TY  - JOUR
TI  - The Effect of Whey Proteins on the Brain and Small Intestine Nitric Oxide Levels: Protein Profiles in Methotrexate-Induced Oxidative Stress
AB  - Objectives: The aim of this study was to determine the effects of whey proteins on methotrexate (MTX)-induced brain and small intestine damage. Materials and Methods: 30 Sprague Dawley rats (200-300 g) were divided into four groups: Control, control + whey, MTX, and MTX+whey. MTX was administered at 20 mg/kg (single dose) intraperitoneally to the MTX group rats, and 2 mg/kg of whey protein were administered by oral gavage for 10 days to the whey groups. Lipid peroxidation, glutathione, and nitric oxide (NO) levels, as well as glutathione-S- transferase and superoxide dismutase activities were measured in the brain and small intestine. SDS-polyacrylamide gel electrophoresis of the brain and intestine tissues were also carried out. Results: While MTX treatment caused oxidative damage in the brain and small intestine, whey protein administration ameliorated MTX- induced oxidative stress. MTX administration did not change the brain’s NO level, while an increase in intestinal NO level was detected. Conclusion: MTX induced oxidative stress in the brain and small intestine changed the protein metabolism in these tissues regardless of reduced food intake. Consecutive 10-day administration of whey proteins has shown its therapeutic effect on MTX-induced brain and small intestine oxidative damage.
AU  - Şener, Göksel
AU  - Gürel Gökmen, Begüm
AU  - DURSUN, Ercan
AU  - Tunali-Akbay, Tugba
AU  - Yılmaz Karaoğlu, Sümeyye
AU  - sivas, guzin goksun
AU  - Tufan, Elif
AU  - Özbeyli, Dilek
DO  - 10.26650/experimed.1189898
PY  - 2022
JO  - EXPERIMED
VL  - 12
IS  - 3
SN  - 2630-6050
SP  - 113
EP  - 118
DB  - TRDizin
UR  - http://search/yayin/detay/1166380
ER  -