Urban settlements, one of the most important developments in the history of humanity, expanded due to the migration movements from rural to urban areas and turned into dense and high-built settlements. This situation has caused urban areas to have different climatic conditions from the suburban and rural areas around them. Bioclimatic comfort is the state of people to feel comfortable, happy and fit in the atmospheric environment they are in. Uncomfortable conditions cause much negativity such as decrease in people's work efficiency, health conditions and increase in energy consumption. Amasya is a small Anatolian city in the Central Black Sea Region of the Black Sea Region, where industrialization has not developed. In this study, hourly data of the year 2021 of two meteorology stations, which are considered as urban and suburban, were used in order to examine the effects of urban areas on bioclimatic comfort conditions in Amasya. As a method, the PET (Physiological Equivalent Temperature) index obtained from the RayMan model, which calculates many factors together, was used. As a result of the study, suburban is 2.1 ˚C cooler than urban at the general PET average; 3.4˚C cooler at the maximum average and 2.8˚C cooler at the minimum average. The urban area is exposed to heat stress by 8.1% more throughout the year than the suburban area. In order to reduce the negative bioclimatic comfort conditions of cities and for sustainable urbanization, it is necessary to make urban design and planning that takes into account human, ecological and physical conditions.