Antidiabetic and Antioxidant Effect of the Aerial Parts of Lysimachia verticillaris and its Isolated Phenolic Compounds on Streptozotocin-induced Diabetic Rats
Yıl: 2022 Cilt: 10 Sayı: 4 Sayfa Aralığı: 434 - 441 Metin Dili: İngilizce DOI: 10.14235/bas.galenos.2021.6123 İndeks Tarihi: 08-05-2023
Antidiabetic and Antioxidant Effect of the Aerial Parts of Lysimachia verticillaris and its Isolated Phenolic Compounds on Streptozotocin-induced Diabetic Rats
Öz: Objective: Lysimachia genus has been recorded to be used in diabetes, traditionally and to have antidiabetic effect and antioxidant effects. The aim of this study was to evaluate the antidiabetic and antioxidant effects of aqueous extract of Lysimachia verticillaris (LV) and its isolated compounds, the percentage of apoptosis and histological changes in pancreatic β-cells in rats with streptozotocin (STZ)-induced type 1 diabetes mellitus. Methods: Male Spraque Dawley rats were divided into 6 groups. STZ (40 mg/kg) induced diabetic rats were treated orally with aqueous extract (400 mg/kg) and isolated compounds (20 mg/ kg). To interpret antidiabetic effect, serum glucose and insulin levels were measured and morphological changes of pancreas were examined. Serum samples were analysed for catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPX) levels to investigate antioxidant effect. The terminal deoxynucleotidyl transferase (TDT)-mediated dUTP-biotin nick end-labeling (TUNEL) assays were performed to reveal apoptosis of pancreatic β-cells. Results: Oral administration of the extract and isolated compounds reduced high blood glucose levels (p<0.005). Morever, the treatments were resulted in increased serum insulin (p≤0.05), CAT (p≤0.05), SOD (p≤0.05) and GPX (p≤0.05). All applications with compounds, especially with quercetin 3-O- β-glucopyranoside reduced the morphological impairment of pancreas. The percentage of TUNEL positive cells was higher (p≤0.05) in the pancreatic islets of untreated diabetic group than others groups. Conclusion: According to results, the extract and isolated compounds of LV displayed antidiabetic effect, while quercetin 3-O-β-glucopyranoside showed the highest antidiabetic effect. The antidiabetic effects of LV and phenolic compounds may be due to their antioxidant effects. Thus, LV and isolated compounds can be a potential source of herbal medicine for DM. However, it requires further investigations such as toxicological analysis studies and clinical trials.
Anahtar Kelime: Lysimachia verticillaris’in Toprak Üstü Kısmının ve İzole Edilmiş Bileşiklerinin StreptozotosinileIndüklenenDiyabetikSıçanlardaAntidiyabetikveAntioksidan Etkisi
Öz: Amaç: Lysimachia cinsinin geleneksel olarak diyabet için kullanıldığı ve antidiyabetik ve antioksidan etkilere sahip olduğu kaydedilmiştir. Bu çalışmanın amacı, Lysimachia verticillaris’in (LV) su ekstresinin ve izole edilmiş bileşiklerinin streptozotosin (STZ) ile tip I diyabet oluşturulan sıçanlardaki antidiyabetik ve antioksidan etkilerini, pankreas β-hücrelerindeki apoptoz yüzdesini ve histolojik değişiklikleri değerlendirmektir. Yöntemler: Erkek Spraque Dawley cinsi sıçanlar 6 gruba ayrılmıştır. STZ (40 mg/kg) ile indüklenen diyabetik sıçanlara, su ekstresi (400 mg/kg) ve izole bileşikler (20 mg/kg) oral yoldan uygulanmıştır. Deney sonunda antidiyabetik etkiyi yorumlamak için kan glukozu ve serum insülin düzeyleri tespit edilmiştir. Pankreastaki morfolojik değişiklikler incelenmiştir. Serum biyokimyasal parametreleri, katalaz (CAT), süperoksit dismutaz (SOD), glutatyon peroksidaz (GPX) açısından da antioksidan etkiyi araştırmak için analiz edilmiştir. Ayrıca pankreas β-hücrelerinin apoptozunun tespiti için terminal deoksinükleotidil transferaz aracılı dUTP-biyotin çentik uç etiketleme (TUNEL) deneyleri gerçekleştirilmiştir. Bulgular: Ekstre ve izole bileşiklerin oral uygulamasının yüksek kan glikoz seviyelerini düşürdüğü saptanmıştır (p<0,005). Oral uygulamalar, serum insülin (p≤0,05), CAT (p≤0,05), SOD (p≤0,05) ve GPX (p≤0,05) düzeylerinin artışıyla sonuçlanmıştır. Ayrıca, kersetin 3-O-β-glikopiranozit için belirgin olmakla birlikte, uygulamaların pankreasın morfolojik bozukluğunu azalttığı saptanmıştır. TUNEL pozitif hücrelerin yüzdesi, tedavi edilmeyen diyabetik grubun pankreas adacıklarında diğer gruplara göre daha yüksek gözlenmiştir (p≤0,05). Sonuç: Sonuçlara göre, LV’nin ekstresi ve izole edilmiş bileşikleri antidiyabetik etki gösterirken, kersetin 3-O- β-glikopiranozit en yüksek antidiyabetik etkiyi sergilemiştir. LV ve fenolik bileşiklerin antidiyabetik etkileri, DM’den kaynaklanan hasarlar üzerindeki antioksidan etkilerinden kaynaklanıyor olabilir. Bu nedenle, LV ve izole bileşikleri, diyabet ve komplikasyonları için potansiyel bir bitkisel ilaç kaynağı olabilir. Bununla birlikte, yeni bitkisel ilaçlar oluşturmak, toksikolojik analiz çalışmaları ve klinik araştırmalar gibi daha ileri araştırmalar gerektirir.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | SENER S, badem m, ÖZGEN U, Korkmaz N, KANBOLAT Ş, KERİMOĞLU G, aliyazicioglu r, Yenilmez E, Sezen Karaoğlan E (2022). Antidiabetic and Antioxidant Effect of the Aerial Parts of Lysimachia verticillaris and its Isolated Phenolic Compounds on Streptozotocin-induced Diabetic Rats. , 434 - 441. 10.14235/bas.galenos.2021.6123 |
| Chicago | SENER SILA OZLEM,badem merve,ÖZGEN Ufuk,Korkmaz Nuriye,KANBOLAT Şeyda,KERİMOĞLU Gökçen,aliyazicioglu rezzan,Yenilmez Engin,Sezen Karaoğlan Esen Antidiabetic and Antioxidant Effect of the Aerial Parts of Lysimachia verticillaris and its Isolated Phenolic Compounds on Streptozotocin-induced Diabetic Rats. (2022): 434 - 441. 10.14235/bas.galenos.2021.6123 |
| MLA | SENER SILA OZLEM,badem merve,ÖZGEN Ufuk,Korkmaz Nuriye,KANBOLAT Şeyda,KERİMOĞLU Gökçen,aliyazicioglu rezzan,Yenilmez Engin,Sezen Karaoğlan Esen Antidiabetic and Antioxidant Effect of the Aerial Parts of Lysimachia verticillaris and its Isolated Phenolic Compounds on Streptozotocin-induced Diabetic Rats. , 2022, ss.434 - 441. 10.14235/bas.galenos.2021.6123 |
| AMA | SENER S,badem m,ÖZGEN U,Korkmaz N,KANBOLAT Ş,KERİMOĞLU G,aliyazicioglu r,Yenilmez E,Sezen Karaoğlan E Antidiabetic and Antioxidant Effect of the Aerial Parts of Lysimachia verticillaris and its Isolated Phenolic Compounds on Streptozotocin-induced Diabetic Rats. . 2022; 434 - 441. 10.14235/bas.galenos.2021.6123 |
| Vancouver | SENER S,badem m,ÖZGEN U,Korkmaz N,KANBOLAT Ş,KERİMOĞLU G,aliyazicioglu r,Yenilmez E,Sezen Karaoğlan E Antidiabetic and Antioxidant Effect of the Aerial Parts of Lysimachia verticillaris and its Isolated Phenolic Compounds on Streptozotocin-induced Diabetic Rats. . 2022; 434 - 441. 10.14235/bas.galenos.2021.6123 |
| IEEE | SENER S,badem m,ÖZGEN U,Korkmaz N,KANBOLAT Ş,KERİMOĞLU G,aliyazicioglu r,Yenilmez E,Sezen Karaoğlan E "Antidiabetic and Antioxidant Effect of the Aerial Parts of Lysimachia verticillaris and its Isolated Phenolic Compounds on Streptozotocin-induced Diabetic Rats." , ss.434 - 441, 2022. 10.14235/bas.galenos.2021.6123 |
| ISNAD | SENER, SILA OZLEM vd. "Antidiabetic and Antioxidant Effect of the Aerial Parts of Lysimachia verticillaris and its Isolated Phenolic Compounds on Streptozotocin-induced Diabetic Rats". (2022), 434-441. https://doi.org/10.14235/bas.galenos.2021.6123 |
| APA | SENER S, badem m, ÖZGEN U, Korkmaz N, KANBOLAT Ş, KERİMOĞLU G, aliyazicioglu r, Yenilmez E, Sezen Karaoğlan E (2022). Antidiabetic and Antioxidant Effect of the Aerial Parts of Lysimachia verticillaris and its Isolated Phenolic Compounds on Streptozotocin-induced Diabetic Rats. Bezmiâlem Science, 10(4), 434 - 441. 10.14235/bas.galenos.2021.6123 |
| Chicago | SENER SILA OZLEM,badem merve,ÖZGEN Ufuk,Korkmaz Nuriye,KANBOLAT Şeyda,KERİMOĞLU Gökçen,aliyazicioglu rezzan,Yenilmez Engin,Sezen Karaoğlan Esen Antidiabetic and Antioxidant Effect of the Aerial Parts of Lysimachia verticillaris and its Isolated Phenolic Compounds on Streptozotocin-induced Diabetic Rats. Bezmiâlem Science 10, no.4 (2022): 434 - 441. 10.14235/bas.galenos.2021.6123 |
| MLA | SENER SILA OZLEM,badem merve,ÖZGEN Ufuk,Korkmaz Nuriye,KANBOLAT Şeyda,KERİMOĞLU Gökçen,aliyazicioglu rezzan,Yenilmez Engin,Sezen Karaoğlan Esen Antidiabetic and Antioxidant Effect of the Aerial Parts of Lysimachia verticillaris and its Isolated Phenolic Compounds on Streptozotocin-induced Diabetic Rats. Bezmiâlem Science, vol.10, no.4, 2022, ss.434 - 441. 10.14235/bas.galenos.2021.6123 |
| AMA | SENER S,badem m,ÖZGEN U,Korkmaz N,KANBOLAT Ş,KERİMOĞLU G,aliyazicioglu r,Yenilmez E,Sezen Karaoğlan E Antidiabetic and Antioxidant Effect of the Aerial Parts of Lysimachia verticillaris and its Isolated Phenolic Compounds on Streptozotocin-induced Diabetic Rats. Bezmiâlem Science. 2022; 10(4): 434 - 441. 10.14235/bas.galenos.2021.6123 |
| Vancouver | SENER S,badem m,ÖZGEN U,Korkmaz N,KANBOLAT Ş,KERİMOĞLU G,aliyazicioglu r,Yenilmez E,Sezen Karaoğlan E Antidiabetic and Antioxidant Effect of the Aerial Parts of Lysimachia verticillaris and its Isolated Phenolic Compounds on Streptozotocin-induced Diabetic Rats. Bezmiâlem Science. 2022; 10(4): 434 - 441. 10.14235/bas.galenos.2021.6123 |
| IEEE | SENER S,badem m,ÖZGEN U,Korkmaz N,KANBOLAT Ş,KERİMOĞLU G,aliyazicioglu r,Yenilmez E,Sezen Karaoğlan E "Antidiabetic and Antioxidant Effect of the Aerial Parts of Lysimachia verticillaris and its Isolated Phenolic Compounds on Streptozotocin-induced Diabetic Rats." Bezmiâlem Science, 10, ss.434 - 441, 2022. 10.14235/bas.galenos.2021.6123 |
| ISNAD | SENER, SILA OZLEM vd. "Antidiabetic and Antioxidant Effect of the Aerial Parts of Lysimachia verticillaris and its Isolated Phenolic Compounds on Streptozotocin-induced Diabetic Rats". Bezmiâlem Science 10/4 (2022), 434-441. https://doi.org/10.14235/bas.galenos.2021.6123 |
