Evaluation of Bax protein in breast cancer cells treated with tannic acid

Turgut Cosan, Didem; Değirmenci, İrfan; GÜNEŞ, HASAN VEYSİ; SOYOCAK, AHU; BAŞARAN, AYŞE YELDA

Evaluation of Bax protein in breast cancer cells treated with tannic acid

Ahu SOYOCAK, (Osmangazi Üniversitesi, Tıp Fakültesi, Tıbbi Biyoloji Anabilim Dalı, Eskişehir, Türkiye)

Didem Turgut COŞAN, (Osmangazi Üniversitesi, Tıp Fakültesi, Tıbbi Biyoloji Anabilim Dalı, Eskişehir, Türkiye)

AYŞE YELDA BAŞARAN, (Osmangazi Üniversitesi, Tıp Fakültesi, Tıbbi Biyoloji Anabilim Dalı, Eskişehir, Türkiye)

Hasan Veysi GÜNEŞ, (Osmangazi Üniversitesi, Tıp Fakültesi, Tıbbi Biyoloji Anabilim Dalı, Eskişehir, Türkiye)

İrfan DEĞİRMENCİ (Osmangazi Üniversitesi, Tıp Fakültesi, Tıbbi Biyoloji Anabilim Dalı, Eskişehir, Türkiye)

Dicle Tıp Dergisi

6 0

Yıl: 2011 Cilt: 38 Sayı: 1 Sayfa Aralığı: 1 - 6 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Evaluation of Bax protein in breast cancer cells treated with tannic acid

Öz:

Amaç: Bir bitki polifenolü olan Tannik Asit (TA)’in antikarsinojenik, antioksidan, antimutajenik, antimikrobiyal, antiallerjik ve antiinflamatuar aktiviteleri vardır. Bununla birlikte şimdiye kadar, meme kanserinde TA’in antikanser aktivitesinden sorumlu kesin bir mekanizma henüz açıkça tanımlanamamıştır. Bu çalışmanın amacı, insan meme kanser hücre dizisinde (MCF-7) TA’in pro-apoptotik Bax proteini üzerine etkisini araştırmaktır. Gereç ve yöntem: Bu çalışmada meme kanser hücrelerine (MCF-7) çeşitli konsantrasyon (0, 25, 50 and 100 μM) ve saatlerde (24, 48 ve 72. saatlerde) TA uygulandı. Bu hücrelerdeki pro-apoptotik Bax protein yüzdesini belirlemek için immunohistokimyasal boyama yöntemi kullanıldı. Bulgular: Araştırmamızın sonunda, çeşitli dozlarda TA muamelesinden sonra kanser hücrelerindeki pro-apoptotik Bax proteini yüzdesinin zamana bağımlı olarak arttığı gözlemledik. Bu artışın özellikle 72. saat’te ve 25 μM’lük dozda en yüksek düzeyde olduğu görüldü. Sonuç: Bulgularımıza göre, TA’in Bax proteinini artırarak, meme kanseri hücrelerinin apoptoza gitmesini sağlayabileceğini düşünmekteyiz. Bu çalışmadaki sonucun desteklenebilmesi için TA’in apoptotik yoldaki diğer proteinlerle ilişkisinin ayrıntılı bir şekilde değerlendirilmesi gerekmektedir.

Anahtar Kelime:

Konular: Cerrahi

Tannik asit uygulanan meme kanseri hücrelerinde Bax proteinin değerlendirilmesi

Öz:

Objectives: Tannic acid (TA), a plant polyphenol, is known to have anti-carcinogenic, anti-oxidant, anti-mutagenic, anti-microbial, anti-allergic, anti-inflammatory activities. However, a precise mechanism responsible for the anti-cancer activity of TA in breast cancer has not yet been clearly described. The aim of this study was to investigate the effect of TA on the pro-apoptotic Bax protein in a human breast cancer cell line (MCF-7). Materials and methods: In this study, TA in various concentrations (0, 25, 50 and 100 μM) were administrated at various time points (24th h, 48th h and 72nd h) to breast cancer cells (MCF-7). The percentages of pro-apoptotic Bax protein in these cells were determined by immunohistochemical staining. Results: At the completion of the study, percentages of the pro-apoptotic Bax protein in pro-apoptotic cancer cells were found to be increased in a time-dependent manner after exposure to various concentrations of TA. This increase was at highest level with the concentration of 25 μM at 72nd hour. Conclusion: Based on our results, we suggested that TA could induce apoptosis of breast cancer cells by increasing Bax protein. Further studies evaluating the relationship of TA with other proteins that have a role in apoptotic pathways are warranted to support the findings of this study.

Anahtar Kelime:

Konular: Cerrahi

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	SOYOCAK A, Turgut Cosan D, BAŞARAN A, GÜNEŞ H, Değirmenci İ (2011). Evaluation of Bax protein in breast cancer cells treated with tannic acid. , 1 - 6.
Chicago	SOYOCAK AHU,Turgut Cosan Didem,BAŞARAN AYŞE YELDA,GÜNEŞ HASAN VEYSİ,Değirmenci İrfan Evaluation of Bax protein in breast cancer cells treated with tannic acid. (2011): 1 - 6.
MLA	SOYOCAK AHU,Turgut Cosan Didem,BAŞARAN AYŞE YELDA,GÜNEŞ HASAN VEYSİ,Değirmenci İrfan Evaluation of Bax protein in breast cancer cells treated with tannic acid. , 2011, ss.1 - 6.
AMA	SOYOCAK A,Turgut Cosan D,BAŞARAN A,GÜNEŞ H,Değirmenci İ Evaluation of Bax protein in breast cancer cells treated with tannic acid. . 2011; 1 - 6.
Vancouver	SOYOCAK A,Turgut Cosan D,BAŞARAN A,GÜNEŞ H,Değirmenci İ Evaluation of Bax protein in breast cancer cells treated with tannic acid. . 2011; 1 - 6.
IEEE	SOYOCAK A,Turgut Cosan D,BAŞARAN A,GÜNEŞ H,Değirmenci İ "Evaluation of Bax protein in breast cancer cells treated with tannic acid." , ss.1 - 6, 2011.
ISNAD	SOYOCAK, AHU vd. "Evaluation of Bax protein in breast cancer cells treated with tannic acid". (2011), 1-6.

APA	SOYOCAK A, Turgut Cosan D, BAŞARAN A, GÜNEŞ H, Değirmenci İ (2011). Evaluation of Bax protein in breast cancer cells treated with tannic acid. Dicle Tıp Dergisi, 38(1), 1 - 6.
Chicago	SOYOCAK AHU,Turgut Cosan Didem,BAŞARAN AYŞE YELDA,GÜNEŞ HASAN VEYSİ,Değirmenci İrfan Evaluation of Bax protein in breast cancer cells treated with tannic acid. Dicle Tıp Dergisi 38, no.1 (2011): 1 - 6.
MLA	SOYOCAK AHU,Turgut Cosan Didem,BAŞARAN AYŞE YELDA,GÜNEŞ HASAN VEYSİ,Değirmenci İrfan Evaluation of Bax protein in breast cancer cells treated with tannic acid. Dicle Tıp Dergisi, vol.38, no.1, 2011, ss.1 - 6.
AMA	SOYOCAK A,Turgut Cosan D,BAŞARAN A,GÜNEŞ H,Değirmenci İ Evaluation of Bax protein in breast cancer cells treated with tannic acid. Dicle Tıp Dergisi. 2011; 38(1): 1 - 6.
Vancouver	SOYOCAK A,Turgut Cosan D,BAŞARAN A,GÜNEŞ H,Değirmenci İ Evaluation of Bax protein in breast cancer cells treated with tannic acid. Dicle Tıp Dergisi. 2011; 38(1): 1 - 6.
IEEE	SOYOCAK A,Turgut Cosan D,BAŞARAN A,GÜNEŞ H,Değirmenci İ "Evaluation of Bax protein in breast cancer cells treated with tannic acid." Dicle Tıp Dergisi, 38, ss.1 - 6, 2011.
ISNAD	SOYOCAK, AHU vd. "Evaluation of Bax protein in breast cancer cells treated with tannic acid". Dicle Tıp Dergisi 38/1 (2011), 1-6.