Analysis of Atenolol in Rabbit Plasma by HPLC Method
Yıl: 2023 Cilt: 16 Sayı: 1 Sayfa Aralığı: 1 - 10 Metin Dili: İngilizce DOI: 10.18185/erzifbed.1083703 İndeks Tarihi: 31-05-2023
Analysis of Atenolol in Rabbit Plasma by HPLC Method
Öz: The goal of this research is to develop a high-performance liquid chromatography method for analyzing atenolol levels in rabbit plasma and apply this method to the pharmacokinetic study. The liquid-liquid extraction technique was used to prepare blood samples from rabbits. Separation of atenolol was achieved on an $Ace C_{18}$ column. The method’s calibration curve was plotted between 5 and 250 ng/mL. The accuracy results were better than 2.97% and the precision results were less than 6.30% in rabbit plasma for atenolol. The method had recovery values >95.8% for all samples in rabbit plasma. In addition, the validated method was used to study atenolol pharmacokinetics in rabbits. The maximum atenolol plasma concentration is 240.1 ± 33.41 ng/mL. The duration to attain the greatest atenolol concentration and the area under the curve from$(AUC_{0-12 h})$were 3.0 ± 0.64 h and 1184.1 ± 235.13 ng/mL h, respectively.
Anahtar Kelime: Tavşan Plazmasında Atenololün HPLC Yöntemi ile Analizi
Öz: Atenolol günümüzde en çok kullanılan ilaçlardan biridir. Bu nedenle, atenolol tayini için yeni ve basit yöntemlerin geliştirilmesi önemlidir. Bu araştırmanın amacı tavşan plazmasında atenololün analizi için bir yüksek performanslı sıvı kromatografi yöntemi geliştirmek ve farmakokinetik çalışmaya uygulamaktır. Tavşan kan örnekleri sıvı-sıvı ekstraksiyon yöntemi ile hazırlanmıştır. Atenololün ayırımı $Ace C_{18}$ kolon ile yapılmıştır. Yöntemin kalibrasyon eğrisi 5 ve 250 ng/ng/ arasında çizildi. Tavşan plazmasında atenolün kesinlik sonuçları %6.30’dan küçüktü ve doğruluk sonuçları %2.97’den daha iyiydi. Yöntemin tavşan plazmasındaki tüm örnekler için geri kazanım değerleri >%93.1’dir. Ayrıca, tavşanlarda atenololün farmakokinetiğini incelemek için valide edilen yöntem uygulanmıştır. Maksimum atenolol plazma konsantrasyonu 240.1 ± 33.41 ng/mL idi. Atenololün maksimum konsantrasyona ulaşma süresi ve eğrinin altındaki alan $(AUC_{0-12 h})$ sırasıyla 3.0 ± 0.64 h ve 1184.1 ± 235.13 ng/mL h idi.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | Yılmaz B (2023). Analysis of Atenolol in Rabbit Plasma by HPLC Method. , 1 - 10. 10.18185/erzifbed.1083703 |
| Chicago | Yılmaz Bilal Analysis of Atenolol in Rabbit Plasma by HPLC Method. (2023): 1 - 10. 10.18185/erzifbed.1083703 |
| MLA | Yılmaz Bilal Analysis of Atenolol in Rabbit Plasma by HPLC Method. , 2023, ss.1 - 10. 10.18185/erzifbed.1083703 |
| AMA | Yılmaz B Analysis of Atenolol in Rabbit Plasma by HPLC Method. . 2023; 1 - 10. 10.18185/erzifbed.1083703 |
| Vancouver | Yılmaz B Analysis of Atenolol in Rabbit Plasma by HPLC Method. . 2023; 1 - 10. 10.18185/erzifbed.1083703 |
| IEEE | Yılmaz B "Analysis of Atenolol in Rabbit Plasma by HPLC Method." , ss.1 - 10, 2023. 10.18185/erzifbed.1083703 |
| ISNAD | Yılmaz, Bilal. "Analysis of Atenolol in Rabbit Plasma by HPLC Method". (2023), 1-10. https://doi.org/10.18185/erzifbed.1083703 |
| APA | Yılmaz B (2023). Analysis of Atenolol in Rabbit Plasma by HPLC Method. Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 16(1), 1 - 10. 10.18185/erzifbed.1083703 |
| Chicago | Yılmaz Bilal Analysis of Atenolol in Rabbit Plasma by HPLC Method. Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi 16, no.1 (2023): 1 - 10. 10.18185/erzifbed.1083703 |
| MLA | Yılmaz Bilal Analysis of Atenolol in Rabbit Plasma by HPLC Method. Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi, vol.16, no.1, 2023, ss.1 - 10. 10.18185/erzifbed.1083703 |
| AMA | Yılmaz B Analysis of Atenolol in Rabbit Plasma by HPLC Method. Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi. 2023; 16(1): 1 - 10. 10.18185/erzifbed.1083703 |
| Vancouver | Yılmaz B Analysis of Atenolol in Rabbit Plasma by HPLC Method. Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi. 2023; 16(1): 1 - 10. 10.18185/erzifbed.1083703 |
| IEEE | Yılmaz B "Analysis of Atenolol in Rabbit Plasma by HPLC Method." Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 16, ss.1 - 10, 2023. 10.18185/erzifbed.1083703 |
| ISNAD | Yılmaz, Bilal. "Analysis of Atenolol in Rabbit Plasma by HPLC Method". Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi 16/1 (2023), 1-10. https://doi.org/10.18185/erzifbed.1083703 |
