Evaluation of the Effect of Circulating IncRNAs in Colorectal Cancers: As a Potential Biomarker

Yıl: 2022 Cilt: 37 Sayı: 3 Sayfa Aralığı: 305 - 313 Metin Dili: İngilizce DOI: 10.5505/tjo.2022.3575 İndeks Tarihi: 05-05-2023

Evaluation of the Effect of Circulating IncRNAs in Colorectal Cancers: As a Potential Biomarker

Öz:
This study aimed to investigate the possibilities of using the ARHGAP5-AS1, LOC152578, SNHG16, ZNRF3-IT1, CCAT1, CRNDE, and XLOC_000303 circulating lncRNAs as a non-invasive biomarker in colorectal cancers (CRCs). METHODS In this study, we enrolled plasma samples of 65 CRC patients (50 stages III/IV and 15 stages I/II) and plasma samples of 31 individuals in the control group of similar ages. Thereafter, we performed plasma separation and total RNA extraction; then, RNAs were reversely transcribed to complementary DNA. And then, we analyzed using a quantitative real time polymerase chain reaction technique for lncRNA expression analysis. RESULTS Our results showed that the expression levels of ZNRF3IT1 (p=0.011), CCAT1 (p=0.007), CRNDE (p=0.002), and XLOC_000303 (p=0.001) were significantly upregulated in the CRC when it was compared to with the control group. ZNRF3IT1, CCAT1, CRNDE, and XLOC_000303 lncRNAs were observed to have similar discriminating power. The calculated area under the curve of receiver operating characteristic was 0.66, 0.67, 0.70, and 0.70, respectively. CONCLUSION Our results revealed a high discriminatory power of ZNRF3-IT1, CCAT1, CRNDE, and XLOC_000303 lncRNA in distinguishing CRC patients from healthy individuals. We also found that increased XLOC_000303 expression is a protective factor against metastasis formation. This study demonstrated that ZNRF3-IT1, CCAT1, CRNDE, and XLOC_000303 circulating lncRNAs may be used as a potential non-invasive biomarker in CRC. We think that the increased expression of XLOC_000303 may be a protective factor against metastasis formation.
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APA Arslan S, Dincer M, Bayir Garbioglu D, EKER SARIBOYACI A, Erzurumluoglu Gokalp E, Kocagil S, Arık D, Ozen H, durak aras b, Artan S, ÇILINGIR O (2022). Evaluation of the Effect of Circulating IncRNAs in Colorectal Cancers: As a Potential Biomarker. , 305 - 313. 10.5505/tjo.2022.3575
Chicago Arslan SERAP,Dincer Murat,Bayir Garbioglu Duygu,EKER SARIBOYACI AYLA,Erzurumluoglu Gokalp Ebru,Kocagil Sinem,Arık Deniz,Ozen Hulya,durak aras beyhan,Artan Sevilhan,ÇILINGIR OGUZ Evaluation of the Effect of Circulating IncRNAs in Colorectal Cancers: As a Potential Biomarker. (2022): 305 - 313. 10.5505/tjo.2022.3575
MLA Arslan SERAP,Dincer Murat,Bayir Garbioglu Duygu,EKER SARIBOYACI AYLA,Erzurumluoglu Gokalp Ebru,Kocagil Sinem,Arık Deniz,Ozen Hulya,durak aras beyhan,Artan Sevilhan,ÇILINGIR OGUZ Evaluation of the Effect of Circulating IncRNAs in Colorectal Cancers: As a Potential Biomarker. , 2022, ss.305 - 313. 10.5505/tjo.2022.3575
AMA Arslan S,Dincer M,Bayir Garbioglu D,EKER SARIBOYACI A,Erzurumluoglu Gokalp E,Kocagil S,Arık D,Ozen H,durak aras b,Artan S,ÇILINGIR O Evaluation of the Effect of Circulating IncRNAs in Colorectal Cancers: As a Potential Biomarker. . 2022; 305 - 313. 10.5505/tjo.2022.3575
Vancouver Arslan S,Dincer M,Bayir Garbioglu D,EKER SARIBOYACI A,Erzurumluoglu Gokalp E,Kocagil S,Arık D,Ozen H,durak aras b,Artan S,ÇILINGIR O Evaluation of the Effect of Circulating IncRNAs in Colorectal Cancers: As a Potential Biomarker. . 2022; 305 - 313. 10.5505/tjo.2022.3575
IEEE Arslan S,Dincer M,Bayir Garbioglu D,EKER SARIBOYACI A,Erzurumluoglu Gokalp E,Kocagil S,Arık D,Ozen H,durak aras b,Artan S,ÇILINGIR O "Evaluation of the Effect of Circulating IncRNAs in Colorectal Cancers: As a Potential Biomarker." , ss.305 - 313, 2022. 10.5505/tjo.2022.3575
ISNAD Arslan, SERAP vd. "Evaluation of the Effect of Circulating IncRNAs in Colorectal Cancers: As a Potential Biomarker". (2022), 305-313. https://doi.org/10.5505/tjo.2022.3575
APA Arslan S, Dincer M, Bayir Garbioglu D, EKER SARIBOYACI A, Erzurumluoglu Gokalp E, Kocagil S, Arık D, Ozen H, durak aras b, Artan S, ÇILINGIR O (2022). Evaluation of the Effect of Circulating IncRNAs in Colorectal Cancers: As a Potential Biomarker. Türk Onkoloji Dergisi, 37(3), 305 - 313. 10.5505/tjo.2022.3575
Chicago Arslan SERAP,Dincer Murat,Bayir Garbioglu Duygu,EKER SARIBOYACI AYLA,Erzurumluoglu Gokalp Ebru,Kocagil Sinem,Arık Deniz,Ozen Hulya,durak aras beyhan,Artan Sevilhan,ÇILINGIR OGUZ Evaluation of the Effect of Circulating IncRNAs in Colorectal Cancers: As a Potential Biomarker. Türk Onkoloji Dergisi 37, no.3 (2022): 305 - 313. 10.5505/tjo.2022.3575
MLA Arslan SERAP,Dincer Murat,Bayir Garbioglu Duygu,EKER SARIBOYACI AYLA,Erzurumluoglu Gokalp Ebru,Kocagil Sinem,Arık Deniz,Ozen Hulya,durak aras beyhan,Artan Sevilhan,ÇILINGIR OGUZ Evaluation of the Effect of Circulating IncRNAs in Colorectal Cancers: As a Potential Biomarker. Türk Onkoloji Dergisi, vol.37, no.3, 2022, ss.305 - 313. 10.5505/tjo.2022.3575
AMA Arslan S,Dincer M,Bayir Garbioglu D,EKER SARIBOYACI A,Erzurumluoglu Gokalp E,Kocagil S,Arık D,Ozen H,durak aras b,Artan S,ÇILINGIR O Evaluation of the Effect of Circulating IncRNAs in Colorectal Cancers: As a Potential Biomarker. Türk Onkoloji Dergisi. 2022; 37(3): 305 - 313. 10.5505/tjo.2022.3575
Vancouver Arslan S,Dincer M,Bayir Garbioglu D,EKER SARIBOYACI A,Erzurumluoglu Gokalp E,Kocagil S,Arık D,Ozen H,durak aras b,Artan S,ÇILINGIR O Evaluation of the Effect of Circulating IncRNAs in Colorectal Cancers: As a Potential Biomarker. Türk Onkoloji Dergisi. 2022; 37(3): 305 - 313. 10.5505/tjo.2022.3575
IEEE Arslan S,Dincer M,Bayir Garbioglu D,EKER SARIBOYACI A,Erzurumluoglu Gokalp E,Kocagil S,Arık D,Ozen H,durak aras b,Artan S,ÇILINGIR O "Evaluation of the Effect of Circulating IncRNAs in Colorectal Cancers: As a Potential Biomarker." Türk Onkoloji Dergisi, 37, ss.305 - 313, 2022. 10.5505/tjo.2022.3575
ISNAD Arslan, SERAP vd. "Evaluation of the Effect of Circulating IncRNAs in Colorectal Cancers: As a Potential Biomarker". Türk Onkoloji Dergisi 37/3 (2022), 305-313. https://doi.org/10.5505/tjo.2022.3575