Genotyping of the κ – casein gene by PCR – RFLP (AcuI digestion) and its relationship to milk yield traits in Anatolian buffaloes, Murrah buffaloes, and their crosses

Çetin, İlkay; Küçükkebabçı, Mustafa; SOYSAL, MEHMET IHSAN; Kader Esen, Vasfiye; KARADAĞ, ORHAN; Yüksel, Mehmet Akif; SEVIM, SEMIH; KAPTAN, Binnur

doi:10.55730/1300-0128.4268

Genotyping of the κ – casein gene by PCR – RFLP (AcuI digestion) and its relationship to milk yield traits in Anatolian buffaloes, Murrah buffaloes, and their crosses

Orhan KARADAĞ, (Muş Alparslan Üniversitesi, Uygulamalı Bilimler Fakültesi, Hayvansal Üretim ve Teknolojileri Bölümü, Muş, Türkiye)

Vasfiye KADER ESEN, (Koyun Islahı Araştırma Enstitüsü, Balıkesir, Türkiye)

Mehmet Akif YÜKSEL, (Koyun Islahı Araştırma Enstitüsü, Balıkesir, Türkiye)

Mustafa Küçükkebabçı, (TC Tarım ve Orman Bakanlığı, Karabük İl Tarım ve Orman Müdürlüğü, Karabük, Türkiye)

Semih SEVİM, (TC Tarım ve Orman Bakanlığı, Aydın İl Tarım ve Orman Müdürlüğü, Aydın, Türkiye)

İlkay ÇETİN, (Koyun Islahı Araştırma Enstitüsü, Balıkesir, Türkiye)

Binnur KAPTAN, (Namık Kemal Üniversitesi, Ziraat Fakültesi, Gıda Mühendisliği Bölümü, Tekirdağ, Türkiye)

Mehmet İHSANSOYSAL (Namık Kemal Üniversitesi, Ziraat Fakültesi, Zootekni Bölümü, Tekirdağ, Türkiye)

Turkish Journal of Veterinary and Animal Sciences

5 0

Yıl: 2023 Cilt: 47 Sayı: 1 Sayfa Aralığı: 55 - 61 Metin Dili: İngilizce DOI: 10.55730/1300-0128.4268 İndeks Tarihi: 23-03-2023

Genotyping of the κ – casein gene by PCR – RFLP (AcuI digestion) and its relationship to milk yield traits in Anatolian buffaloes, Murrah buffaloes, and their crosses

Öz:

The aim of this study was to investigate the polymorphism of the κ – casein gene (kappa casein, CSN3) in buffaloes and determine its effects on milk yield traits. Data were obtained from 2016 to 2019 from all animals in the ongoing Anatolian Buffalo Breeding Project. Genomic DNA was collected from a total of 209 buffaloes of different ages and sexes, namely 31 Murrah, 46 Anatolian buffaloes, and 132 of their crosses (Murrah × Anatolian). Genotyping of CSN3 gene by polymerase chain reaction–restriction fragment length polymorphism (PCR – RFLP) technique with restriction enzyme (AcuI) revealed three genotypes: AA (0.689), AB (0.282), and BB (0.028), and the frequencies of A and B alleles were 0.83 and 0.17, respectively. A total of 113 milk yield data and 74 milk component analyses of dairy buffaloes were used to compare CSN3 genotypes for milk yield traits. Statistical analysis showed that there was a significant difference in lactation milk yield between CSN3 genotypes: BB, 1560.3 ± 326.7 kg, AB, 1278.1 ± 111.3 kg, and AA, 1060.9 ± 85.6 kg (p < 0.05). However, the mean values of lactation length and milk components were not significant between genotypes (p > 0.05). These results suggest that these variations $(135^{ThrACC} / IleATC /136^{ThrACC} / ThrACT)$ in the buffalo CSN3 gene may be used as genetic markers in dairy buffalo breeding programs.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	KARADAĞ O, Kader Esen V, Yüksel M, Küçükkebabçı M, SEVIM S, Çetin İ, KAPTAN B, SOYSAL M (2023). Genotyping of the κ – casein gene by PCR – RFLP (AcuI digestion) and its relationship to milk yield traits in Anatolian buffaloes, Murrah buffaloes, and their crosses. , 55 - 61. 10.55730/1300-0128.4268
Chicago	KARADAĞ ORHAN,Kader Esen Vasfiye,Yüksel Mehmet Akif,Küçükkebabçı Mustafa,SEVIM SEMIH,Çetin İlkay,KAPTAN Binnur,SOYSAL MEHMET IHSAN Genotyping of the κ – casein gene by PCR – RFLP (AcuI digestion) and its relationship to milk yield traits in Anatolian buffaloes, Murrah buffaloes, and their crosses. (2023): 55 - 61. 10.55730/1300-0128.4268
MLA	KARADAĞ ORHAN,Kader Esen Vasfiye,Yüksel Mehmet Akif,Küçükkebabçı Mustafa,SEVIM SEMIH,Çetin İlkay,KAPTAN Binnur,SOYSAL MEHMET IHSAN Genotyping of the κ – casein gene by PCR – RFLP (AcuI digestion) and its relationship to milk yield traits in Anatolian buffaloes, Murrah buffaloes, and their crosses. , 2023, ss.55 - 61. 10.55730/1300-0128.4268
AMA	KARADAĞ O,Kader Esen V,Yüksel M,Küçükkebabçı M,SEVIM S,Çetin İ,KAPTAN B,SOYSAL M Genotyping of the κ – casein gene by PCR – RFLP (AcuI digestion) and its relationship to milk yield traits in Anatolian buffaloes, Murrah buffaloes, and their crosses. . 2023; 55 - 61. 10.55730/1300-0128.4268
Vancouver	KARADAĞ O,Kader Esen V,Yüksel M,Küçükkebabçı M,SEVIM S,Çetin İ,KAPTAN B,SOYSAL M Genotyping of the κ – casein gene by PCR – RFLP (AcuI digestion) and its relationship to milk yield traits in Anatolian buffaloes, Murrah buffaloes, and their crosses. . 2023; 55 - 61. 10.55730/1300-0128.4268
IEEE	KARADAĞ O,Kader Esen V,Yüksel M,Küçükkebabçı M,SEVIM S,Çetin İ,KAPTAN B,SOYSAL M "Genotyping of the κ – casein gene by PCR – RFLP (AcuI digestion) and its relationship to milk yield traits in Anatolian buffaloes, Murrah buffaloes, and their crosses." , ss.55 - 61, 2023. 10.55730/1300-0128.4268
ISNAD	KARADAĞ, ORHAN vd. "Genotyping of the κ – casein gene by PCR – RFLP (AcuI digestion) and its relationship to milk yield traits in Anatolian buffaloes, Murrah buffaloes, and their crosses". (2023), 55-61. https://doi.org/10.55730/1300-0128.4268

APA	KARADAĞ O, Kader Esen V, Yüksel M, Küçükkebabçı M, SEVIM S, Çetin İ, KAPTAN B, SOYSAL M (2023). Genotyping of the κ – casein gene by PCR – RFLP (AcuI digestion) and its relationship to milk yield traits in Anatolian buffaloes, Murrah buffaloes, and their crosses. Turkish Journal of Veterinary and Animal Sciences, 47(1), 55 - 61. 10.55730/1300-0128.4268
Chicago	KARADAĞ ORHAN,Kader Esen Vasfiye,Yüksel Mehmet Akif,Küçükkebabçı Mustafa,SEVIM SEMIH,Çetin İlkay,KAPTAN Binnur,SOYSAL MEHMET IHSAN Genotyping of the κ – casein gene by PCR – RFLP (AcuI digestion) and its relationship to milk yield traits in Anatolian buffaloes, Murrah buffaloes, and their crosses. Turkish Journal of Veterinary and Animal Sciences 47, no.1 (2023): 55 - 61. 10.55730/1300-0128.4268
MLA	KARADAĞ ORHAN,Kader Esen Vasfiye,Yüksel Mehmet Akif,Küçükkebabçı Mustafa,SEVIM SEMIH,Çetin İlkay,KAPTAN Binnur,SOYSAL MEHMET IHSAN Genotyping of the κ – casein gene by PCR – RFLP (AcuI digestion) and its relationship to milk yield traits in Anatolian buffaloes, Murrah buffaloes, and their crosses. Turkish Journal of Veterinary and Animal Sciences, vol.47, no.1, 2023, ss.55 - 61. 10.55730/1300-0128.4268
AMA	KARADAĞ O,Kader Esen V,Yüksel M,Küçükkebabçı M,SEVIM S,Çetin İ,KAPTAN B,SOYSAL M Genotyping of the κ – casein gene by PCR – RFLP (AcuI digestion) and its relationship to milk yield traits in Anatolian buffaloes, Murrah buffaloes, and their crosses. Turkish Journal of Veterinary and Animal Sciences. 2023; 47(1): 55 - 61. 10.55730/1300-0128.4268
Vancouver	KARADAĞ O,Kader Esen V,Yüksel M,Küçükkebabçı M,SEVIM S,Çetin İ,KAPTAN B,SOYSAL M Genotyping of the κ – casein gene by PCR – RFLP (AcuI digestion) and its relationship to milk yield traits in Anatolian buffaloes, Murrah buffaloes, and their crosses. Turkish Journal of Veterinary and Animal Sciences. 2023; 47(1): 55 - 61. 10.55730/1300-0128.4268
IEEE	KARADAĞ O,Kader Esen V,Yüksel M,Küçükkebabçı M,SEVIM S,Çetin İ,KAPTAN B,SOYSAL M "Genotyping of the κ – casein gene by PCR – RFLP (AcuI digestion) and its relationship to milk yield traits in Anatolian buffaloes, Murrah buffaloes, and their crosses." Turkish Journal of Veterinary and Animal Sciences, 47, ss.55 - 61, 2023. 10.55730/1300-0128.4268
ISNAD	KARADAĞ, ORHAN vd. "Genotyping of the κ – casein gene by PCR – RFLP (AcuI digestion) and its relationship to milk yield traits in Anatolian buffaloes, Murrah buffaloes, and their crosses". Turkish Journal of Veterinary and Animal Sciences 47/1 (2023), 55-61. https://doi.org/10.55730/1300-0128.4268