The Effect of Two Different Botulinum Neurotoxin A On The Cortical Neuron Cells In Terms of Apoptosis and MMP 2, MMP 7, and MMP9 Localizations

Zeynep Deniz ŞAHİN İNAN, (Sivas Cumhuriyet Üniversitesi, Tıp Fakültesi, Histoloji-Embriyoloji Anabilim Dalı, Sivas, Türkiye)
Zübeyda Akın Polat, (Sivas Cumhuriyet Üniversitesi, Tıp Fakültesi, Parazitoloji Anabilim Dalı, Sivas, Türkiye)
Rasim HAMUTOĞLU (Sivas Cumhuriyet Üniversitesi, Tıp Fakültesi, Histoloji-Embriyoloji Anabilim Dalı, Sivas, Türkiye)
Cumhuriyet Science Journal
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Yıl: 2023 Cilt: 44 Sayı: 1 Sayfa Aralığı: 19 - 27 Metin Dili: İngilizce DOI: 10.17776/csj.1180547 İndeks Tarihi: 25-05-2023

The Effect of Two Different Botulinum Neurotoxin A On The Cortical Neuron Cells In Terms of Apoptosis and MMP 2, MMP 7, and MMP9 Localizations

Öz:
This study aimed to associate the possible cytotoxic and apoptotic effects of Botox (Allergan) and Dysport (Ipsen) and immunolocalization of matrix metalloproteinase (MMP) proteins with HCN2 cortical neuron cell line. Accordingly, cytotoxic potentials of Botox and Dysport were determined on different concentrations. Then, the apoptosis rates of these cells were evaluated by TUNEL method. MMP2, MMP7, and MMP9 proteins were also visualized using immunofluorescence method. There was a significant difference in cytotoxicity between those treated with 3.2, 6.4, and 12.8IU compared with the control. The Dysport 12.8 IU group was statistically more toxic than Botox group at the same concentration. Therefore, the number of apoptotic cells increased from 0.1 IU in Botox and 0.01 IU in Dysport groups compared to the control. The number of apoptotic cells was significantly higher in Dysport group at 1.6, 3.2, 6.4, and 12.8 IU concentrations than in Botox group. It was determined that MMPs increased gradually at the concentrations where the number of apoptotic cells was highest compared to the control group. As a result, we consider that it may be necessary to deal with the dose adjustment in Botox and Dysport applications, together with detailed studies to be carried out in the future.
Anahtar Kelime: Apoptosis Botulinum neurotoxin A HCN2 MMP TUNEL.

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA SAHIN INAN Z, Akın Polat Z, HAMUTOGLU R (2023). The Effect of Two Different Botulinum Neurotoxin A On The Cortical Neuron Cells In Terms of Apoptosis and MMP 2, MMP 7, and MMP9 Localizations. , 19 - 27. 10.17776/csj.1180547
Chicago SAHIN INAN ZEYNEP DENIZ,Akın Polat Zübeyda,HAMUTOGLU RASIM The Effect of Two Different Botulinum Neurotoxin A On The Cortical Neuron Cells In Terms of Apoptosis and MMP 2, MMP 7, and MMP9 Localizations. (2023): 19 - 27. 10.17776/csj.1180547
MLA SAHIN INAN ZEYNEP DENIZ,Akın Polat Zübeyda,HAMUTOGLU RASIM The Effect of Two Different Botulinum Neurotoxin A On The Cortical Neuron Cells In Terms of Apoptosis and MMP 2, MMP 7, and MMP9 Localizations. , 2023, ss.19 - 27. 10.17776/csj.1180547
AMA SAHIN INAN Z,Akın Polat Z,HAMUTOGLU R The Effect of Two Different Botulinum Neurotoxin A On The Cortical Neuron Cells In Terms of Apoptosis and MMP 2, MMP 7, and MMP9 Localizations. . 2023; 19 - 27. 10.17776/csj.1180547
Vancouver SAHIN INAN Z,Akın Polat Z,HAMUTOGLU R The Effect of Two Different Botulinum Neurotoxin A On The Cortical Neuron Cells In Terms of Apoptosis and MMP 2, MMP 7, and MMP9 Localizations. . 2023; 19 - 27. 10.17776/csj.1180547
IEEE SAHIN INAN Z,Akın Polat Z,HAMUTOGLU R "The Effect of Two Different Botulinum Neurotoxin A On The Cortical Neuron Cells In Terms of Apoptosis and MMP 2, MMP 7, and MMP9 Localizations." , ss.19 - 27, 2023. 10.17776/csj.1180547
ISNAD SAHIN INAN, ZEYNEP DENIZ vd. "The Effect of Two Different Botulinum Neurotoxin A On The Cortical Neuron Cells In Terms of Apoptosis and MMP 2, MMP 7, and MMP9 Localizations". (2023), 19-27. https://doi.org/10.17776/csj.1180547
APA SAHIN INAN Z, Akın Polat Z, HAMUTOGLU R (2023). The Effect of Two Different Botulinum Neurotoxin A On The Cortical Neuron Cells In Terms of Apoptosis and MMP 2, MMP 7, and MMP9 Localizations. Cumhuriyet Science Journal, 44(1), 19 - 27. 10.17776/csj.1180547
Chicago SAHIN INAN ZEYNEP DENIZ,Akın Polat Zübeyda,HAMUTOGLU RASIM The Effect of Two Different Botulinum Neurotoxin A On The Cortical Neuron Cells In Terms of Apoptosis and MMP 2, MMP 7, and MMP9 Localizations. Cumhuriyet Science Journal 44, no.1 (2023): 19 - 27. 10.17776/csj.1180547
MLA SAHIN INAN ZEYNEP DENIZ,Akın Polat Zübeyda,HAMUTOGLU RASIM The Effect of Two Different Botulinum Neurotoxin A On The Cortical Neuron Cells In Terms of Apoptosis and MMP 2, MMP 7, and MMP9 Localizations. Cumhuriyet Science Journal, vol.44, no.1, 2023, ss.19 - 27. 10.17776/csj.1180547
AMA SAHIN INAN Z,Akın Polat Z,HAMUTOGLU R The Effect of Two Different Botulinum Neurotoxin A On The Cortical Neuron Cells In Terms of Apoptosis and MMP 2, MMP 7, and MMP9 Localizations. Cumhuriyet Science Journal. 2023; 44(1): 19 - 27. 10.17776/csj.1180547
Vancouver SAHIN INAN Z,Akın Polat Z,HAMUTOGLU R The Effect of Two Different Botulinum Neurotoxin A On The Cortical Neuron Cells In Terms of Apoptosis and MMP 2, MMP 7, and MMP9 Localizations. Cumhuriyet Science Journal. 2023; 44(1): 19 - 27. 10.17776/csj.1180547
IEEE SAHIN INAN Z,Akın Polat Z,HAMUTOGLU R "The Effect of Two Different Botulinum Neurotoxin A On The Cortical Neuron Cells In Terms of Apoptosis and MMP 2, MMP 7, and MMP9 Localizations." Cumhuriyet Science Journal, 44, ss.19 - 27, 2023. 10.17776/csj.1180547
ISNAD SAHIN INAN, ZEYNEP DENIZ vd. "The Effect of Two Different Botulinum Neurotoxin A On The Cortical Neuron Cells In Terms of Apoptosis and MMP 2, MMP 7, and MMP9 Localizations". Cumhuriyet Science Journal 44/1 (2023), 19-27. https://doi.org/10.17776/csj.1180547
Sayfa Oluşturulma Tarihi
24-04-2024 18:50

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