DETERMINATION OF THE EFFECT OF RUTIN ON EPITHELIAL TO MESENCHYMAL TRANSITION IN PROSTATE CANCER CELLS

Bal, Esra; Deveci Özkan, Asuman; Betts, Zeynep

doi:10.53446/actamednicomedia.1171654

DETERMINATION OF THE EFFECT OF RUTIN ON EPITHELIAL TO MESENCHYMAL TRANSITION IN PROSTATE CANCER CELLS

Esra BAL, (Kocaeli Üniversitesi, Fen Bilimleri Enstitüsü, Biyoloji Anabilim Dalı, Kocaeli, Türkiye)

Asuman DEVECİ ÖZKAN, (Sakarya Üniversitesi, Tıp Fakültesi, Tıbbi Biyoloji Anabilim Dalı, Sakarya, Türkiye)

Zeynep BETTS (Kocaeli Üniversitesi, Fen-Edebiyat Fakültesi, Biyoloji Bölümü, Kocaeli, Türkiye)

Acta Medica Nicomedia

6 3

Yıl: 2023 Cilt: 6 Sayı: 1 Sayfa Aralığı: 131 - 136 Metin Dili: İngilizce DOI: 10.53446/actamednicomedia.1171654 İndeks Tarihi: 16-06-2023

DETERMINATION OF THE EFFECT OF RUTIN ON EPITHELIAL TO MESENCHYMAL TRANSITION IN PROSTATE CANCER CELLS

Öz:

Objective: The difficulties experienced in the treatment of prostate cancer and the excess of side effects due to chemotherapy have brought the search for alternative treatment strategies. In recent studies, it is known that Rutin (RUT) has an anti-cancer effect on cancer cells. Our study aimed to determine the effects of RUT on epithelial to mesenchymal transition (EMT) in prostate cancer cells, for the first time. Methods: The anticancer effect of RUT in prostate cancer cells (PC-3) was examined by WST-1, Annexin V ELISA, DAPI and Acridine Orange staining, and the anti-cancer and anti-metastatic properties of RUT were evaluated with the Scratch Assay test. The expression level of Bax, Bcl-2, Snail, Twist, Vimentin and Ecadherin genes was evaluated by RT-PCR. Results: The cells were treated with RUT (500, 750, 1000 and 1500 µM) for 24 and 48 hours. The viability rates decreased with increasing RUT concentration depending on dose and time (p<0.01). Free Annexin V protein decreased, and apoptotic cells increased depending on the increasing RUT concentration (p<0.01). There was a reduction in the migrating cells, especially at 750 and 1000 µM RUT concentrations. There was a significant increase (14.22-fold) in E-cadherin mRNA level after 1500 µM RUT treatment (p<0.01). Additionally, the mRNA level of Snail, Twist and Vimentin decreased at higher RUT concentrations (p<0.01). Conclusion: The existence of RUT's potential to inhibit epithelial to mesenchymal transition and promote apoptosis has been demonstrated. It is also recommended to explore the effects of RUT on EMT in vivo

Anahtar Kelime: Prostate cancer rutin epithelial to mesenchymal transition apoptosis

EPİTELYALDEN MEZENKİMALE GEÇİŞ ÜZERİNE RUTİNİN ETKİLERİNİN PROSTAT KANSERİ HÜCRELERİNDE BELİRLENMESİ

Öz:

Amaç: Prostat kanseri tedavisinde yaşanan zorluklar, kemoterapi nedeniyle görülen yan etkilerin fazlalığı alternatif tedavi stratejileri arayışını gündeme getirmiştir. Son yıllarda yapılan araştırmalarda Rutin (RUT)’in kanser hücreleri üzerinde anti- kanser etki gösterdiği bilinmektedir. Bu nedenle çalışmamızda RUT’un prostat kanseri hücrelerinde epitelyalden mezenkimale geçiş (EMT) üzerindeki etkilerinin ilk defa belirlenmesi amaçlanmıştır. Yöntem: Prostat kanseri hücrelerinde (PC-3) RUT’un antikanser aktivitesi WST-1, Annexin V ELISA, DAPI boyama ve Akridin Oranj (AO) boyama ile belirlenerek, RUT’un anti-kanser ve anti- metastatik özellikleri Scratch (Çizik) Assay testi ile değerlendirildi. Bax, Bcl-2, Snail, Twist, Vimentin ve E-kaderin genlerinin mRNA ifade düzeyi RT-PCR analizi ile belirlendi. Bulgular: PC-3 hücrelerine, farklı konstrasyonlarda (500, 750, 1000 ve 1500 μM) ve farklı zamanlarda (24 ve 48 saat) RUT uygulandı. Canlılık oranlarının doza ve zamana bağlı olarak artan RUT konsantrasyonuna bağlı olarak azaldığı gözlendi (p<0.01). Ayrıca artan RUT konsantrasyonuna bağlı olarak serbest Annexin V proteinin azaldığı ve apoptotik hücrelerin arttığı belirlendi (p<0.01). Özellikle 750 ve 1000 μM RUT konsantrasyonlarında göç eden hücre sayısında azalma olduğu ortaya kondu. RT-PCR analizi sonucu elde edilen verilerde 1500 μM RUT muamelesi sonrası E- Kaderin mRNA seviyesinde 14.22-kat olarak belirgin bir anlamlı artış olduğu belirlendi (p<0.01). Diğer yandan EMT sürecinde yer aldığı bilinen Snail, Twist ve Vimentin mRNA ifade düzeyinin artan RUT konsantrasyonuna bağlı olarak azaldığı ortaya konuldu (p<0.01). Sonuç: RUT’un epitelden mezenkimal geçişi engelleme ve apoptozu teşvik edici potansiyelinin varlığı ortaya konulmuştur. Ayrıca RUT’un EMT üzerine olan etkilerinin daha ileri moleküler analizler ve in vivo olarak araştırılması önerilmektedir.

Anahtar Kelime: Prostat kanseri rutin epitelyalden

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Bal E, Deveci Özkan A, Betts Z (2023). DETERMINATION OF THE EFFECT OF RUTIN ON EPITHELIAL TO MESENCHYMAL TRANSITION IN PROSTATE CANCER CELLS. , 131 - 136. 10.53446/actamednicomedia.1171654
Chicago	Bal Esra,Deveci Özkan Asuman,Betts Zeynep DETERMINATION OF THE EFFECT OF RUTIN ON EPITHELIAL TO MESENCHYMAL TRANSITION IN PROSTATE CANCER CELLS. (2023): 131 - 136. 10.53446/actamednicomedia.1171654
MLA	Bal Esra,Deveci Özkan Asuman,Betts Zeynep DETERMINATION OF THE EFFECT OF RUTIN ON EPITHELIAL TO MESENCHYMAL TRANSITION IN PROSTATE CANCER CELLS. , 2023, ss.131 - 136. 10.53446/actamednicomedia.1171654
AMA	Bal E,Deveci Özkan A,Betts Z DETERMINATION OF THE EFFECT OF RUTIN ON EPITHELIAL TO MESENCHYMAL TRANSITION IN PROSTATE CANCER CELLS. . 2023; 131 - 136. 10.53446/actamednicomedia.1171654
Vancouver	Bal E,Deveci Özkan A,Betts Z DETERMINATION OF THE EFFECT OF RUTIN ON EPITHELIAL TO MESENCHYMAL TRANSITION IN PROSTATE CANCER CELLS. . 2023; 131 - 136. 10.53446/actamednicomedia.1171654
IEEE	Bal E,Deveci Özkan A,Betts Z "DETERMINATION OF THE EFFECT OF RUTIN ON EPITHELIAL TO MESENCHYMAL TRANSITION IN PROSTATE CANCER CELLS." , ss.131 - 136, 2023. 10.53446/actamednicomedia.1171654
ISNAD	Bal, Esra vd. "DETERMINATION OF THE EFFECT OF RUTIN ON EPITHELIAL TO MESENCHYMAL TRANSITION IN PROSTATE CANCER CELLS". (2023), 131-136. https://doi.org/10.53446/actamednicomedia.1171654

APA	Bal E, Deveci Özkan A, Betts Z (2023). DETERMINATION OF THE EFFECT OF RUTIN ON EPITHELIAL TO MESENCHYMAL TRANSITION IN PROSTATE CANCER CELLS. Acta Medica Nicomedia, 6(1), 131 - 136. 10.53446/actamednicomedia.1171654
Chicago	Bal Esra,Deveci Özkan Asuman,Betts Zeynep DETERMINATION OF THE EFFECT OF RUTIN ON EPITHELIAL TO MESENCHYMAL TRANSITION IN PROSTATE CANCER CELLS. Acta Medica Nicomedia 6, no.1 (2023): 131 - 136. 10.53446/actamednicomedia.1171654
MLA	Bal Esra,Deveci Özkan Asuman,Betts Zeynep DETERMINATION OF THE EFFECT OF RUTIN ON EPITHELIAL TO MESENCHYMAL TRANSITION IN PROSTATE CANCER CELLS. Acta Medica Nicomedia, vol.6, no.1, 2023, ss.131 - 136. 10.53446/actamednicomedia.1171654
AMA	Bal E,Deveci Özkan A,Betts Z DETERMINATION OF THE EFFECT OF RUTIN ON EPITHELIAL TO MESENCHYMAL TRANSITION IN PROSTATE CANCER CELLS. Acta Medica Nicomedia. 2023; 6(1): 131 - 136. 10.53446/actamednicomedia.1171654
Vancouver	Bal E,Deveci Özkan A,Betts Z DETERMINATION OF THE EFFECT OF RUTIN ON EPITHELIAL TO MESENCHYMAL TRANSITION IN PROSTATE CANCER CELLS. Acta Medica Nicomedia. 2023; 6(1): 131 - 136. 10.53446/actamednicomedia.1171654
IEEE	Bal E,Deveci Özkan A,Betts Z "DETERMINATION OF THE EFFECT OF RUTIN ON EPITHELIAL TO MESENCHYMAL TRANSITION IN PROSTATE CANCER CELLS." Acta Medica Nicomedia, 6, ss.131 - 136, 2023. 10.53446/actamednicomedia.1171654
ISNAD	Bal, Esra vd. "DETERMINATION OF THE EFFECT OF RUTIN ON EPITHELIAL TO MESENCHYMAL TRANSITION IN PROSTATE CANCER CELLS". Acta Medica Nicomedia 6/1 (2023), 131-136. https://doi.org/10.53446/actamednicomedia.1171654