Objective: Antibiotic resistance development in the treatment of Acinetobacter infection is a serious health care problem and responsible for high mortality in intensive care units (ICU). In our study, it was aimed to determine rates of antibiotics resistance of Acinetobacter strains isolated from various samples in our ICU. Methods: We examined the records of Acinetobacter isolates and antibiotics resistance for one year followed up in our ICU. The samples from different patients and different type of samples of the same patients were evaluated. The data was analyzed with SPSS for Windows version 23.0. Categorical variables were expressed in terms of numbers and percentage. Results: 50% of the samples were isolated from tracheostomy. 96.4% of the 138 isolates were Acinetobacter baumannii and 3.6% were the other strains. We found high resistance to all of antibiotics except colistin (3.6%) and tigecycline (13.1%). Conclusion: Acinetobacter is the most important opportunistic human pathogen causing fatal nosocomial infections because its ability of developing resistance to new antibiotics is overly fast. Compared to the results reported from Dicle University Hospital in south east of our country it was determined that antibiotics resistance, especially colistin resistance ratio in our ICU was different. It is important to remember that antibiotic susceptibility may vary in regions, hospitals and even clinics, and resistance development should be constantly detected to make the appropriate initial therapy until deescalation