Investigation of taurine and aqueous garlic extract diet supplementation effect on the healing of rat osteoporotic fractures

Background/aim: We have evaluated the effects of taurine and aqueous garlic extract (AGE) as a dietary supplement on osteoporotic fracture (OPF) healing in the ovariectomized rat femur fracture model. Materials and methods: In this experimental animal study, twenty-four osteoporosis-remodeled female Wistar albino rats were randomly divided into 3 groups (n: 8) according to their supplemented diet; control, taurine, and AGE groups. Unilateral femur middiaphysis mini-open osteotomy was stabilized with Kirschner wires. Six weeks after osteotomy, the rats were sacrificed before the femurs were harvested and OPF healing was evaluated with biochemical, histologic, microcomputed-tomography, and scintigraphic methods. Results: As an indicator of the antiosteoporotic effect, the calcium levels of the taurine group were significantly lower than the AGE and control groups in biochemical analyzes (p < 0.01). In histological studies, the new bone diameter and new bone volume values of the taurine group were significantly higher than the control group (p = 0.002 and p = 0.032, respectively), while higher trabecular-compact callus was observed in the taurine and AGE groups, respectively, compared to the control group. In morphological analyses, taurine and AGE groups had significantly higher bone volume/tissue volume, trabecular number, bone surface density, and lower trabecular separation than the control group (p < 0.05). The scintigraphic imaging showed a significant increase in osteoblastic activity of the taurine group compared to the control group (p = 0.005). Conclusion: Taurine and AGE have positive anabolic effects, respectively, on the healing of OPFs, demonstrated by biochemical, histological, morphological, and scintigraphic methods.

Anahtar Kelime: Taurine aqueous garlic extract osteoporosis ovariectomized rat femur fracture

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1. Cheung WH, Miclau T, Chow SK, Yang FF, Alt V. Fracture healing in osteoporotic bone. Injury 2016; 47 (2)): 21-26. https://doi. org/ 10.1016/S0020-1383(16)47004-X
2. Mukherjee M, Das AS, Das D, Mukherjee S, Mitra S, Mitra C. Role of peritoneal macrophages and lymphocytes in the development of hypogonadal osteoporosis in an ovariectomized rat model: possible phytoestrogenic efficacy of oil extract of garlic to preserve skeletal health. Phytotherapy Research 2007; 21(11): 1045-1054. https://doi org/ 10.1002/ptr.2209
3. Ibrahim N, Mohamad S, Mohamed N, Shuid AN. Experimental fracture protocols in assessments of potential agents for osteoporotic fracture healing using rodent models. Current Drug Targets. 2013; 14(14): 1642-1650. https://doi org/ 10.21 74/1389450114666131216224003
4. Choi MJ. Taurine May Modulate Bone in Cholesterol Fed Estrogen Deficiency-Induced Rats. Advances in Experimental Medicine and Biology 2017; 975 Pt 2:1093-1102. https://doi org/ 10.1007/978-94-024-1079-2_87
5. Chen Y, Sun J, Dou C, Li N, Kang F et al. Alliin Attenuated RANKL- Induced Osteoclastogenesis by Scavenging Reactive Oxygen Species through Inhibiting Nox1. International Journal of Molecular Sciences 2016; 17(9): 1-13. https://doi org/ 10.3390/ ijms17091516
6. Cheong SH, Chang KJ. The preventive effect of fermented milk supplement containing tomato (Lycopersicon esculentum) and taurine on bone loss in ovariectomized rats. Advances in Experimental Medicine and Biology 2009; 643: 333-340. https://doi org/ 10.1007/978-0-387-75681-3_34
7. Putnam SE, Scutt AM, Bicknell K, Priestley CM, Williamson EM. Natural products as alternative treatments for metabolic bone disorders and for maintenance of bone health. Phytotherapy Research 2007; 21(2): 99-112. https://doi org/10.1002/ptr.2030
8. Gupta RC, Win T, Bittner S. Taurine analogs; a new class of therapeutics: retrospect and prospects. Current Medicinal Chemistry 2005; 12(17): 2021-2039. https://doi org/ 10.2174/0929867054546582
9. Roman-Garcia P, Quiros-Gonzalez I, Mottram L, Lieben L, Sharan K et al. Vitamin B₁₂-dependent taurine synthesis regulates growth and bone mass. The Journal of Clinical Investigation. 2014; 124(7): 2988-3002. https://doi org/ 10.1172/JCI72606
10. Wu G. Important roles of dietary taurine, creatine, carnosine, anserine and 4-hydroxyproline in human nutrition and health. Amino Acids 2020; 52(3): 329-360. https://doi org/ 10.1007/ s00726-020-02823-6
11. D’Eufemia P, Finocchiaro R, Celli M, Raccio I, Zambrano A et al. Taurine deficiency in thalassemia major-induced osteoporosis treated with neridronate. Biomedicine & Pharmacotherapy 2010; 64(4): 271-274. https://doi org/ 10.1016/j.biopha.2009.06.014
12. Choi MJ, Seo JN. Effect of taurine feeding on bone mineral density and bone markers in rats. Advances in Experimental Medicine and Biology 2013; 776: 51-58. https://doi org/ 10.1007/978-1- 4614-6093-0_6
13. Choi MJ, Chang KJ, Lee JW, Jung YJ. Beneficial Function of Taurine on Bone Metabolism in Alcohol-Fed OVX Rat Model. Advances in Experimental Medicine and Biology 2017; 975 Pt 2: 1059-1069. https://doi org/ 10.1007/978-94-024-1079-2_84
14. Van Stijn MF, Bruins AA, Vermeulen MA, Witlox J, Teerlink T et al. Effect of oral taurine on morbidity and mortality in elderly hip fracture patients: a randomized trial. International Journal of Molecular Sciences 2015; 16(6): 12288-12306. https://doi org/ 10.3390/ijms160612288
15. Ansary J, Forbes-Hernández TY, Gil E, Cianciosi D, Zhang J et al. Potential Health Benefit of Garlic Based on Human Intervention Studies: A Brief Overview. Antioxidants (Basel) 2020; 9(7): 619. https://doi org/ 10.3390/antiox9070619
16. Ohtani M, Nishimura T. The preventive and therapeutic application of garlic and other plant ingredients in the treatment of periodontal diseases. Experimental and Therapeutic Medicine 2020; 19(2): 1507-1510. https://doi org/ 10.3892/etm.2019.8382
17. Arreola R, Quintero-Fabián S, López-Roa RI, Flores-Gutiérrez EO, Reyes-Grajeda JP et al. Immunomodulation and anti- inflammatory effects of garlic compounds. Journal of Immunology Research 2015; 2015: 401630. https://doi org/ 10.1155/2015/401630
18. Yang J, Tang R, Yi J, Chen Y, Li X et al. Diallyl disulfide alleviates inflammatory osteolysis by suppressing osteoclastogenesis via NF-κB-NFATc1 signal pathway. FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology 2019; 33(6): 7261-7273. https://doi org/ 10.1096/fj.201802172R
19. Fu SW, Zeng GF, Zong SH, Zhang ZY, Zou B et al. Systematic review and meta-analysis of the bone protective effect of phytoestrogens on osteoporosis in ovariectomized rats. Nutrition Research (New York) 2014; 34(6): 467-477. https:// doi org/ 10.1016/j.nutres.2014.05.003
20. Ahmadian F, Mozaffari-Khosravi H, Azaraein MH, Faraji R, Zavar-Reza J. The effect of consumption of garlic tablet on proteins oxidation biomarkers in postmenopausal osteoporotic women: A randomized clinical trial. Electronic Physician 2017; 9(11): 5670-5675. https://doi org/ 10.19082/5670
21. Odiase DE, Osazee LO. Histological Effects Aqueous Extract of Allium sativum (Alliaceae) Bulb on Bone and Spleen of Adult Wistar Rats. Journal of Applied Sciences and Environmental Management 2017; 21(3): 538-544. https://doi org/ 10.4314/ jasem.v21i3.15
22. Kuo YJ, Sun JS, Rau G, Chen CH, Tsai TH, Tsuang YH. Better Osteoporotic Fracture Healing with Sintered Dicalcium Pyrophosphate (SDCP) Treatment: A Rat Femoral Fracture Model. The Journal of Histochemistry and Cytochemistry 2014; 62(8): 565-576. https://doi org/ 10.1369/0022155414538264
23. Özmeriç A, Tanoğlu O, Ocak M, Çelik HH, Fırat A et al. Intramedullary implants coated with cubic boron nitride enhance bone fracture healing in a rat model. Journal of Trace Elements in Medicine and Biology 2020; 62: 126599. https:// doi org/10.1016/j.jtemb.2020. 126599
24- Santić V, Cvek SZ, Sestan B, Bobinac D, Tudor A et al. Treatment of tibial bone defect with rotational vascular periosteal graft in rabbits. Collegium Antropologicum 2009; 33(1): 43-50. PMID: 19408602
25. Liu Y, You M, Shen J, Xu Y, Li L et al. Allicin Reversed the Process of Frailty in Aging Male Fischer 344 Rats With Osteoporosis. The Journal of Gerontology Series A, Biological Sciences and Medical Sciences 2020; 75(5): 821-825. https://doi org/ 10.1093/gerona/glz205
26. Moon PD, Kim MH, Lim HS, Oh HA, Nam SY et al. Taurine, a major amino acid of oyster, enhances linear bone growth in a mouse model of protein malnutrition. BioFactors 2015; 41(3): 190-197. https://doi org/ 10.1002/biof.1213
27. Cheng C, Alt V, Pan L, Thormann U, Schnettler R et al. Preliminary evaluation of different biomaterials for defect healing in an experimental osteoporotic rat model with dynamic PET-CT (dPET-CT) using F-18-sodium fluoride (NaF). Injury 2014; 45(3): 501-505. https://doi org/ 10.1016/j.injury.2013.11.023

APA	MISIRLIOĞLU M, bingol i, GENC C, AKBULUT A, ocak m, Diker N, OZMERİC A, Koca G, Kaya Bahçecitapar M, Şeneş M, Firat A, KAYMAZ F, Çelik H, TATLI ÇANKAYA İ, KORKMAZ M (2023). Investigation of taurine and aqueous garlic extract diet supplementation effect on the healing of rat osteoporotic fractures. , 29 - 39. 10.55730/1300-0144.5555
Chicago	MISIRLIOĞLU MESUT,bingol izzet,GENC COSKUN,AKBULUT Aylin,ocak mert,Diker Neziha Yağmur,OZMERİC AHMET,Koca Gökhan,Kaya Bahçecitapar Melike,Şeneş Mehmet,Firat Aysegul,KAYMAZ FEVZIYE FIGEN,Çelik Hakan Hamdi,TATLI ÇANKAYA İFFET İREM,KORKMAZ MELIHA Investigation of taurine and aqueous garlic extract diet supplementation effect on the healing of rat osteoporotic fractures. (2023): 29 - 39. 10.55730/1300-0144.5555
MLA	MISIRLIOĞLU MESUT,bingol izzet,GENC COSKUN,AKBULUT Aylin,ocak mert,Diker Neziha Yağmur,OZMERİC AHMET,Koca Gökhan,Kaya Bahçecitapar Melike,Şeneş Mehmet,Firat Aysegul,KAYMAZ FEVZIYE FIGEN,Çelik Hakan Hamdi,TATLI ÇANKAYA İFFET İREM,KORKMAZ MELIHA Investigation of taurine and aqueous garlic extract diet supplementation effect on the healing of rat osteoporotic fractures. , 2023, ss.29 - 39. 10.55730/1300-0144.5555
AMA	MISIRLIOĞLU M,bingol i,GENC C,AKBULUT A,ocak m,Diker N,OZMERİC A,Koca G,Kaya Bahçecitapar M,Şeneş M,Firat A,KAYMAZ F,Çelik H,TATLI ÇANKAYA İ,KORKMAZ M Investigation of taurine and aqueous garlic extract diet supplementation effect on the healing of rat osteoporotic fractures. . 2023; 29 - 39. 10.55730/1300-0144.5555
Vancouver	MISIRLIOĞLU M,bingol i,GENC C,AKBULUT A,ocak m,Diker N,OZMERİC A,Koca G,Kaya Bahçecitapar M,Şeneş M,Firat A,KAYMAZ F,Çelik H,TATLI ÇANKAYA İ,KORKMAZ M Investigation of taurine and aqueous garlic extract diet supplementation effect on the healing of rat osteoporotic fractures. . 2023; 29 - 39. 10.55730/1300-0144.5555
IEEE	MISIRLIOĞLU M,bingol i,GENC C,AKBULUT A,ocak m,Diker N,OZMERİC A,Koca G,Kaya Bahçecitapar M,Şeneş M,Firat A,KAYMAZ F,Çelik H,TATLI ÇANKAYA İ,KORKMAZ M "Investigation of taurine and aqueous garlic extract diet supplementation effect on the healing of rat osteoporotic fractures." , ss.29 - 39, 2023. 10.55730/1300-0144.5555
ISNAD	MISIRLIOĞLU, MESUT vd. "Investigation of taurine and aqueous garlic extract diet supplementation effect on the healing of rat osteoporotic fractures". (2023), 29-39. https://doi.org/10.55730/1300-0144.5555

APA	MISIRLIOĞLU M, bingol i, GENC C, AKBULUT A, ocak m, Diker N, OZMERİC A, Koca G, Kaya Bahçecitapar M, Şeneş M, Firat A, KAYMAZ F, Çelik H, TATLI ÇANKAYA İ, KORKMAZ M (2023). Investigation of taurine and aqueous garlic extract diet supplementation effect on the healing of rat osteoporotic fractures. Turkish Journal of Medical Sciences, 53(1), 29 - 39. 10.55730/1300-0144.5555
Chicago	MISIRLIOĞLU MESUT,bingol izzet,GENC COSKUN,AKBULUT Aylin,ocak mert,Diker Neziha Yağmur,OZMERİC AHMET,Koca Gökhan,Kaya Bahçecitapar Melike,Şeneş Mehmet,Firat Aysegul,KAYMAZ FEVZIYE FIGEN,Çelik Hakan Hamdi,TATLI ÇANKAYA İFFET İREM,KORKMAZ MELIHA Investigation of taurine and aqueous garlic extract diet supplementation effect on the healing of rat osteoporotic fractures. Turkish Journal of Medical Sciences 53, no.1 (2023): 29 - 39. 10.55730/1300-0144.5555
MLA	MISIRLIOĞLU MESUT,bingol izzet,GENC COSKUN,AKBULUT Aylin,ocak mert,Diker Neziha Yağmur,OZMERİC AHMET,Koca Gökhan,Kaya Bahçecitapar Melike,Şeneş Mehmet,Firat Aysegul,KAYMAZ FEVZIYE FIGEN,Çelik Hakan Hamdi,TATLI ÇANKAYA İFFET İREM,KORKMAZ MELIHA Investigation of taurine and aqueous garlic extract diet supplementation effect on the healing of rat osteoporotic fractures. Turkish Journal of Medical Sciences, vol.53, no.1, 2023, ss.29 - 39. 10.55730/1300-0144.5555
AMA	MISIRLIOĞLU M,bingol i,GENC C,AKBULUT A,ocak m,Diker N,OZMERİC A,Koca G,Kaya Bahçecitapar M,Şeneş M,Firat A,KAYMAZ F,Çelik H,TATLI ÇANKAYA İ,KORKMAZ M Investigation of taurine and aqueous garlic extract diet supplementation effect on the healing of rat osteoporotic fractures. Turkish Journal of Medical Sciences. 2023; 53(1): 29 - 39. 10.55730/1300-0144.5555
Vancouver	MISIRLIOĞLU M,bingol i,GENC C,AKBULUT A,ocak m,Diker N,OZMERİC A,Koca G,Kaya Bahçecitapar M,Şeneş M,Firat A,KAYMAZ F,Çelik H,TATLI ÇANKAYA İ,KORKMAZ M Investigation of taurine and aqueous garlic extract diet supplementation effect on the healing of rat osteoporotic fractures. Turkish Journal of Medical Sciences. 2023; 53(1): 29 - 39. 10.55730/1300-0144.5555
IEEE	MISIRLIOĞLU M,bingol i,GENC C,AKBULUT A,ocak m,Diker N,OZMERİC A,Koca G,Kaya Bahçecitapar M,Şeneş M,Firat A,KAYMAZ F,Çelik H,TATLI ÇANKAYA İ,KORKMAZ M "Investigation of taurine and aqueous garlic extract diet supplementation effect on the healing of rat osteoporotic fractures." Turkish Journal of Medical Sciences, 53, ss.29 - 39, 2023. 10.55730/1300-0144.5555
ISNAD	MISIRLIOĞLU, MESUT vd. "Investigation of taurine and aqueous garlic extract diet supplementation effect on the healing of rat osteoporotic fractures". Turkish Journal of Medical Sciences 53/1 (2023), 29-39. https://doi.org/10.55730/1300-0144.5555