Efficacy of Topical Boric Acid in the Treatment of Experimental Pseudomonas Aeruginosa Keratitis in Rats
Yıl: 2023 Cilt: 30 Sayı: 1 Sayfa Aralığı: 60 - 65 Metin Dili: İngilizce DOI: 10.5505/vtd.2023.15425 İndeks Tarihi: 04-05-2023
Efficacy of Topical Boric Acid in the Treatment of Experimental Pseudomonas Aeruginosa Keratitis in Rats
Öz: Introduction: It was aimed to investigate the effect of topical boric acid on healing in the treatment of keratitis. Materials and Methods: Keratitis was created by randomly 32 of 56 healthy rats with inoculating pseudomonas aeruginosa (1x108 colony-forming units/ml 0.05 ml of solution containing pseudomonas eruginosa-PA-ATC27853) by creating epithelial defects in the corneas and randomly distributed to 4 groups. The remaining 24 healthy rats were randomly divided into 3 groups, so that; group 1 (control group), group 2 (with pseudomonas keratitis), group 3 (with pseudomonas keratitis and 4% boric acid treatment), group 4 (with pseudomonas keratitis and 8% boric acid treatment), group 5 (with pseudomonas keratitis and vancomycin+ ceftazidime given), group 6 (non-keratitis and only 4% boric acid applied), group 7 (non-keratitis and only 8% boric acid applied). At the end of the study, the ocular tissues of the rats were removed and examined histopathologically. Results: A clinically significant reduction in inflammation was detected in the groups using topical boric acid. Decreased TGF- β1 staining was detected in the groups in which keratitis + %4 and %8 boric acid was treated topically (p˂0,05). Compared to the infected group (group 2), 4% and 8% boric acid administered groups revealed mild/moderate corneal edema and thickening, degeneration of the corneal epithelium, and mild/moderate inflammation in the interstitial. Conclusion: Topical boric acid drops may be an important therapeutic factor in tissue healing and in the fight against inflammation in the treatment of keratitis.
Anahtar Kelime: Sıçanlarda Deneysel Pseudomonas Aeruginosa Keratiti Tedavisinde Topikal Borik Asitin Etkinliği
Öz: Amaç: Bu çalışmada keratit tedavisinde topikal borik asitin iyileşmeye etkisinin araştırılması amaçlanmıştır. Gereç ve Yöntem: 56 sağlıklı rattan rastgele 32 tanesinin kornealarında epitel defekti oluşturulup psödomonas aeruginosa (1x108 koloni oluşturan birim/ml pseudomonas eruginosa (PAATC27853) içeren solüsyondan 0.05 ml) inoküle edilerek keratit oluşturulmuş ve rastgele 4 gruba eşit olarak dağıtılmıştır. Geriye kalan 24 sağlıklı rat rastgele 3 gruba eşit olarak dağıtılmıştır. Böylece; grup 1 (kontrol grubu), grup 2 (psödomonas keratitli), grup 3 (psödomonas keratitli ve %4 borik asit tedavisi uygulanan), grup 4 (psödomonas keratitli ve %8 borik asit tedavisi uygulanan), grup 5 (psödomonas keratitli ve vankomisin+seftazidim uygulanan), grup 6 (keratit olmayan ve sadece %4 borik asit uygulanan), grup 7 (keratit olmayan ve sadece %8 borik asit uygulanan) olarak oluşturulmuştur. Çalışmanın sonunda sıçanların oküler dokuları çıkarılarak histopatolojik olarak incelenmiştir. Bulgular: Topikal borik asit (%4 ve %8) uygulanan gruplarda inflamasyonda anlamlı bir azalma saptanmıştır. Keratit + topikal %4 ve %8 borik asit uygulanan gruplarda TGF-β1 boyanmasında azalma saptanmıştır (p˂0,05). Şiddetli inflamasyon ve dokularda şiddetli dejenerasyon içeren keratitli grupla (grup 2) karşılaştırıldığında, %4 ve %8 borik asit uygulanan gruplarda yapılan incelemede hafif/orta düzeyde kornea ödemi ve korneal kalınlaşma, hafif/orta düzeyde kornea epitelinde dejenerasyon ve intertisyel dokularda hafif/orta derecede dejenerasyon ve inflamasyon saptanmıştır. Sonuç: Keratit tedavisinde topikal borik asit damlaları doku iyileşmesinde ve inflamasyonla mücadelede önemli bir terapötik faktör olabilir.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | ondas o, UTLU B, BAYRAKCEKEN K, yildirim S (2023). Efficacy of Topical Boric Acid in the Treatment of Experimental Pseudomonas Aeruginosa Keratitis in Rats. , 60 - 65. 10.5505/vtd.2023.15425 |
| Chicago | ondas osman,UTLU BAHADIR,BAYRAKCEKEN KEMAL,yildirim Serkan Efficacy of Topical Boric Acid in the Treatment of Experimental Pseudomonas Aeruginosa Keratitis in Rats. (2023): 60 - 65. 10.5505/vtd.2023.15425 |
| MLA | ondas osman,UTLU BAHADIR,BAYRAKCEKEN KEMAL,yildirim Serkan Efficacy of Topical Boric Acid in the Treatment of Experimental Pseudomonas Aeruginosa Keratitis in Rats. , 2023, ss.60 - 65. 10.5505/vtd.2023.15425 |
| AMA | ondas o,UTLU B,BAYRAKCEKEN K,yildirim S Efficacy of Topical Boric Acid in the Treatment of Experimental Pseudomonas Aeruginosa Keratitis in Rats. . 2023; 60 - 65. 10.5505/vtd.2023.15425 |
| Vancouver | ondas o,UTLU B,BAYRAKCEKEN K,yildirim S Efficacy of Topical Boric Acid in the Treatment of Experimental Pseudomonas Aeruginosa Keratitis in Rats. . 2023; 60 - 65. 10.5505/vtd.2023.15425 |
| IEEE | ondas o,UTLU B,BAYRAKCEKEN K,yildirim S "Efficacy of Topical Boric Acid in the Treatment of Experimental Pseudomonas Aeruginosa Keratitis in Rats." , ss.60 - 65, 2023. 10.5505/vtd.2023.15425 |
| ISNAD | ondas, osman vd. "Efficacy of Topical Boric Acid in the Treatment of Experimental Pseudomonas Aeruginosa Keratitis in Rats". (2023), 60-65. https://doi.org/10.5505/vtd.2023.15425 |
| APA | ondas o, UTLU B, BAYRAKCEKEN K, yildirim S (2023). Efficacy of Topical Boric Acid in the Treatment of Experimental Pseudomonas Aeruginosa Keratitis in Rats. Van Tıp Dergisi, 30(1), 60 - 65. 10.5505/vtd.2023.15425 |
| Chicago | ondas osman,UTLU BAHADIR,BAYRAKCEKEN KEMAL,yildirim Serkan Efficacy of Topical Boric Acid in the Treatment of Experimental Pseudomonas Aeruginosa Keratitis in Rats. Van Tıp Dergisi 30, no.1 (2023): 60 - 65. 10.5505/vtd.2023.15425 |
| MLA | ondas osman,UTLU BAHADIR,BAYRAKCEKEN KEMAL,yildirim Serkan Efficacy of Topical Boric Acid in the Treatment of Experimental Pseudomonas Aeruginosa Keratitis in Rats. Van Tıp Dergisi, vol.30, no.1, 2023, ss.60 - 65. 10.5505/vtd.2023.15425 |
| AMA | ondas o,UTLU B,BAYRAKCEKEN K,yildirim S Efficacy of Topical Boric Acid in the Treatment of Experimental Pseudomonas Aeruginosa Keratitis in Rats. Van Tıp Dergisi. 2023; 30(1): 60 - 65. 10.5505/vtd.2023.15425 |
| Vancouver | ondas o,UTLU B,BAYRAKCEKEN K,yildirim S Efficacy of Topical Boric Acid in the Treatment of Experimental Pseudomonas Aeruginosa Keratitis in Rats. Van Tıp Dergisi. 2023; 30(1): 60 - 65. 10.5505/vtd.2023.15425 |
| IEEE | ondas o,UTLU B,BAYRAKCEKEN K,yildirim S "Efficacy of Topical Boric Acid in the Treatment of Experimental Pseudomonas Aeruginosa Keratitis in Rats." Van Tıp Dergisi, 30, ss.60 - 65, 2023. 10.5505/vtd.2023.15425 |
| ISNAD | ondas, osman vd. "Efficacy of Topical Boric Acid in the Treatment of Experimental Pseudomonas Aeruginosa Keratitis in Rats". Van Tıp Dergisi 30/1 (2023), 60-65. https://doi.org/10.5505/vtd.2023.15425 |
