Response of Different Safflower Genotypes to Anther Culture
Yıl: 2023 Cilt: 26 Sayı: 2 Sayfa Aralığı: 293 - 298 Metin Dili: İngilizce DOI: 10.18016/ksutarimdoga.vi.1084134 İndeks Tarihi: 04-05-2023
Response of Different Safflower Genotypes to Anther Culture
Öz: In this study; the responses of 12 safflower genotypes to anther culture were determined in MS medium containing increasing doses of BAP (0, 0.5, 1, and 2 mg L-1) and NAA (0, 0.1, and 0.5 mg L-1). Donor plants were grown under field conditions and the main capitulas were collected in early or late uninucleate stage. A pre-treatment was applied to their capitules at +4 °C for 3-4 days. Only cvs. Balcı and Linas produced regenerable callus among these genotypes. Although regenerable callus rate was higher in Balcı than Linas, shoot regeneration was achieved only in Linas with 0.5 or 1 mg L-1 of BAP + 0.1 mg L-1 of NAA combinations. In total, 8 shoots of Linas were moved to ½ MS medium consisting of 0.1 mg L-1 of NAA, but rooting of them could not be performed. In sum, among the 12 safflower genotypes, cvs. Balcı and Linas have higher anther culture regeneration potential; however, it is necessary to focus on shoot development and rooting on these cultivars in further experiments.
Anahtar Kelime: Farklı Aspir Genotiplerinin Anter Kültürüne Tepkisi
Öz: Bu çalışmada; 12 aspir genotipinin artan dozlarda BAP (0, 0.5, 1 ve 2 mg L-1) ve NAA (0, 0.1 ve 0.5 mg L-1) içeren MS ortamında anter kültürüne karşı tepkileri belirlenmiştir. Donör bitkiler tarla koşullarında yetiştirilmiş ve ana çiçek tablaları erken veya geç tek çekirdekli dönemde hasat edilmiştir. Ön işlem olarak +4 °C’de ve 3-4 gün süreyle soğuk muamelesi uygulanmıştır. Aspir genotiplerinden sadece Balcı ve Linas çeşitleri rejenere olabilir kallus üretmiştir. Balcı çeşidi daha yüksek oranda kallus oluşturmasına rağmen sadece Linas çeşidinde 0.5 veya 1 mg L-1 BAP + 0.1 mg L-1 NAA kombinasyonlarından sürgün rejenerasyonu gerçekleşmiştir. Toplamda Linas çeşidine ait 8 sürgün 0.1 mg L-1 NAA içeren ½ MS ortamına aktarılmış, ancak köklenme sağlanamamıştır. Sonuç olarak; 12 aspir genotipi arasında Balcı ve Linas çeşitlerinin anter kültürü rejenerasyon potansiyelinin daha yüksek olduğu belirlenmiştir. Bununla birlikte, bu çeşitler üzerinde gelecekte sürgün geliştirme ve köklendirme çalışmalarına odaklanılması gerekmektedir.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | D, AVCI S, Kaya M (2023). Response of Different Safflower Genotypes to Anther Culture. , 293 - 298. 10.18016/ksutarimdoga.vi.1084134 |
| Chicago | Dudu Büşra Nakas,AVCI Süleyman,Kaya Mehmet Demir Response of Different Safflower Genotypes to Anther Culture. (2023): 293 - 298. 10.18016/ksutarimdoga.vi.1084134 |
| MLA | Dudu Büşra Nakas,AVCI Süleyman,Kaya Mehmet Demir Response of Different Safflower Genotypes to Anther Culture. , 2023, ss.293 - 298. 10.18016/ksutarimdoga.vi.1084134 |
| AMA | D,AVCI S,Kaya M Response of Different Safflower Genotypes to Anther Culture. . 2023; 293 - 298. 10.18016/ksutarimdoga.vi.1084134 |
| Vancouver | D,AVCI S,Kaya M Response of Different Safflower Genotypes to Anther Culture. . 2023; 293 - 298. 10.18016/ksutarimdoga.vi.1084134 |
| IEEE | D,AVCI S,Kaya M "Response of Different Safflower Genotypes to Anther Culture." , ss.293 - 298, 2023. 10.18016/ksutarimdoga.vi.1084134 |
| ISNAD | , Dudu Büşra Nakas vd. "Response of Different Safflower Genotypes to Anther Culture". (2023), 293-298. https://doi.org/10.18016/ksutarimdoga.vi.1084134 |
| APA | D, AVCI S, Kaya M (2023). Response of Different Safflower Genotypes to Anther Culture. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 26(2), 293 - 298. 10.18016/ksutarimdoga.vi.1084134 |
| Chicago | Dudu Büşra Nakas,AVCI Süleyman,Kaya Mehmet Demir Response of Different Safflower Genotypes to Anther Culture. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 26, no.2 (2023): 293 - 298. 10.18016/ksutarimdoga.vi.1084134 |
| MLA | Dudu Büşra Nakas,AVCI Süleyman,Kaya Mehmet Demir Response of Different Safflower Genotypes to Anther Culture. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, vol.26, no.2, 2023, ss.293 - 298. 10.18016/ksutarimdoga.vi.1084134 |
| AMA | D,AVCI S,Kaya M Response of Different Safflower Genotypes to Anther Culture. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi. 2023; 26(2): 293 - 298. 10.18016/ksutarimdoga.vi.1084134 |
| Vancouver | D,AVCI S,Kaya M Response of Different Safflower Genotypes to Anther Culture. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi. 2023; 26(2): 293 - 298. 10.18016/ksutarimdoga.vi.1084134 |
| IEEE | D,AVCI S,Kaya M "Response of Different Safflower Genotypes to Anther Culture." Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 26, ss.293 - 298, 2023. 10.18016/ksutarimdoga.vi.1084134 |
| ISNAD | , Dudu Büşra Nakas vd. "Response of Different Safflower Genotypes to Anther Culture". Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 26/2 (2023), 293-298. https://doi.org/10.18016/ksutarimdoga.vi.1084134 |
