Yıl: 2011 Cilt: 35 Sayı: 4 Sayfa Aralığı: 379 - 388 Metin Dili: Türkçe

Proteomic changes during boron tolerance in barley (Hordeum vulgare) and the role of vacuolar proton-translocating ATPase subunit E

Öz:
Bor, bitkiler ve hayvanlar için gerekli olan mikrolementlerdendir. Ancak yüksek konsantrasyonlarda toksik etki göstermektedir. Bu çalışmada bora dayanıklı Anadolu arpa (Hordeum vulgare) genotipindeki bor tolerans mekanizmasının anlaşılması amaçlanmıştır. Kontrol ve bor stresi uygulanmış ve Anadolu genotipi arasındaki farklı seviyede ekspres olan proteinleri taramak için 2 boyutlu elektroforez tekniği uygulanmıştır. İki boyutlu elektroforez deneyi sonucu ekspresyon seviyesi artan yedi protein bulunmuştur: ribuloz 1,5-bifosfat karboksilaz/oksijenaz büyük zincir, thaumatin benzeri protein TLP5, patojenaz benzeri protein PR5, RNaz S benzeri protein, PSI tipi III a/bbağlayıcı protein, ışık toplayan kompleks protein I LHC I ve vakuol proton translokasyon ATPaz alt birim E. V-ATPaz alt birim E geni tarafından kodlanan proteinin seviyesinin bor uygulaması ile artmasına rağmen, genin transkript seviyesi azalmıştır. Arpa V-ATPaz alt birim E geninin maya hücrelerinde ifadenlenmesi maya hücrelerine bora karşı dayanıklılık sağlamıştır. Bu sonuçlar, V-ATPaz alt birim E geninin mayada fonksiyonel olduğunu ve maya hücrelerine, toksik bor seviyelerine karşı dayanıklılık sağladığını ve de arpada bor toleransında bir rolü olabileceğini göstermektedir.
Anahtar Kelime: bor Saccharomyces cerevisiae Hordeum vulgare proteom analizi stres faktörleri adenozin trifosfataz tolerans toksisite

Boron toleransı sırasında arpada (Hordeum vulgare) görülen proteomik değişimler ve vakuol proton translokasyon ATPaz alt birim E’nin rolü

Öz:
Boron is an essential micronutrient for plants and animals; however, it can be toxic when present at high concentrations. Th e purpose of this study was to understand the mechanisms of boron tolerance in the Turkish barley (Hordeum vulgare) Anadolu cultivar. For this purpose, 2-dimensional electrophoresis (2-DE) was used to screen diff erentially expressed proteins for both control and boron-stressed Anadolu barley genotypes. Seven proteins were revealed by 2-DE: 1) ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCo large chain), 2) TLP5, a thaumatin-like protein, 3) PR5, a basic pathogenesis-related protein, 4) a RNase S-like protein, 5) a PSI type III chlorophyll a/b-binding protein, 6) a light-harvesting complex I LHC I, and 7) the vacuolar proton-translocating ATPase subunit E protein. Th ese were found to be upregulated in response to boron treatment. Even though the protein encoded by the V-ATPase subunit E gene was overexpressed, its transcript level was downregulated by boron treatment. Heterologous expression of the barley V-ATPase subunit E gene in yeast provided boron resistance to yeast cells. Th ese results indicated that the V-ATPase subunit E gene was functional and conferred tolerance to toxic boron levels in yeast and might play a role in the overall boron tolerance of barley.
Anahtar Kelime: proteomics boron adenosinetriphosphatase Hordeum vulgare Saccharomyces cerevisiae tolerance stress factors toxicity

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA ATİK A, BOZDAĞ G, AKINCI E, KAYA A, KOÇ A, YALÇIN t, KARAKAYA H (2011). Proteomic changes during boron tolerance in barley (Hordeum vulgare) and the role of vacuolar proton-translocating ATPase subunit E. Turkish Journal of Botany, 35(4), 379 - 388.
Chicago ATİK Ahmet Emin,BOZDAĞ Gönensin Ozan,AKINCI ERSİN,KAYA Alaattin,KOÇ AHMET,YALÇIN talat,KARAKAYA HÜSEYİN ÇAĞLAR Proteomic changes during boron tolerance in barley (Hordeum vulgare) and the role of vacuolar proton-translocating ATPase subunit E. Turkish Journal of Botany 35, no.4 (2011): 379 - 388.
MLA ATİK Ahmet Emin,BOZDAĞ Gönensin Ozan,AKINCI ERSİN,KAYA Alaattin,KOÇ AHMET,YALÇIN talat,KARAKAYA HÜSEYİN ÇAĞLAR Proteomic changes during boron tolerance in barley (Hordeum vulgare) and the role of vacuolar proton-translocating ATPase subunit E. Turkish Journal of Botany, vol.35, no.4, 2011, ss.379 - 388.
AMA ATİK A,BOZDAĞ G,AKINCI E,KAYA A,KOÇ A,YALÇIN t,KARAKAYA H Proteomic changes during boron tolerance in barley (Hordeum vulgare) and the role of vacuolar proton-translocating ATPase subunit E. Turkish Journal of Botany. 2011; 35(4): 379 - 388.
Vancouver ATİK A,BOZDAĞ G,AKINCI E,KAYA A,KOÇ A,YALÇIN t,KARAKAYA H Proteomic changes during boron tolerance in barley (Hordeum vulgare) and the role of vacuolar proton-translocating ATPase subunit E. Turkish Journal of Botany. 2011; 35(4): 379 - 388.
IEEE ATİK A,BOZDAĞ G,AKINCI E,KAYA A,KOÇ A,YALÇIN t,KARAKAYA H "Proteomic changes during boron tolerance in barley (Hordeum vulgare) and the role of vacuolar proton-translocating ATPase subunit E." Turkish Journal of Botany, 35, ss.379 - 388, 2011.
ISNAD ATİK, Ahmet Emin vd. "Proteomic changes during boron tolerance in barley (Hordeum vulgare) and the role of vacuolar proton-translocating ATPase subunit E". Turkish Journal of Botany 35/4 (2011), 379-388.