Investigation of Dimethoate Toxicity in Rat Brain and Protective Effect of Laurocerasus officinalis Roem. Fruit Extract Against Oxidative Stress, DNA Damage, and Apoptosis
Yıl: 2023 Cilt: 48 Sayı: 1 Sayfa Aralığı: 139 - 150 Metin Dili: İngilizce DOI: 10.55262/fabadeczacilik.1214152 İndeks Tarihi: 28-03-2023
Investigation of Dimethoate Toxicity in Rat Brain and Protective Effect of Laurocerasus officinalis Roem. Fruit Extract Against Oxidative Stress, DNA Damage, and Apoptosis
Öz: Dimethoate is an organophosphate insecticide that is used globally on a wide scale in agriculture. It was associated with numerous negative health effects in many studies. The brain is one of the target organs for dimethoate exposure. The present study aimed to evaluate the sub- chronic (60 days) toxicity of dimethoate (7 mg/kg body weight) by investigating its oxidative stress, DNA damage, apoptosis-inducing effects, and histopathological changes in brain tissue of rats. We also aimed to analyze the protective effects of Laurocerasus officinalis Roem. (cherry laurel) fruit extract. To evaluate oxidative stress, malondialdehyde (MDA) levels, as well as superoxide dismutase (Cu-Zn SOD), glutathione peroxidase (GPx), and catalase (CAT) antioxidant enzymes activities, were calculated. Experimental results demonstrated that dimethoate treatment increased MDA and decreased Cu-Zn SOD, GPx, and CAT enzyme activities, suggesting its potency as an oxidative stress inducer in rat brain tissues. Furthermore, comet and TUNEL assay results showed that dimethoate stimulated DNA damage and apoptosis. Administration of cherry laurel extracts protected against dimethoate-induced oxidative stress, DNA damage, and apoptosis. The findings of the current study are important in terms of demonstrating the beneficial effects of L. officinalis extract against dimethoate toxicity in the brain, considering the sensitivity of this organ to oxidative stress and extensive usage of dimethoate.
Anahtar Kelime: Dimetoat Toksisitesinin Sıçan Beyninde Araştırılması ve Laurocerasus officinalis Roem. Meyve Ekstresinin Oksidatif Stres, DNA Hasarı ve Apoptoza Karşı Koruyucu Etkisi
Öz: Dimetoat dünyada tarımda yaygın olarak kullanılan organofosfatlı bir insektisittir. Bir çok çalışmada dimetoatın negatif sağlık etkileriyle ilişkili olduğu belirtilmiştir. Beyin, dimetoat maruziyeti için başlıca hedef organlarından birisidir. Bu çalışmadaki amacımız dimetoatın (7 mg/kg vücut ağırlığı) sub-kronik (60 günlük) toksisitesini, sıçan beyin dokuları üzerindeki oksidatif stres, DNA hasarı ve apoptoz indükleyici etkilerini ve histopatolojik değişiklikleri araştırarak değerlendirmektir. Ayrıca Laurocerasus officinalis Roem. (taflan) meyve ekstresinin koruyucu etkilerinin incelenmesi hedeflenmektedir. Oksidatif stres değerlendirmesi için malondialdehit düzeyleri (MDA) ile birlikte süperoksit dismutaz (Cu-Zn SOD), glutatyon peroksidaz (GPx) ve katalaz (CAT) antioksidan enzim aktiviteleri ölçülmüştür. Deney sonuçları dimetoat muamelesinin MDA’yı arttırdığını ve Cu- Zn SOD, GPx ve CAT enzim aktivitelerini azalttığını göstererek, sıçan beyin dokularında etkin bir oksidatif stres indükleyici olabileceğini ortaya koymaktadır. Ayrıca comet ve TUNEL deney sonuçları da dimetoatın DNA hasarı ve apoptozu arttırdığını göstermiştir. Taflan ekstresi verilmesi, dimetoat tarafından indüklenen oksidatif stres, DNA hasarı ve apoptoza karşı koruyucu etki sağlamıştır. Bu çalışmada elde edilen bulgular, beynin oksidatif strese duyarlılığı ve dimetoatın yaygın kullanımı göz önüne alındığında, L. officinalis ekstresinin dimetoat toksisitesine karşı beyinde faydalı etkilerini göstermesi açısından önem taşımaktadır.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | ÜNLÜ ENDİRLİK B, BAKIR E, Yay A, CANTÜRK F, Baldemir A, EKEN A (2023). Investigation of Dimethoate Toxicity in Rat Brain and Protective Effect of Laurocerasus officinalis Roem. Fruit Extract Against Oxidative Stress, DNA Damage, and Apoptosis. , 139 - 150. 10.55262/fabadeczacilik.1214152 |
| Chicago | ÜNLÜ ENDİRLİK Burcu,BAKIR ELÇIN,Yay Arzu Hanım,CANTÜRK Fazile,Baldemir Ayşe,EKEN AYSE Investigation of Dimethoate Toxicity in Rat Brain and Protective Effect of Laurocerasus officinalis Roem. Fruit Extract Against Oxidative Stress, DNA Damage, and Apoptosis. (2023): 139 - 150. 10.55262/fabadeczacilik.1214152 |
| MLA | ÜNLÜ ENDİRLİK Burcu,BAKIR ELÇIN,Yay Arzu Hanım,CANTÜRK Fazile,Baldemir Ayşe,EKEN AYSE Investigation of Dimethoate Toxicity in Rat Brain and Protective Effect of Laurocerasus officinalis Roem. Fruit Extract Against Oxidative Stress, DNA Damage, and Apoptosis. , 2023, ss.139 - 150. 10.55262/fabadeczacilik.1214152 |
| AMA | ÜNLÜ ENDİRLİK B,BAKIR E,Yay A,CANTÜRK F,Baldemir A,EKEN A Investigation of Dimethoate Toxicity in Rat Brain and Protective Effect of Laurocerasus officinalis Roem. Fruit Extract Against Oxidative Stress, DNA Damage, and Apoptosis. . 2023; 139 - 150. 10.55262/fabadeczacilik.1214152 |
| Vancouver | ÜNLÜ ENDİRLİK B,BAKIR E,Yay A,CANTÜRK F,Baldemir A,EKEN A Investigation of Dimethoate Toxicity in Rat Brain and Protective Effect of Laurocerasus officinalis Roem. Fruit Extract Against Oxidative Stress, DNA Damage, and Apoptosis. . 2023; 139 - 150. 10.55262/fabadeczacilik.1214152 |
| IEEE | ÜNLÜ ENDİRLİK B,BAKIR E,Yay A,CANTÜRK F,Baldemir A,EKEN A "Investigation of Dimethoate Toxicity in Rat Brain and Protective Effect of Laurocerasus officinalis Roem. Fruit Extract Against Oxidative Stress, DNA Damage, and Apoptosis." , ss.139 - 150, 2023. 10.55262/fabadeczacilik.1214152 |
| ISNAD | ÜNLÜ ENDİRLİK, Burcu vd. "Investigation of Dimethoate Toxicity in Rat Brain and Protective Effect of Laurocerasus officinalis Roem. Fruit Extract Against Oxidative Stress, DNA Damage, and Apoptosis". (2023), 139-150. https://doi.org/10.55262/fabadeczacilik.1214152 |
| APA | ÜNLÜ ENDİRLİK B, BAKIR E, Yay A, CANTÜRK F, Baldemir A, EKEN A (2023). Investigation of Dimethoate Toxicity in Rat Brain and Protective Effect of Laurocerasus officinalis Roem. Fruit Extract Against Oxidative Stress, DNA Damage, and Apoptosis. FABAD Journal of Pharmaceutical Sciences, 48(1), 139 - 150. 10.55262/fabadeczacilik.1214152 |
| Chicago | ÜNLÜ ENDİRLİK Burcu,BAKIR ELÇIN,Yay Arzu Hanım,CANTÜRK Fazile,Baldemir Ayşe,EKEN AYSE Investigation of Dimethoate Toxicity in Rat Brain and Protective Effect of Laurocerasus officinalis Roem. Fruit Extract Against Oxidative Stress, DNA Damage, and Apoptosis. FABAD Journal of Pharmaceutical Sciences 48, no.1 (2023): 139 - 150. 10.55262/fabadeczacilik.1214152 |
| MLA | ÜNLÜ ENDİRLİK Burcu,BAKIR ELÇIN,Yay Arzu Hanım,CANTÜRK Fazile,Baldemir Ayşe,EKEN AYSE Investigation of Dimethoate Toxicity in Rat Brain and Protective Effect of Laurocerasus officinalis Roem. Fruit Extract Against Oxidative Stress, DNA Damage, and Apoptosis. FABAD Journal of Pharmaceutical Sciences, vol.48, no.1, 2023, ss.139 - 150. 10.55262/fabadeczacilik.1214152 |
| AMA | ÜNLÜ ENDİRLİK B,BAKIR E,Yay A,CANTÜRK F,Baldemir A,EKEN A Investigation of Dimethoate Toxicity in Rat Brain and Protective Effect of Laurocerasus officinalis Roem. Fruit Extract Against Oxidative Stress, DNA Damage, and Apoptosis. FABAD Journal of Pharmaceutical Sciences. 2023; 48(1): 139 - 150. 10.55262/fabadeczacilik.1214152 |
| Vancouver | ÜNLÜ ENDİRLİK B,BAKIR E,Yay A,CANTÜRK F,Baldemir A,EKEN A Investigation of Dimethoate Toxicity in Rat Brain and Protective Effect of Laurocerasus officinalis Roem. Fruit Extract Against Oxidative Stress, DNA Damage, and Apoptosis. FABAD Journal of Pharmaceutical Sciences. 2023; 48(1): 139 - 150. 10.55262/fabadeczacilik.1214152 |
| IEEE | ÜNLÜ ENDİRLİK B,BAKIR E,Yay A,CANTÜRK F,Baldemir A,EKEN A "Investigation of Dimethoate Toxicity in Rat Brain and Protective Effect of Laurocerasus officinalis Roem. Fruit Extract Against Oxidative Stress, DNA Damage, and Apoptosis." FABAD Journal of Pharmaceutical Sciences, 48, ss.139 - 150, 2023. 10.55262/fabadeczacilik.1214152 |
| ISNAD | ÜNLÜ ENDİRLİK, Burcu vd. "Investigation of Dimethoate Toxicity in Rat Brain and Protective Effect of Laurocerasus officinalis Roem. Fruit Extract Against Oxidative Stress, DNA Damage, and Apoptosis". FABAD Journal of Pharmaceutical Sciences 48/1 (2023), 139-150. https://doi.org/10.55262/fabadeczacilik.1214152 |
