This study aimed to determine beta hydroxybutyric acid (BHBA), vitamin B12, folate and glucose levels in Aleppo goats with pregnancy toxemia and to reveal their relationship with the disease. The study included 54 Aleppo goats ranging in age from one to four years old and in the last six weeks of pregnancy. Clinical examinations and BHBA measurements of all goats were performed. Goats are divided into 3 different groups based on their blood β-hydroxybutyrate (BHBA) as follows: non-ketonemic (<0.8 mmol/L, n= 15), moderate hyperketonemic (0.8-1.6 mmol/L, n= 13) and severe hyperketonemic (>1.6 mmol/L, n= 26). While no significant difference was found between the non-ketonemic group and moderate hyperketonemic group in BHBA concentration (P>0.05), a significant difference was found between the non-ketonemic group and the severe hyperketonemic group (P<0.001). The difference in vitamin B12 concentration between the 3 groups was significant (P<0.005). There was no significant difference between the 3 groups in terms of folate and glucose values (P>0.05). In the correlation graph between the variables in goats with pregnancy toxemia, a positive correlation was observed between BHBA concentration and vitamin B12 (r=0.392, P<0.05) and glucose (r=0.394, P<0.05). In addition, a positive correlation was observed between vitamin B12 value and folate (r=0.206, P<0.05) and glucose (r=0.323, P<0.05). In conclusion, our results suggest that hyperketonemia associated with serum B12 and glucose concentrations. Future studies are needed to understand the underlying mechanism of B12 increase in goats with pregnancy toxemia.