This study aims to investigate the protective effects of baicalin (BAI) in rats exposed to cypermethrin (CYP). A total of 40 Wistar Albino male rats, 10 in each group, aged 6 to 8 weeks and weighing 180 to 220 g, were utilized for this experiment. The groups were determined, respectively, control, BAI (50 mg kg-1), CYP (25 mg kg-1), and CYP (25 mg kg-1) + BAI (50 mg kg-1). At the end of the 21-day trial period, blood samples were collected from rats by cardiac puncture into test tubes with anticoagulant and non-anticoagulant properties under intraperitoneal ketamine and xylazine anesthesia. Afterward, the rats were sacrificed by cervical dislocation, and the liver, kidney, brain, testis, heart and lung tissues were removed. Glutathione, NO, and MDA levels and GPx, GR, GST, SOD, and CAT enzyme activities in tissue and blood (plasma and erythrocyte) samples were measured on a microplate reader by a spectrophotometric method, whereas LDH, AST, ALT, ALP, triglyceride, cholesterol, albumin, total protein, BUN, uric acid and creatinine levels/activities in serum were measured on a Roche Cobas autoanalyzer. At the end of the experiment, it was determined that the parameters of the BAI group were similar to those of the control group (P>0.05). When the CYP- exposed group was compared to the control group, it was discovered that there was a statistically considerable de- crease in tissue GSH levels as well as GPx, GR, GST, SOD, and CAT enzyme activities (P<0.05). A significant in- crease was recorded in MDA and NO levels (P<0.05). Additionally, while serum total protein and albumin levels de- creased, AST, ALT, ALP, LDH, cholesterol, triglyceride, BUN, uric acid, and creatinine levels/activities increased (P<0.05). The effects of CYP were partially or completely ameliorated in the CYP and BAI co-administered group. Consequently, it was revealed that BAI may have a protective effect against CYP-induced oxidative damage in rats by suppressing oxidative stress and promoting antioxidant system activity.