Proteomic changes during boron tolerance in barley (Hordeum vulgare) and the role of vacuolar proton-translocating ATPase subunit E

KARAKAYA, HÜSEYİN ÇAĞLAR; Koc, Ahmet; ATİK, Ahmet Emin; akinci, ersin; BOZDAĞ, Gönensin Ozan; KAYA, Alaattin; YALÇIN, TALAT

Proteomic changes during boron tolerance in barley (Hordeum vulgare) and the role of vacuolar proton-translocating ATPase subunit E

Ahmet Emin ATİK, (İzmir Yüksek Teknoloji Enstitüsü, Mühendislik Fakültesi, Kimya Mühendisliği Bölümü, İzmir, Türkiye)

Gönensin Ozan BOZDAĞ, (İzmir Yüksek Teknoloji Enstitüsü, Fen Fakültesi, Moleküler Biyoloji ve Genetik Bölümü, İzmir, Türkiye)

Ersin AKINCI, (İzmir Yüksek Teknoloji Enstitüsü, Fen Fakültesi, Moleküler Biyoloji ve Genetik Bölümü, İzmir, Türkiye)

Alaattin KAYA, (İzmir Yüksek Teknoloji Enstitüsü, Fen Fakültesi, Moleküler Biyoloji ve Genetik Bölümü, İzmir, Türkiye)

Ahmet KOÇ, (İzmir Yüksek Teknoloji Enstitüsü, Fen Fakültesi, Moleküler Biyoloji ve Genetik Bölümü, İzmir, Türkiye)

Talat YALÇIN, (İzmir Yüksek Teknoloji Enstitüsü, Mühendislik Fakültesi, Kimya Mühendisliği Bölümü, İzmir, Türkiye)

Hüseyin Çağlar KARAKAYA (İzmir Yüksek Teknoloji Enstitüsü, Fen Fakültesi, Moleküler Biyoloji ve Genetik Bölümü, İzmir, Türkiye)

Turkish Journal of Botany

4 0

Yıl: 2011 Cilt: 35 Sayı: 4 Sayfa Aralığı: 379 - 388 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Proteomic changes during boron tolerance in barley (Hordeum vulgare) and the role of vacuolar proton-translocating ATPase subunit E

Öz:

Bor, bitkiler ve hayvanlar için gerekli olan mikrolementlerdendir. Ancak yüksek konsantrasyonlarda toksik etki göstermektedir. Bu çalışmada bora dayanıklı Anadolu arpa (Hordeum vulgare) genotipindeki bor tolerans mekanizmasının anlaşılması amaçlanmıştır. Kontrol ve bor stresi uygulanmış ve Anadolu genotipi arasındaki farklı seviyede ekspres olan proteinleri taramak için 2 boyutlu elektroforez tekniği uygulanmıştır. İki boyutlu elektroforez deneyi sonucu ekspresyon seviyesi artan yedi protein bulunmuştur: ribuloz 1,5-bifosfat karboksilaz/oksijenaz büyük zincir, thaumatin benzeri protein TLP5, patojenaz benzeri protein PR5, RNaz S benzeri protein, PSI tipi III a/bbağlayıcı protein, ışık toplayan kompleks protein I LHC I ve vakuol proton translokasyon ATPaz alt birim E. V-ATPaz alt birim E geni tarafından kodlanan proteinin seviyesinin bor uygulaması ile artmasına rağmen, genin transkript seviyesi azalmıştır. Arpa V-ATPaz alt birim E geninin maya hücrelerinde ifadenlenmesi maya hücrelerine bora karşı dayanıklılık sağlamıştır. Bu sonuçlar, V-ATPaz alt birim E geninin mayada fonksiyonel olduğunu ve maya hücrelerine, toksik bor seviyelerine karşı dayanıklılık sağladığını ve de arpada bor toleransında bir rolü olabileceğini göstermektedir.

Anahtar Kelime: bor proteom analizi Hordeum vulgare stres faktörleri Saccharomyces cerevisiae tolerans adenozin trifosfataz toksisite

Konular: Biyoloji

Boron toleransı sırasında arpada (Hordeum vulgare) görülen proteomik değişimler ve vakuol proton translokasyon ATPaz alt birim E’nin rolü

Öz:

Boron is an essential micronutrient for plants and animals; however, it can be toxic when present at high concentrations. Th e purpose of this study was to understand the mechanisms of boron tolerance in the Turkish barley (Hordeum vulgare) Anadolu cultivar. For this purpose, 2-dimensional electrophoresis (2-DE) was used to screen diff erentially expressed proteins for both control and boron-stressed Anadolu barley genotypes. Seven proteins were revealed by 2-DE: 1) ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCo large chain), 2) TLP5, a thaumatin-like protein, 3) PR5, a basic pathogenesis-related protein, 4) a RNase S-like protein, 5) a PSI type III chlorophyll a/b-binding protein, 6) a light-harvesting complex I LHC I, and 7) the vacuolar proton-translocating ATPase subunit E protein. Th ese were found to be upregulated in response to boron treatment. Even though the protein encoded by the V-ATPase subunit E gene was overexpressed, its transcript level was downregulated by boron treatment. Heterologous expression of the barley V-ATPase subunit E gene in yeast provided boron resistance to yeast cells. Th ese results indicated that the V-ATPase subunit E gene was functional and conferred tolerance to toxic boron levels in yeast and might play a role in the overall boron tolerance of barley.

Anahtar Kelime: Saccharomyces cerevisiae tolerance adenosinetriphosphatase toxicity boron proteomics Hordeum vulgare stress factors

Konular: Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	ATİK A, BOZDAĞ G, akinci e, KAYA A, Koc A, YALÇIN T, KARAKAYA H (2011). Proteomic changes during boron tolerance in barley (Hordeum vulgare) and the role of vacuolar proton-translocating ATPase subunit E. , 379 - 388.
Chicago	ATİK Ahmet Emin,BOZDAĞ Gönensin Ozan,akinci ersin,KAYA Alaattin,Koc Ahmet,YALÇIN TALAT,KARAKAYA HÜSEYİN ÇAĞLAR Proteomic changes during boron tolerance in barley (Hordeum vulgare) and the role of vacuolar proton-translocating ATPase subunit E. (2011): 379 - 388.
MLA	ATİK Ahmet Emin,BOZDAĞ Gönensin Ozan,akinci ersin,KAYA Alaattin,Koc Ahmet,YALÇIN TALAT,KARAKAYA HÜSEYİN ÇAĞLAR Proteomic changes during boron tolerance in barley (Hordeum vulgare) and the role of vacuolar proton-translocating ATPase subunit E. , 2011, ss.379 - 388.
AMA	ATİK A,BOZDAĞ G,akinci e,KAYA A,Koc A,YALÇIN T,KARAKAYA H Proteomic changes during boron tolerance in barley (Hordeum vulgare) and the role of vacuolar proton-translocating ATPase subunit E. . 2011; 379 - 388.
Vancouver	ATİK A,BOZDAĞ G,akinci e,KAYA A,Koc A,YALÇIN T,KARAKAYA H Proteomic changes during boron tolerance in barley (Hordeum vulgare) and the role of vacuolar proton-translocating ATPase subunit E. . 2011; 379 - 388.
IEEE	ATİK A,BOZDAĞ G,akinci e,KAYA A,Koc A,YALÇIN T,KARAKAYA H "Proteomic changes during boron tolerance in barley (Hordeum vulgare) and the role of vacuolar proton-translocating ATPase subunit E." , ss.379 - 388, 2011.
ISNAD	ATİK, Ahmet Emin vd. "Proteomic changes during boron tolerance in barley (Hordeum vulgare) and the role of vacuolar proton-translocating ATPase subunit E". (2011), 379-388.

APA	ATİK A, BOZDAĞ G, akinci e, KAYA A, Koc A, YALÇIN T, KARAKAYA H (2011). Proteomic changes during boron tolerance in barley (Hordeum vulgare) and the role of vacuolar proton-translocating ATPase subunit E. Turkish Journal of Botany, 35(4), 379 - 388.
Chicago	ATİK Ahmet Emin,BOZDAĞ Gönensin Ozan,akinci ersin,KAYA Alaattin,Koc Ahmet,YALÇIN TALAT,KARAKAYA HÜSEYİN ÇAĞLAR Proteomic changes during boron tolerance in barley (Hordeum vulgare) and the role of vacuolar proton-translocating ATPase subunit E. Turkish Journal of Botany 35, no.4 (2011): 379 - 388.
MLA	ATİK Ahmet Emin,BOZDAĞ Gönensin Ozan,akinci ersin,KAYA Alaattin,Koc Ahmet,YALÇIN TALAT,KARAKAYA HÜSEYİN ÇAĞLAR Proteomic changes during boron tolerance in barley (Hordeum vulgare) and the role of vacuolar proton-translocating ATPase subunit E. Turkish Journal of Botany, vol.35, no.4, 2011, ss.379 - 388.
AMA	ATİK A,BOZDAĞ G,akinci e,KAYA A,Koc A,YALÇIN T,KARAKAYA H Proteomic changes during boron tolerance in barley (Hordeum vulgare) and the role of vacuolar proton-translocating ATPase subunit E. Turkish Journal of Botany. 2011; 35(4): 379 - 388.
Vancouver	ATİK A,BOZDAĞ G,akinci e,KAYA A,Koc A,YALÇIN T,KARAKAYA H Proteomic changes during boron tolerance in barley (Hordeum vulgare) and the role of vacuolar proton-translocating ATPase subunit E. Turkish Journal of Botany. 2011; 35(4): 379 - 388.
IEEE	ATİK A,BOZDAĞ G,akinci e,KAYA A,Koc A,YALÇIN T,KARAKAYA H "Proteomic changes during boron tolerance in barley (Hordeum vulgare) and the role of vacuolar proton-translocating ATPase subunit E." Turkish Journal of Botany, 35, ss.379 - 388, 2011.
ISNAD	ATİK, Ahmet Emin vd. "Proteomic changes during boron tolerance in barley (Hordeum vulgare) and the role of vacuolar proton-translocating ATPase subunit E". Turkish Journal of Botany 35/4 (2011), 379-388.