Objective: This study was conducted to determine the levels of pain, physical activity and kinesiophobia of individuals with knee osteoarthritis and to examine the relationship between these variables. Material and Methods: This cross-sectional and analytical study was conducted on 290 people who applied to the physical therapy and rehabilitation center of a university hospital in the Central Black Sea Region, diagnosed with bilateral knee osteoarthritis at least 6 months ago, and had a pain level of 4 or more on the Visual Analogue Scale- Pain. Results: Indicating that women, individuals who are single, people with chronic diseases and using drugs when they have pain; pain, physical function, stiffness and kinesiophobia mean scores were higher than other groups (p<0.05). Most of the individuals had low physical activity levels. There is a moderately positive correlation between kinesiophobia score and pain, physical function, stiffness scores (p<0.05). At the same time, there was a significant positive correlation between pain score and stiffness, physical function scores (p<0.05). Conclusions: It was determined that the total mean score of pain and kinesiophobia of individuals with osteoarthritis was moderate, physical activity score is low. Female gender, being single, having a chronic disease and using medication when in pain that has been determined that there are important factors affecting pain and kinesiophobia. It is important for nurses to evaluate pain and decrease in physical activity and kinesiophobia levels in individuals with osteoarthritis at an early stage.