Evaluation of Phytochemical Contents and Biological Activities of Salvia officinalis and Salvia triloba Grown with Organic Farming
Yıl: 2023 Cilt: 48 Sayı: 1 Sayfa Aralığı: 125 - 138 Metin Dili: İngilizce DOI: 10.55262/fabadeczacilik.1175781 İndeks Tarihi: 28-03-2023
Evaluation of Phytochemical Contents and Biological Activities of Salvia officinalis and Salvia triloba Grown with Organic Farming
Öz: Salvia officinalis L., known as medicinal sage, and Salvia triloba L., known as Anatolian sage, belong to the Lamiaceae family and are species that usually grow in the Mediterranean region. In this study, it was aimed to evaluate the in vitro antidiabetic, antiobesity and antioxidant potentials of the extracts prepared by infusion technique from S. officinalis and S. triloba has grown by organic farming methods. In addition, the effects of the extracts on the pancreatic cholesterol esterase enzyme were also investigated.The Reverse Phase- High Performance Liquid Chromatography (HPLC) technique was used to analyze the phytochemical contents of the extracts. At a concentration of 2 mg/mL, S. officinalis inhibited 64.69% ± 0.23, S. triloba 47.78 ± 2.11% on the α-glucosidase enzyme. Only S. triloba had an inhibitory effect on α-amylase and pancreatic lipase enzyme. On the pancreatic cholesterol esterase enzyme, inhibition values of S. triloba extract at all tested concentrations was found higher than S. officinalis extract. It was observed that the S. officinalis extract had the highest reducing power potential. The metal chelating capacity of both extracts at a concentration of 2 mg/mL was calculated as 100%. It was concluded that the ABTS radical scavenging activity of the extracts increased in a dose-dependently manner. The amounts of rosmarinic acid and hesperidin were found higher in S. officinalis extract than in S. triloba extract by Reverse Phase-HPLC technique. The presence of hesperidin in S. triloba was detected for the first time in this study. These findings considering it was concluded that activity- guided isolation and in vivo activity studies should be performed because these two species grown the organic farming methods have potent α-glucosidase enzyme inhibitory and antioxidant effects.
Anahtar Kelime: Organik Tarım ile Yetiştirilen Salvia officinalis ve Salvia triloba’nın Fitokimyasal İçeriklerinin ve Biyolojik Aktivitelerinin Değerlendirilmesi
Öz: Tıbbi adaçayı olarak bilinen Salvia officinalis L. ve Anadolu adaçayı olarak bilinen Salvia triloba L., Lamiaceae familyasına ait olup, genellikle Akdeniz bölgesinde yetişen türlerdir. Bu çalışmada organik tarım yöntemleri ile yetiştirilmiş S. officinalis ve S. triloba’dan infüzyon tekniği ile hazırlanan ekstrelerinin in vitro antidiyabetik, antiobezite ve antioksidan potansiyellerinin değerlendirilmesi amaçlanmıştır. Bunların yanı sıra ekstrelerin pankreatik kolesterol esteraz enzimi üzerindeki etkileri de incelenmiştir. Ekstrelerin fitokimyasal içeriklerini analiz etmek için Ters Faz-Yüksek Performanslı Sıvı Kromatografisi (YPSK) tekniği kullanılmıştır. α-Glukozidaz enzimi üzerinde 2 mg/mL konsantrasyonda S. officinalis %64.69 ± 0.23, S. triloba ise %47.78 ± 2.11 inhibisyona neden olmuştur. α-Amilaz ve pankreatik lipaz enzimi üzerinde sadece S.triloba inhibitör etki oluşturmuştur. Pankreatik kolesterol esteraz enzimi üzerinde ise S. triloba ekstresinin test edilen tüm konsantrasyonlardaki inhibisyon değerlerinin S. officinalis ekstresinden daha yüksek olduğu tespit edilmiştir. S. officinalis ekstresinin en yüksek indirgeme gücü potansiyeline sahip olduğu gözlemlendi. Her iki ekstrenin 2 mg/ mL konsantrasyonda metal bağlama kapasitesi %100 olarak hesaplanmıştır. Ekstrelerin ABTS radikal süpürücü aktivitesinin ise doz bağımlı olarak arttığı sonucuna varılmıştır.Ters Faz-YPSK tekniği ile S. officinalis ekstresinde rosmarinik asit ve hesperidin miktarları S. triloba ekstresine göre daha yüksek bulunmuştur. S. triloba’da hesperidinin varlığı ilk kez bu çalışmada tespit edilmiştir. Tüm bu bulgular göz önüne alındığında, organik tarım yöntemiyle yetiştirilen bu iki türün güçlü α-glukozidaz enzim inhibitör ve antioksidan etkilere sahip olmasından dolayı üzerlerinde aktivite yönlendirmeli izolasyon ve in vivo aktivite çalışmalarının yapılması gerektiği sonucuna varılmıştır.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | ÖZÜPEK B, Pekacar S, Deliorman Orhan D (2023). Evaluation of Phytochemical Contents and Biological Activities of Salvia officinalis and Salvia triloba Grown with Organic Farming. , 125 - 138. 10.55262/fabadeczacilik.1175781 |
| Chicago | ÖZÜPEK BURÇİN,Pekacar Sultan,Deliorman Orhan Didem Evaluation of Phytochemical Contents and Biological Activities of Salvia officinalis and Salvia triloba Grown with Organic Farming. (2023): 125 - 138. 10.55262/fabadeczacilik.1175781 |
| MLA | ÖZÜPEK BURÇİN,Pekacar Sultan,Deliorman Orhan Didem Evaluation of Phytochemical Contents and Biological Activities of Salvia officinalis and Salvia triloba Grown with Organic Farming. , 2023, ss.125 - 138. 10.55262/fabadeczacilik.1175781 |
| AMA | ÖZÜPEK B,Pekacar S,Deliorman Orhan D Evaluation of Phytochemical Contents and Biological Activities of Salvia officinalis and Salvia triloba Grown with Organic Farming. . 2023; 125 - 138. 10.55262/fabadeczacilik.1175781 |
| Vancouver | ÖZÜPEK B,Pekacar S,Deliorman Orhan D Evaluation of Phytochemical Contents and Biological Activities of Salvia officinalis and Salvia triloba Grown with Organic Farming. . 2023; 125 - 138. 10.55262/fabadeczacilik.1175781 |
| IEEE | ÖZÜPEK B,Pekacar S,Deliorman Orhan D "Evaluation of Phytochemical Contents and Biological Activities of Salvia officinalis and Salvia triloba Grown with Organic Farming." , ss.125 - 138, 2023. 10.55262/fabadeczacilik.1175781 |
| ISNAD | ÖZÜPEK, BURÇİN vd. "Evaluation of Phytochemical Contents and Biological Activities of Salvia officinalis and Salvia triloba Grown with Organic Farming". (2023), 125-138. https://doi.org/10.55262/fabadeczacilik.1175781 |
| APA | ÖZÜPEK B, Pekacar S, Deliorman Orhan D (2023). Evaluation of Phytochemical Contents and Biological Activities of Salvia officinalis and Salvia triloba Grown with Organic Farming. FABAD Journal of Pharmaceutical Sciences, 48(1), 125 - 138. 10.55262/fabadeczacilik.1175781 |
| Chicago | ÖZÜPEK BURÇİN,Pekacar Sultan,Deliorman Orhan Didem Evaluation of Phytochemical Contents and Biological Activities of Salvia officinalis and Salvia triloba Grown with Organic Farming. FABAD Journal of Pharmaceutical Sciences 48, no.1 (2023): 125 - 138. 10.55262/fabadeczacilik.1175781 |
| MLA | ÖZÜPEK BURÇİN,Pekacar Sultan,Deliorman Orhan Didem Evaluation of Phytochemical Contents and Biological Activities of Salvia officinalis and Salvia triloba Grown with Organic Farming. FABAD Journal of Pharmaceutical Sciences, vol.48, no.1, 2023, ss.125 - 138. 10.55262/fabadeczacilik.1175781 |
| AMA | ÖZÜPEK B,Pekacar S,Deliorman Orhan D Evaluation of Phytochemical Contents and Biological Activities of Salvia officinalis and Salvia triloba Grown with Organic Farming. FABAD Journal of Pharmaceutical Sciences. 2023; 48(1): 125 - 138. 10.55262/fabadeczacilik.1175781 |
| Vancouver | ÖZÜPEK B,Pekacar S,Deliorman Orhan D Evaluation of Phytochemical Contents and Biological Activities of Salvia officinalis and Salvia triloba Grown with Organic Farming. FABAD Journal of Pharmaceutical Sciences. 2023; 48(1): 125 - 138. 10.55262/fabadeczacilik.1175781 |
| IEEE | ÖZÜPEK B,Pekacar S,Deliorman Orhan D "Evaluation of Phytochemical Contents and Biological Activities of Salvia officinalis and Salvia triloba Grown with Organic Farming." FABAD Journal of Pharmaceutical Sciences, 48, ss.125 - 138, 2023. 10.55262/fabadeczacilik.1175781 |
| ISNAD | ÖZÜPEK, BURÇİN vd. "Evaluation of Phytochemical Contents and Biological Activities of Salvia officinalis and Salvia triloba Grown with Organic Farming". FABAD Journal of Pharmaceutical Sciences 48/1 (2023), 125-138. https://doi.org/10.55262/fabadeczacilik.1175781 |
