In this study, the determination of glucose in the fluid was made by monitoring the redox reactions occurring on the biosensor integrated into a microfluidic system by cyclic voltammetry method. Comsol Multiphysics v3.5a was used for the design and finite element analysis of the microchannel integrated biosensor. In this context, an electrode was placed on a microchannel surface with a height of 300 µm and a length of 1500 µm. Mathematical modeling was primarily performed without fluid flow through the microchannel. A cyclic voltammetry-based microfluidic system was designed by continuing the analyzes by defining a liquid inlet to the microchannel inlet under laminar flow conditions. The efficiency of the biosensor was tested at different glucose values such as 0.25mM, 0.5mM, 1mM, 2mM, 4mM and 8mM. In order to determine the effect of the flow rate of the laminar flow defined at the entrance of the microchannel on the response from the biosensor, analyzes were made at different flow rates such as 25 µl/min, 50 µl/min, 100 µl/min and 200 µl/min. In order to examine the effect of electrode length on the response from cyclic voltammetry, the electrode lengths were changed to 100µm, 200µm and 300µm. In this study, it was observed that the maximum current obtained from cyclic voltammetry increased in the presence of laminar flow. Simulations have shown that increasing the fluid flow rate increases the efficiency of the biosensor. As a result, the feasibility of glucose determination with a cyclic voltammetry-based microfluidic system has been demonstrated with this study.