TY  - JOUR
TI  - Cryogenic X-ray crystallographic studies of biomacromolecules at Turkish Light Source “Turkish DeLight”
AB  - X-ray crystallography is a robust and powerful structural biology technique that provides high-resolution atomic structures of biomacromolecules. Scientists use this technique to unravel mechanistic and structural details of biological macromolecules (e.g., proteins, nucleic acids, protein complexes, protein-nucleic acid complexes, or large biological compartments). Since its inception, single-crystal cryocrystallography has never been performed in Türkiye due to the lack of a single-crystal X-ray diffractometer. The X-ray diffraction facility recently established at the University of Health Sciences, İstanbul, Türkiye will enable Turkish and international researchers to easily perform high-resolution structural analysis of biomacromolecules from single crystals. Here, we describe the technical and practical outlook of a state-of-the-art home-source X-ray, using lysozyme as a model protein. The methods and practice described in this article can be applied to any biological sample for structural studies. Therefore, this article will be a valuable practical guide from sample preparation to data analysis.
AU  - Goc, Gunce
AU  - Durdagi, Serdar
AU  - Karaca, Ezgi
AU  - Dinler Doganay, Gizem
AU  - Akcan, Enver Kamil
AU  - AYAN, ESRA
AU  - Destan, Ebru
AU  - TOKAY, NURETTİN
AU  - Çakılkaya, Barış
AU  - KEPCEOĞLU, Abdullah
AU  - Yapıcı, İlkin
AU  - Tosun, Bilge
AU  - Baldır, Nilüfer
AU  - YILDIRIM, GÜNSELİ
AU  - Johnson, J Austin
AU  - Arslan, Nazlı Eylül
AU  - YILMAZ, Merve
AU  - Kulakman, Cahine
AU  - Paydos, Seyide Seda
AU  - Çinal, Zeynep Sena
AU  - Şabanoğlu, Kardelen
AU  - Pazarçeviren, Ayşegül
AU  - Yılmaz, Ayşenur
AU  - Asci, Bengisu
AU  - Kartal, Esra
AU  - Tavlı, Serra
AU  - Fujita, Mikako
AU  - tekin, saban
AU  - Altuntas, Sevde
AU  - Ertem, Fatma Betul
AU  - Ciftci, Halilibrahim
AU  - Gul, Mehmet
AU  - Guven, Omur
AU  - Caliseki, Mehmet
AU  - Canbay, Zeynep Başak
AU  - Karakadıoğlu Usta, Gözde
AU  - NERGIZ, ZELIS
AU  - Yesilay, Gamze
AU  - Kaplan Türköz, Burcu
AU  - Kabasakal, Burak Veli
AU  - Demirci, Hasan
AU  - KATI, AHMET
AU  - Atalay, Necati
AU  - SHAFIEI, ALALEH
AU  - TATEISHI, HIROSHI
AU  - Otsuka, Masami
AU  - Mermer, Arif
DO  - 10.55730/1300-0152.2637
PY  - 2023
JO  - Turkish Journal of Biology
VL  - 47
IS  - 1
SN  - 1300-0152
SP  - 1
EP  - 13
DB  - TRDizin
UR  - http://search/yayin/detay/1159438
ER  -