,Bagheroghli H,Doğan Güzel F,faradpour m,Lotfibakhshaiesh N,Hashempour H,Avci H On chip microfluidic separation of cyclotides. Turkish Journal of Chemistry. 2023; 47(1): 253 - 262. 10.55730/1300-0527.3534
IEEE	Didarian R,EBRAHIMI A,Ghorbanpoor H,Bagheroghli H,Doğan Güzel F,faradpour m,Lotfibakhshaiesh N,Hashempour H,Avci H "On chip microfluidic separation of cyclotides." Turkish Journal of Chemistry, 47, ss.253 - 262, 2023. 10.55730/1300-0527.3534
ISNAD	Didarian, Reza vd. "On chip microfluidic separation of cyclotides". Turkish Journal of Chemistry 47/1 (2023), 253-262. https://doi.org/10.55730/1300-0527.3534