Light-Mediated Biosynthesis of Phenylpropanoid Metabolites in Cell Suspension Cultures of Turkish Yarrow (Achillea gypsicola Hub. Mor.)
Yıl: 2022 Cilt: 25 Sayı: 6 Sayfa Aralığı: 1234 - 1242 Metin Dili: İngilizce DOI: 10.18016/ksutarimdoga.vi.926160 İndeks Tarihi: 20-03-2023
Light-Mediated Biosynthesis of Phenylpropanoid Metabolites in Cell Suspension Cultures of Turkish Yarrow (Achillea gypsicola Hub. Mor.)
Öz: Turkish yarrow (Achillea gypsicola Hub. Mor.) contains medicinally valuable bioactive metabolites. Light plays a key role in many physiological events in plants such as the accumulation of phenylpropanoid metabolites, particularly photosynthesis. The aim of this study was to determine the effects of light on the biosynthesis of phenylpropanoid metabolites and biomass growth in Turkish yarrow cell suspension cultures. In vitro plantlets obtained from seeds were used as explant sources and cell cultures of A. gypsicola were established using stem segment-derived callus tissues. Then, 8-day-old cell cultures kept in a B5 medium supplemented with 0.5 mg/L BA (benzylaminopurine)+0.5 mg/L NAA (naphthalene acetic acid) were incubated in light and dark environments. Cells were harvested five times (1st, 2nd, 3rd, 6th, and 12th day) in total. The amount of camphor was determined by gas chromatography-mass spectrometry (GC–MS). Other parameters were calculated using the spectrophotometric method. The total phenolic amount increased depending on the sampling time in dark and light conditions. Phenolic accumulation was increased by 29.25% compared to the initial culture. Also, light application increased the amount of camphor 1.12 times, the amount of flavanol 4.9 times, and the amount of flavonol 0.5 times. These results clearly demonstrate the effect of light on cell growth and phenylpropanoid metabolite accumulation in A. gypsicola cell cultures.
Anahtar Kelime: Türk Civanperçemi (Achillea gypsicola Hub. Mor.) Hücre Süspansiyon Kültürlerinde Fenilpropanoid Metabolitlerin Işık Aracılığıyla Biosentezi
Öz: Türk civanperçemi (Achillea gypsicola Hub. Mor.) tıbbi açıdan değerli metabolitler içerir. Işık, bitkilerde başta fotosentez olmak üzere fenipropanoid metabolitlerin birikimi gibi birçok fizyolojik olayda kilit bir rol oynar. Bu çalışmanın amacı, ışığın Türk civanperçemi hücre süspansiyon kültüründe fenilpropanoid metabolitlerin biyosentezi ve biyokütle artışı üzerindeki etkilerini belirlemektir. Tohumlardan elde edilen in vitro bitkicikler eksplant kaynağı olarak kullanılmış ve gövde segmentinden türetilen kallus dokularından hücre kültürleri oluşturulmuştur. Daha sonra, 0.5 mg/L BA (benzilaminopurin)+0.5 mg/L NAA (naftalen asetik asit) ile desteklenmiş B5 ortamında muhafaza edien 8 günlük hücre kültürleri, aydınlık ve karanlık ortamlarda inkübe edilmiştir. Toplamda beş kez (1, 2, 3, 6 ve 12. gün) hücre hasadı yapılmıştır. Kamfor miktarı gas chromatography-mass spectrometry (GC-MS) ile belirlenmiştir. Diğer parametreler spektrofotometrik yöntem kullanılarak hesaplanmıştır. Toplam fenolik miktarı karanlık ve aydınlık koşullarda örnek alma zamanına bağlı olarak artış göstermiştir. Fenolik madde birikimi başlangıç kültürüne göre %29.25 artmıştır. Işık uygulaması kamfor miktarını 1.12 kat, flavanol miktarını 4.9 kat ve flavonol miktarını 0.5 kat artırmıştır. Bu sonuçlar bize A. gypsicola hücre kültürlerinde ışığın hücre büyümesi ve sekonder metabolit birikimindeki etkisini açıkça ortaya koymuştur.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | açıkgöz m, Batı Ay E, aygun a, Kara Ş (2022). Light-Mediated Biosynthesis of Phenylpropanoid Metabolites in Cell Suspension Cultures of Turkish Yarrow (Achillea gypsicola Hub. Mor.). , 1234 - 1242. 10.18016/ksutarimdoga.vi.926160 |
| Chicago | açıkgöz muhammed akif,Batı Ay Ebru,aygun ahmet,Kara Şevket Metin Light-Mediated Biosynthesis of Phenylpropanoid Metabolites in Cell Suspension Cultures of Turkish Yarrow (Achillea gypsicola Hub. Mor.). (2022): 1234 - 1242. 10.18016/ksutarimdoga.vi.926160 |
| MLA | açıkgöz muhammed akif,Batı Ay Ebru,aygun ahmet,Kara Şevket Metin Light-Mediated Biosynthesis of Phenylpropanoid Metabolites in Cell Suspension Cultures of Turkish Yarrow (Achillea gypsicola Hub. Mor.). , 2022, ss.1234 - 1242. 10.18016/ksutarimdoga.vi.926160 |
| AMA | açıkgöz m,Batı Ay E,aygun a,Kara Ş Light-Mediated Biosynthesis of Phenylpropanoid Metabolites in Cell Suspension Cultures of Turkish Yarrow (Achillea gypsicola Hub. Mor.). . 2022; 1234 - 1242. 10.18016/ksutarimdoga.vi.926160 |
| Vancouver | açıkgöz m,Batı Ay E,aygun a,Kara Ş Light-Mediated Biosynthesis of Phenylpropanoid Metabolites in Cell Suspension Cultures of Turkish Yarrow (Achillea gypsicola Hub. Mor.). . 2022; 1234 - 1242. 10.18016/ksutarimdoga.vi.926160 |
| IEEE | açıkgöz m,Batı Ay E,aygun a,Kara Ş "Light-Mediated Biosynthesis of Phenylpropanoid Metabolites in Cell Suspension Cultures of Turkish Yarrow (Achillea gypsicola Hub. Mor.)." , ss.1234 - 1242, 2022. 10.18016/ksutarimdoga.vi.926160 |
| ISNAD | açıkgöz, muhammed akif vd. "Light-Mediated Biosynthesis of Phenylpropanoid Metabolites in Cell Suspension Cultures of Turkish Yarrow (Achillea gypsicola Hub. Mor.)". (2022), 1234-1242. https://doi.org/10.18016/ksutarimdoga.vi.926160 |
| APA | açıkgöz m, Batı Ay E, aygun a, Kara Ş (2022). Light-Mediated Biosynthesis of Phenylpropanoid Metabolites in Cell Suspension Cultures of Turkish Yarrow (Achillea gypsicola Hub. Mor.). Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 25(6), 1234 - 1242. 10.18016/ksutarimdoga.vi.926160 |
| Chicago | açıkgöz muhammed akif,Batı Ay Ebru,aygun ahmet,Kara Şevket Metin Light-Mediated Biosynthesis of Phenylpropanoid Metabolites in Cell Suspension Cultures of Turkish Yarrow (Achillea gypsicola Hub. Mor.). Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 25, no.6 (2022): 1234 - 1242. 10.18016/ksutarimdoga.vi.926160 |
| MLA | açıkgöz muhammed akif,Batı Ay Ebru,aygun ahmet,Kara Şevket Metin Light-Mediated Biosynthesis of Phenylpropanoid Metabolites in Cell Suspension Cultures of Turkish Yarrow (Achillea gypsicola Hub. Mor.). Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, vol.25, no.6, 2022, ss.1234 - 1242. 10.18016/ksutarimdoga.vi.926160 |
| AMA | açıkgöz m,Batı Ay E,aygun a,Kara Ş Light-Mediated Biosynthesis of Phenylpropanoid Metabolites in Cell Suspension Cultures of Turkish Yarrow (Achillea gypsicola Hub. Mor.). Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi. 2022; 25(6): 1234 - 1242. 10.18016/ksutarimdoga.vi.926160 |
| Vancouver | açıkgöz m,Batı Ay E,aygun a,Kara Ş Light-Mediated Biosynthesis of Phenylpropanoid Metabolites in Cell Suspension Cultures of Turkish Yarrow (Achillea gypsicola Hub. Mor.). Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi. 2022; 25(6): 1234 - 1242. 10.18016/ksutarimdoga.vi.926160 |
| IEEE | açıkgöz m,Batı Ay E,aygun a,Kara Ş "Light-Mediated Biosynthesis of Phenylpropanoid Metabolites in Cell Suspension Cultures of Turkish Yarrow (Achillea gypsicola Hub. Mor.)." Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 25, ss.1234 - 1242, 2022. 10.18016/ksutarimdoga.vi.926160 |
| ISNAD | açıkgöz, muhammed akif vd. "Light-Mediated Biosynthesis of Phenylpropanoid Metabolites in Cell Suspension Cultures of Turkish Yarrow (Achillea gypsicola Hub. Mor.)". Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 25/6 (2022), 1234-1242. https://doi.org/10.18016/ksutarimdoga.vi.926160 |
