Objective: Infectious gastroenteritis is one of the most important causes of morbidity and mortality, especially in children. Rotavirus and enteric adenoviruses are important viral agents of infectious gastroenteritis. In our study, we aimed to determine the positivity of rotavirus and adenovirus and the gender, age and seasonal distribution of the causative agents in stool samples sent to the laboratory with a preliminary diagnosis of acute gastroenteritis.Material and Methods: Stool samples sent from various clinics to the microbiology laboratory with the pre-diagnosis of acute gastroenteritis between July 2015 and June 2019 were examined for the presence of rotavirus and adenovirus. The Simple/StickRota Adeno (Operon, Spain) kit working with the qualitative immunochromatographic method was used to analyze the stool samples. We reviewed the study data retrospectively. Statistical evaluations were made according to the “chi square test”.Results: Rotavirus positivity was found in 472 (8.91%) of 5294 samples included in the study, and adenovirus positivity was found in 237 (4.4%) samples. Both viruses were detected in 99 (1.07%) samples. Rotavirus positivity was 104 (22%) between 13-24 months and 116(24.5%) between 2-5 years of age; adenovirus positivity was found to be 38 (16%) between the ages of 2-5 and 85 (35.8%) over the age of 18. Seasonally, 179 (37.9%) of rotavirus infections were spring, 153 (32.5%) were winter, 65 (27.4%) of adenovirus infections were detected in winter and 61 (25.8%) in summer.Conclusion: Rotavirus and adenovirus, which are viral gastroenteritis agents, can be easily detected by antigen tests in stool. Knowing the rotavirus and adenovirus positivity rates, age, gender and seasonal distributions will contribute to regional and country-wide data.