SCREENING OF MICROFUNGI ISOLATED FROM ACIGÖL, TÜRKİYE FOR HYDROLYTIC ENZYMES, BIOACTIVE METABOLITES AND SILVER NANOPARTICLE PRODUCTION
Yıl: 2023 Cilt: 24 Sayı: 1 Sayfa Aralığı: 51 - 62 Metin Dili: İngilizce DOI: 10.23902/trkjnat.1190972 İndeks Tarihi: 09-05-2023
SCREENING OF MICROFUNGI ISOLATED FROM ACIGÖL, TÜRKİYE FOR HYDROLYTIC ENZYMES, BIOACTIVE METABOLITES AND SILVER NANOPARTICLE PRODUCTION
Öz: Haloalkalitolerant fungi can survive in environments with high salt concentrations and pH values. The bioactive compounds produced under stressful conditions have potential biotechnological applications. In this study, 52 microfungi isolated from Acıgöl Lake in Türkiye, offering polyextreme conditions, were screened for some biotechnological properties. The antimicrobial and antioxidant activities of the isolates were determined using the agar diffusion and the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging methods, respectively. Enzyme activities were determined by various methods using the agar diffusion technique. Synthesis of silver nanoparticles (AgNPs) was carried out using cell-free filtrate of microfungi. 40% of the isolates showed antimicrobial activity against at least one of Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 29213 and Candida albicans ATCC 90028 used as the test microorganisms. Penicillium dipodomyicola showed the highest antibacterial activity against E. coli and S. aureus, while P. brevicompactum showed the highest activity against C. albicans. Penicillium dipodomyicola and P. bilaiae were found to have free radical scavenging activity of a level (90% and above) that can compete with positive control. All of the isolates with amylase activity belonged to Aspergillus and Penicillium and the most prominent three of them were A. ochraceous, A. flavus and P. brevicompactum. 55% of the isolates showed proteolytic activity, among which A. alliaceus had the highest activity. Almost all isolates (92%) showed lipolytic activity. Aspergillus amstelodami, P. sizovae and P. solitum had a significant level of lipolytic activity. 35% of the isolates showed cellulolytic activity with highest values Cladosporium pseudocladosporioides, P. dipodomyicola and P. bilaiae. Eight of the isolates carried out AgNP synthesis within 24 h. When all the results were evaluated, Aspergillus amstelodami, A. ochraceus, Penicillium dipodomyicola, and P. brevicompactum appeared to have the potential to serve in different industrial areas.
Anahtar Kelime: -
Öz: Haloalkalitolerant funguslar, tuz konsantrasyonunun ve pH değerinin yüksek olduğu ortamlarda yaşayabilirler. Stresli koşullar altında üretilen biyoaktif bileşiklerin potansiyel biyoteknolojik uygulamaları vardır. Bu çalışmada, poliekstrem koşullara sahip Acıgöl/Türkiye'den izole edilen 52 mikrofungus, bazı biyoteknolojik özellikler açısından taranmıştır. Antimikrobiyal ve antioksidan aktiviteler sırasıyla agar difüzyon ve 2,2-difenil- 1-pikrilhidrazil (DPPH) radikal süpürücü etki yöntemi kullanılarak belirlenmiştir. Enzim aktiviteleri, agar difüzyon tekniğinin kullanıldığı çeşitli yöntemlerle belirlenmiştir. Gümüş nanopartikül (AgNP) sentezi, hücre içermeyen mikrofungus filtratı kullanılarak gerçekleştirilmiştir. İzolatların %40'ı test mikroorganizmalarından en az birine karşı antimikrobiyal aktivite göstermiştir. Penicillium dipodomyicola, E. coli ve S. aureus’a karşı en yüksek antibakteriyel aktiviteyi gösterirken, P. brevicompactum Candida albicans'a karşı en yüksek aktiviteyi göstermiştir. Penicillium dipodomyicola ve P. bilaiae'nin pozitif kontrol ile rekabet edebilecek düzeyde (%90 ve üzeri) serbest radikal süpürücü aktiviteye sahip olduğu belirlenmiştir. Amilaz aktivitesine sahip izolatların tamamı Aspergillus ve Penicillium cinslerine ait olup, A. ochraceous, A. flavus ve P. brevicompactum en yüksek aktiviteye sahip türler olarak belirlenmiştir. İzolatların %55'i proteolitik aktivite gösterirken, A. alliaceus en yüksek aktiviteye sahip izolat olmuştur. Hemen hemen tüm izolatlar (%92) lipolitik aktivite göstermiştir. Özellikle A. amstelodami, P. sizovae ve P. solitum izolatlarının önemli düzeyde lipolitik aktiviteye sahip olduğu belirlenmiştir. İzolatların %35'i selülolitik aktivite göstermiş ve Cladosporium pseudocladosporioides, P. dipodomyicola ve P. bilaiae izolatlarında yüksek aktivite gözlenmiştir. İzolatların sekizi 24 saat içinde AgNP sentezini gerçekleştirmiştir. Tüm sonuçlar değerlendirildiğinde Aspergillus amstelodami, A. ochraceus, Penicillium dipodomyicola ve P. brevicompactum gibi izolatların farklı endüstriyel alanlarda hizmet verme potansiyeline sahip olduğu belirlenmiştir.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | İrdem E, İlhan S, Özbiçen E, USAKBEK KYZY L, Tunca G, Ocak E, ZORLUER N, ÇİĞDEM U, AYVA F, Demirel R (2023). SCREENING OF MICROFUNGI ISOLATED FROM ACIGÖL, TÜRKİYE FOR HYDROLYTIC ENZYMES, BIOACTIVE METABOLITES AND SILVER NANOPARTICLE PRODUCTION. , 51 - 62. 10.23902/trkjnat.1190972 |
| Chicago | İrdem Emine,İlhan Semra,Özbiçen Ercan,USAKBEK KYZY LIRA,Tunca Gamze,Ocak Esma,ZORLUER Niyazi Can,ÇİĞDEM UĞUR,AYVA Fatma,Demirel Rasime SCREENING OF MICROFUNGI ISOLATED FROM ACIGÖL, TÜRKİYE FOR HYDROLYTIC ENZYMES, BIOACTIVE METABOLITES AND SILVER NANOPARTICLE PRODUCTION. (2023): 51 - 62. 10.23902/trkjnat.1190972 |
| MLA | İrdem Emine,İlhan Semra,Özbiçen Ercan,USAKBEK KYZY LIRA,Tunca Gamze,Ocak Esma,ZORLUER Niyazi Can,ÇİĞDEM UĞUR,AYVA Fatma,Demirel Rasime SCREENING OF MICROFUNGI ISOLATED FROM ACIGÖL, TÜRKİYE FOR HYDROLYTIC ENZYMES, BIOACTIVE METABOLITES AND SILVER NANOPARTICLE PRODUCTION. , 2023, ss.51 - 62. 10.23902/trkjnat.1190972 |
| AMA | İrdem E,İlhan S,Özbiçen E,USAKBEK KYZY L,Tunca G,Ocak E,ZORLUER N,ÇİĞDEM U,AYVA F,Demirel R SCREENING OF MICROFUNGI ISOLATED FROM ACIGÖL, TÜRKİYE FOR HYDROLYTIC ENZYMES, BIOACTIVE METABOLITES AND SILVER NANOPARTICLE PRODUCTION. . 2023; 51 - 62. 10.23902/trkjnat.1190972 |
| Vancouver | İrdem E,İlhan S,Özbiçen E,USAKBEK KYZY L,Tunca G,Ocak E,ZORLUER N,ÇİĞDEM U,AYVA F,Demirel R SCREENING OF MICROFUNGI ISOLATED FROM ACIGÖL, TÜRKİYE FOR HYDROLYTIC ENZYMES, BIOACTIVE METABOLITES AND SILVER NANOPARTICLE PRODUCTION. . 2023; 51 - 62. 10.23902/trkjnat.1190972 |
| IEEE | İrdem E,İlhan S,Özbiçen E,USAKBEK KYZY L,Tunca G,Ocak E,ZORLUER N,ÇİĞDEM U,AYVA F,Demirel R "SCREENING OF MICROFUNGI ISOLATED FROM ACIGÖL, TÜRKİYE FOR HYDROLYTIC ENZYMES, BIOACTIVE METABOLITES AND SILVER NANOPARTICLE PRODUCTION." , ss.51 - 62, 2023. 10.23902/trkjnat.1190972 |
| ISNAD | İrdem, Emine vd. "SCREENING OF MICROFUNGI ISOLATED FROM ACIGÖL, TÜRKİYE FOR HYDROLYTIC ENZYMES, BIOACTIVE METABOLITES AND SILVER NANOPARTICLE PRODUCTION". (2023), 51-62. https://doi.org/10.23902/trkjnat.1190972 |
| APA | İrdem E, İlhan S, Özbiçen E, USAKBEK KYZY L, Tunca G, Ocak E, ZORLUER N, ÇİĞDEM U, AYVA F, Demirel R (2023). SCREENING OF MICROFUNGI ISOLATED FROM ACIGÖL, TÜRKİYE FOR HYDROLYTIC ENZYMES, BIOACTIVE METABOLITES AND SILVER NANOPARTICLE PRODUCTION. Trakya University Journal of Natural Sciences, 24(1), 51 - 62. 10.23902/trkjnat.1190972 |
| Chicago | İrdem Emine,İlhan Semra,Özbiçen Ercan,USAKBEK KYZY LIRA,Tunca Gamze,Ocak Esma,ZORLUER Niyazi Can,ÇİĞDEM UĞUR,AYVA Fatma,Demirel Rasime SCREENING OF MICROFUNGI ISOLATED FROM ACIGÖL, TÜRKİYE FOR HYDROLYTIC ENZYMES, BIOACTIVE METABOLITES AND SILVER NANOPARTICLE PRODUCTION. Trakya University Journal of Natural Sciences 24, no.1 (2023): 51 - 62. 10.23902/trkjnat.1190972 |
| MLA | İrdem Emine,İlhan Semra,Özbiçen Ercan,USAKBEK KYZY LIRA,Tunca Gamze,Ocak Esma,ZORLUER Niyazi Can,ÇİĞDEM UĞUR,AYVA Fatma,Demirel Rasime SCREENING OF MICROFUNGI ISOLATED FROM ACIGÖL, TÜRKİYE FOR HYDROLYTIC ENZYMES, BIOACTIVE METABOLITES AND SILVER NANOPARTICLE PRODUCTION. Trakya University Journal of Natural Sciences, vol.24, no.1, 2023, ss.51 - 62. 10.23902/trkjnat.1190972 |
| AMA | İrdem E,İlhan S,Özbiçen E,USAKBEK KYZY L,Tunca G,Ocak E,ZORLUER N,ÇİĞDEM U,AYVA F,Demirel R SCREENING OF MICROFUNGI ISOLATED FROM ACIGÖL, TÜRKİYE FOR HYDROLYTIC ENZYMES, BIOACTIVE METABOLITES AND SILVER NANOPARTICLE PRODUCTION. Trakya University Journal of Natural Sciences. 2023; 24(1): 51 - 62. 10.23902/trkjnat.1190972 |
| Vancouver | İrdem E,İlhan S,Özbiçen E,USAKBEK KYZY L,Tunca G,Ocak E,ZORLUER N,ÇİĞDEM U,AYVA F,Demirel R SCREENING OF MICROFUNGI ISOLATED FROM ACIGÖL, TÜRKİYE FOR HYDROLYTIC ENZYMES, BIOACTIVE METABOLITES AND SILVER NANOPARTICLE PRODUCTION. Trakya University Journal of Natural Sciences. 2023; 24(1): 51 - 62. 10.23902/trkjnat.1190972 |
| IEEE | İrdem E,İlhan S,Özbiçen E,USAKBEK KYZY L,Tunca G,Ocak E,ZORLUER N,ÇİĞDEM U,AYVA F,Demirel R "SCREENING OF MICROFUNGI ISOLATED FROM ACIGÖL, TÜRKİYE FOR HYDROLYTIC ENZYMES, BIOACTIVE METABOLITES AND SILVER NANOPARTICLE PRODUCTION." Trakya University Journal of Natural Sciences, 24, ss.51 - 62, 2023. 10.23902/trkjnat.1190972 |
| ISNAD | İrdem, Emine vd. "SCREENING OF MICROFUNGI ISOLATED FROM ACIGÖL, TÜRKİYE FOR HYDROLYTIC ENZYMES, BIOACTIVE METABOLITES AND SILVER NANOPARTICLE PRODUCTION". Trakya University Journal of Natural Sciences 24/1 (2023), 51-62. https://doi.org/10.23902/trkjnat.1190972 |
