Evaluation of the Effect of Circulating IncRNAs in Colorectal Cancers: As a Potential Biomarker

Arslan, SERAP; durak aras, beyhan; Artan, Sevilhan; ÇILINGIR, OGUZ; Erzurumluoglu Gokalp, Ebru; Kocagil, Sinem; EKER SARIBOYACI, AYLA; Dincer, Murat; Bayir Garbioglu, Duygu; Arık, Deniz; Ozen, Hulya

doi:10.5505/tjo.2022.3575

Evaluation of the Effect of Circulating IncRNAs in Colorectal Cancers: As a Potential Biomarker

Serap ARSLAN, (Eskişehir Osmangazi Üniversitesi, Tıp Fakültesi, Tıbbi Genetik Anabilim Dalı, Eskişehir, Türkiye)

Murat DİNÇER, (Eskişehir Osmangazi Üniversitesi, Tıp Fakültesi, Tıbbi Onkoloji Anabilim Dalı, Eskişehir, Türkiye)

Duygu BAYIR, (Eskişehir Osmangazi Üniversitesi, Tıp Fakültesi, Tıbbi Onkoloji Anabilim Dalı, Eskişehir, Türkiye)

Ayla SARIBOYACI, (Eskişehir Osmangazi Üniversitesi, Hücresel Tedavi ve Kök Hücre Üretimi Uygulama ve Araştırma Merkezi (ESTEM), Kök Hücre Anabilim Dalı Eskişehir, Türkiye)

Ebru ERZURUMLUOĞLU GÖKALP, (Eskişehir Osmangazi Üniversitesi, Tıp Fakültesi, Tıbbi Genetik Anabilim Dalı, Eskişehir, Türkiye)

Sinem KOCAGİL, (Eskişehir Osmangazi Üniversitesi, Tıp Fakültesi, Tıbbi Genetik Anabilim Dalı, Eskişehir, Türkiye)

Deniz ARIK, (Eskişehir Osmangazi Üniversitesi, Tıp Fakültesi, Patoloji Anabilim Dalı, Eskişehir, Türkiye)

Hülya ÖZEN, (Sağlık Bilimleri Üniversitesi, Gülhane Tıp Fakültesi, Tıp Bilişimi Anabilim Dalı, Ankara, Türkiye)

Beyhan DURAK ARAS, (Eskişehir Osmangazi Üniversitesi, Tıp Fakültesi, Tıbbi Genetik Anabilim Dalı, Eskişehir, Türkiye)

Sevilhan ARTAN, (Eskişehir Osmangazi Üniversitesi, Tıp Fakültesi, Tıbbi Genetik Anabilim Dalı, Eskişehir, Türkiye)

Oğuz ÇİLİNGİR, (Eskişehir Osmangazi Üniversitesi, Tıp Fakültesi, Tıbbi Genetik Anabilim Dalı, Eskişehir, Türkiye)

Diğer Yazarlar

Türk Onkoloji Dergisi

2 2

Yıl: 2022 Cilt: 37 Sayı: 3 Sayfa Aralığı: 305 - 313 Metin Dili: İngilizce DOI: 10.5505/tjo.2022.3575 İndeks Tarihi: 05-05-2023

Evaluation of the Effect of Circulating IncRNAs in Colorectal Cancers: As a Potential Biomarker

Öz:

This study aimed to investigate the possibilities of using the ARHGAP5-AS1, LOC152578, SNHG16, ZNRF3-IT1, CCAT1, CRNDE, and XLOC_000303 circulating lncRNAs as a non-invasive biomarker in colorectal cancers (CRCs). METHODS In this study, we enrolled plasma samples of 65 CRC patients (50 stages III/IV and 15 stages I/II) and plasma samples of 31 individuals in the control group of similar ages. Thereafter, we performed plasma separation and total RNA extraction; then, RNAs were reversely transcribed to complementary DNA. And then, we analyzed using a quantitative real time polymerase chain reaction technique for lncRNA expression analysis. RESULTS Our results showed that the expression levels of ZNRF3IT1 (p=0.011), CCAT1 (p=0.007), CRNDE (p=0.002), and XLOC_000303 (p=0.001) were significantly upregulated in the CRC when it was compared to with the control group. ZNRF3IT1, CCAT1, CRNDE, and XLOC_000303 lncRNAs were observed to have similar discriminating power. The calculated area under the curve of receiver operating characteristic was 0.66, 0.67, 0.70, and 0.70, respectively. CONCLUSION Our results revealed a high discriminatory power of ZNRF3-IT1, CCAT1, CRNDE, and XLOC_000303 lncRNA in distinguishing CRC patients from healthy individuals. We also found that increased XLOC_000303 expression is a protective factor against metastasis formation. This study demonstrated that ZNRF3-IT1, CCAT1, CRNDE, and XLOC_000303 circulating lncRNAs may be used as a potential non-invasive biomarker in CRC. We think that the increased expression of XLOC_000303 may be a protective factor against metastasis formation.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Arslan S, Dincer M, Bayir Garbioglu D, EKER SARIBOYACI A, Erzurumluoglu Gokalp E, Kocagil S, Arık D, Ozen H, durak aras b, Artan S, ÇILINGIR O (2022). Evaluation of the Effect of Circulating IncRNAs in Colorectal Cancers: As a Potential Biomarker. , 305 - 313. 10.5505/tjo.2022.3575
Chicago	Arslan SERAP,Dincer Murat,Bayir Garbioglu Duygu,EKER SARIBOYACI AYLA,Erzurumluoglu Gokalp Ebru,Kocagil Sinem,Arık Deniz,Ozen Hulya,durak aras beyhan,Artan Sevilhan,ÇILINGIR OGUZ Evaluation of the Effect of Circulating IncRNAs in Colorectal Cancers: As a Potential Biomarker. (2022): 305 - 313. 10.5505/tjo.2022.3575
MLA	Arslan SERAP,Dincer Murat,Bayir Garbioglu Duygu,EKER SARIBOYACI AYLA,Erzurumluoglu Gokalp Ebru,Kocagil Sinem,Arık Deniz,Ozen Hulya,durak aras beyhan,Artan Sevilhan,ÇILINGIR OGUZ Evaluation of the Effect of Circulating IncRNAs in Colorectal Cancers: As a Potential Biomarker. , 2022, ss.305 - 313. 10.5505/tjo.2022.3575
AMA	Arslan S,Dincer M,Bayir Garbioglu D,EKER SARIBOYACI A,Erzurumluoglu Gokalp E,Kocagil S,Arık D,Ozen H,durak aras b,Artan S,ÇILINGIR O Evaluation of the Effect of Circulating IncRNAs in Colorectal Cancers: As a Potential Biomarker. . 2022; 305 - 313. 10.5505/tjo.2022.3575
Vancouver	Arslan S,Dincer M,Bayir Garbioglu D,EKER SARIBOYACI A,Erzurumluoglu Gokalp E,Kocagil S,Arık D,Ozen H,durak aras b,Artan S,ÇILINGIR O Evaluation of the Effect of Circulating IncRNAs in Colorectal Cancers: As a Potential Biomarker. . 2022; 305 - 313. 10.5505/tjo.2022.3575
IEEE	Arslan S,Dincer M,Bayir Garbioglu D,EKER SARIBOYACI A,Erzurumluoglu Gokalp E,Kocagil S,Arık D,Ozen H,durak aras b,Artan S,ÇILINGIR O "Evaluation of the Effect of Circulating IncRNAs in Colorectal Cancers: As a Potential Biomarker." , ss.305 - 313, 2022. 10.5505/tjo.2022.3575
ISNAD	Arslan, SERAP vd. "Evaluation of the Effect of Circulating IncRNAs in Colorectal Cancers: As a Potential Biomarker". (2022), 305-313. https://doi.org/10.5505/tjo.2022.3575

APA	Arslan S, Dincer M, Bayir Garbioglu D, EKER SARIBOYACI A, Erzurumluoglu Gokalp E, Kocagil S, Arık D, Ozen H, durak aras b, Artan S, ÇILINGIR O (2022). Evaluation of the Effect of Circulating IncRNAs in Colorectal Cancers: As a Potential Biomarker. Türk Onkoloji Dergisi, 37(3), 305 - 313. 10.5505/tjo.2022.3575
Chicago	Arslan SERAP,Dincer Murat,Bayir Garbioglu Duygu,EKER SARIBOYACI AYLA,Erzurumluoglu Gokalp Ebru,Kocagil Sinem,Arık Deniz,Ozen Hulya,durak aras beyhan,Artan Sevilhan,ÇILINGIR OGUZ Evaluation of the Effect of Circulating IncRNAs in Colorectal Cancers: As a Potential Biomarker. Türk Onkoloji Dergisi 37, no.3 (2022): 305 - 313. 10.5505/tjo.2022.3575
MLA	Arslan SERAP,Dincer Murat,Bayir Garbioglu Duygu,EKER SARIBOYACI AYLA,Erzurumluoglu Gokalp Ebru,Kocagil Sinem,Arık Deniz,Ozen Hulya,durak aras beyhan,Artan Sevilhan,ÇILINGIR OGUZ Evaluation of the Effect of Circulating IncRNAs in Colorectal Cancers: As a Potential Biomarker. Türk Onkoloji Dergisi, vol.37, no.3, 2022, ss.305 - 313. 10.5505/tjo.2022.3575
AMA	Arslan S,Dincer M,Bayir Garbioglu D,EKER SARIBOYACI A,Erzurumluoglu Gokalp E,Kocagil S,Arık D,Ozen H,durak aras b,Artan S,ÇILINGIR O Evaluation of the Effect of Circulating IncRNAs in Colorectal Cancers: As a Potential Biomarker. Türk Onkoloji Dergisi. 2022; 37(3): 305 - 313. 10.5505/tjo.2022.3575
Vancouver	Arslan S,Dincer M,Bayir Garbioglu D,EKER SARIBOYACI A,Erzurumluoglu Gokalp E,Kocagil S,Arık D,Ozen H,durak aras b,Artan S,ÇILINGIR O Evaluation of the Effect of Circulating IncRNAs in Colorectal Cancers: As a Potential Biomarker. Türk Onkoloji Dergisi. 2022; 37(3): 305 - 313. 10.5505/tjo.2022.3575
IEEE	Arslan S,Dincer M,Bayir Garbioglu D,EKER SARIBOYACI A,Erzurumluoglu Gokalp E,Kocagil S,Arık D,Ozen H,durak aras b,Artan S,ÇILINGIR O "Evaluation of the Effect of Circulating IncRNAs in Colorectal Cancers: As a Potential Biomarker." Türk Onkoloji Dergisi, 37, ss.305 - 313, 2022. 10.5505/tjo.2022.3575
ISNAD	Arslan, SERAP vd. "Evaluation of the Effect of Circulating IncRNAs in Colorectal Cancers: As a Potential Biomarker". Türk Onkoloji Dergisi 37/3 (2022), 305-313. https://doi.org/10.5505/tjo.2022.3575