Effects of Sulfur Containing Glycine Imine Derivatives Compounds on Multidrug Resistance Proteins (MRPs) and Apoptosis Mechanism in MCF-7 and DLD-1 Cell Lines

Gul, Melek; Yıldırım, Tuba; Mesci, Seda; YAZGAN, BURAK

doi:10.14235/bas.galenos.2021.6306

Effects of Sulfur Containing Glycine Imine Derivatives Compounds on Multidrug Resistance Proteins (MRPs) and Apoptosis Mechanism in MCF-7 and DLD-1 Cell Lines

Seda MESCİ, (Hitit Üniversitesi, Bilimsel Teknik Uygulama ve Araştırma Merkezi, Çorum, TÜRKİYE)

Burak YAZGAN, (Amasya Üniversitesi, Sabuncuoğlu Şerefeddin Sağlık Hizmetleri Meslek Yüksekokulu, Tıbbi Hizmetler ve Teknikler Anabilim Dalı, Amasya, TÜRKİYE)

Melek GÜL, (Amasya Üniversitesi, Fen Bilimleri Enstitüsü, Biyoteknoloji Anabilim Dalı, Amasya, TÜRKİYE)

Tuba YILDIRIM (Amasya Üniversitesi, Fen Bilimleri Enstitüsü, Biyoteknoloji Anabilim Dalı, Amasya, TÜRKİYE)

Bezmiâlem Science

3 2

Yıl: 2022 Cilt: 10 Sayı: 4 Sayfa Aralığı: 461 - 469 Metin Dili: İngilizce DOI: 10.14235/bas.galenos.2021.6306 İndeks Tarihi: 08-05-2023

Effects of Sulfur Containing Glycine Imine Derivatives Compounds on Multidrug Resistance Proteins (MRPs) and Apoptosis Mechanism in MCF-7 and DLD-1 Cell Lines

Öz:

Objective: Glutathione (GSH) is a tripeptide consisting of glycine, glutamic acid and cysteine. If the sulfur containing amino acids like methionine and cysteine increase in the cells, the level of GSH increases. GSH is one of the most important and powerful antioxidants in the body and it reduces oxidative stress. Reduction of GSH affects apoptosis activity and multidrug resistance proteins (MRPs). In the present study, we investigated the effects of sulfur- containing glycine imine derivatives on MRPs and apoptosis mechanism in the MCF-7 (breast cancer) and DLD-1 (colon cancer) cell lines. Methods: In MCF-7 and DLD-1 cell lines; mRNA levels of MRPs (ABCB1, ABCC3, ABCC10, ABCC11 and ABCG2), apoptosis mechanism proteins (BAX, BACL-2, P53, PARP, CASP3), heat shock proteins (HSPs) and endoplasmic reticulum chaperone proteins (GRPs) were determined by qRT-PCR method. Results: Compounds decreased gene expression of multiple drug resistance (MDR) genes and increased gene expression of pro- apoptosis mechanism genes (BAX, P53, CASP3). HSPs and BCL- 2 and PARP gene expressions decreased. There was no significant decrease in gene expression of GRPs. The compounds were shown to have remarkable effects on MDR genes (ABCB1, ABCC10, ABCC11 and ABCG2) other than ABCC3. Compounds were found to have significant effects on apoptosis mechanism genes and HSPs. Conclusion: Our results indicate that the sulfur-containing glycine imine derivatives can be a potent option as a cancer drug, especially in breast and colon cancers.

Anahtar Kelime:

Kükürt İçeren Gilisin İmin Türevi Bileşiklerin MCF-7 ve DLD-1 Hücre Hatlarında Çoklu İlaç Direnci Proteinleri (MRP’ler) ve Apoptoz Mekanizması Üzerine Etkileri

Öz:

Amaç: Glutatyon (GSH), glisin, glutamik asit ve sisteinden oluşan bir tripeptiddir. Metiyonin ve sistein gibi kükürt içeren amino asitler hücrelerde artarsa GSH seviyesi yükselmektedir. GSH vücuttaki en önemli ve güçlü antioksidanlardan biridir ve oksidatif stresi azaltmaktadır. GSH’nin azaltılması, apoptoz aktivitesini ve çoklu ilaç direnci proteinlerini (MRP’ler) etkilemektedir. Bu çalışmada, kükürt içeren glisin imin türevlerinin, MCF-7 (meme kanseri) ve DLD-1 (kolon kanseri) hücre hatlarında çoklu ilaç direnci proteinlerine (MRP’ler) ve apoptoz mekanizmasına etkisini araştırmayı amaçladık. Yöntemler: MCF-7 ve DLD-1 hücre hatlarında; çoklu ilaç direnci proteinleri (ABCB1, ABCC3, ABCC10, ABCC11 ve ABCG2), apoptoz mekanizması proteinleri (BAX, BACL-2, P53, PARP, CASP3), ısı şoku proteinleri (HSP’ler) ve endoplazmik retikulum (ER) GRP’ler mRNA seviyeleri qRT-PCR yöntemi ile belirlendi. Bulgular: Bileşikler çoklu ilaç direncinde (MDR) gen ekspresyonunu azalttı ve pro-apoptoz mekanizması genlerinde (BAX, P53, CASP3) ekspresyonu artırdı. HSP'ler, BCL-2 ve PARP gen ekspresyonları azaldı. GRP gen ekspresyonunda önemli bir azalma olmadı. Bileşiklerin, ABCC3 dışındaki çoklu ilaç direnci genleri (ABCB1, ABCC10, ABCC11 ve ABCG2) üzerinde önemli etkilere sahip olduğu gösterilmiştir. Ayrıca bileşiklerin apoptoz mekanizması genleri ve HSP’ler üzerinde önemli etkilere sahip olduğu tespit edilmiştir. Sonuç: Sonuçlarımız, sülfür içeren glisin imin türevlerinin, özellikle meme ve kolon kanserinde anti-kanser ajanı olabilecek önemli ve güçlü bir seçenek olabileceğini göstermektedir.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Mesci S, YAZGAN B, Gul M, Yıldırım T (2022). Effects of Sulfur Containing Glycine Imine Derivatives Compounds on Multidrug Resistance Proteins (MRPs) and Apoptosis Mechanism in MCF-7 and DLD-1 Cell Lines. , 461 - 469. 10.14235/bas.galenos.2021.6306
Chicago	Mesci Seda,YAZGAN BURAK,Gul Melek,Yıldırım Tuba Effects of Sulfur Containing Glycine Imine Derivatives Compounds on Multidrug Resistance Proteins (MRPs) and Apoptosis Mechanism in MCF-7 and DLD-1 Cell Lines. (2022): 461 - 469. 10.14235/bas.galenos.2021.6306
MLA	Mesci Seda,YAZGAN BURAK,Gul Melek,Yıldırım Tuba Effects of Sulfur Containing Glycine Imine Derivatives Compounds on Multidrug Resistance Proteins (MRPs) and Apoptosis Mechanism in MCF-7 and DLD-1 Cell Lines. , 2022, ss.461 - 469. 10.14235/bas.galenos.2021.6306
AMA	Mesci S,YAZGAN B,Gul M,Yıldırım T Effects of Sulfur Containing Glycine Imine Derivatives Compounds on Multidrug Resistance Proteins (MRPs) and Apoptosis Mechanism in MCF-7 and DLD-1 Cell Lines. . 2022; 461 - 469. 10.14235/bas.galenos.2021.6306
Vancouver	Mesci S,YAZGAN B,Gul M,Yıldırım T Effects of Sulfur Containing Glycine Imine Derivatives Compounds on Multidrug Resistance Proteins (MRPs) and Apoptosis Mechanism in MCF-7 and DLD-1 Cell Lines. . 2022; 461 - 469. 10.14235/bas.galenos.2021.6306
IEEE	Mesci S,YAZGAN B,Gul M,Yıldırım T "Effects of Sulfur Containing Glycine Imine Derivatives Compounds on Multidrug Resistance Proteins (MRPs) and Apoptosis Mechanism in MCF-7 and DLD-1 Cell Lines." , ss.461 - 469, 2022. 10.14235/bas.galenos.2021.6306
ISNAD	Mesci, Seda vd. "Effects of Sulfur Containing Glycine Imine Derivatives Compounds on Multidrug Resistance Proteins (MRPs) and Apoptosis Mechanism in MCF-7 and DLD-1 Cell Lines". (2022), 461-469. https://doi.org/10.14235/bas.galenos.2021.6306

APA	Mesci S, YAZGAN B, Gul M, Yıldırım T (2022). Effects of Sulfur Containing Glycine Imine Derivatives Compounds on Multidrug Resistance Proteins (MRPs) and Apoptosis Mechanism in MCF-7 and DLD-1 Cell Lines. Bezmiâlem Science, 10(4), 461 - 469. 10.14235/bas.galenos.2021.6306
Chicago	Mesci Seda,YAZGAN BURAK,Gul Melek,Yıldırım Tuba Effects of Sulfur Containing Glycine Imine Derivatives Compounds on Multidrug Resistance Proteins (MRPs) and Apoptosis Mechanism in MCF-7 and DLD-1 Cell Lines. Bezmiâlem Science 10, no.4 (2022): 461 - 469. 10.14235/bas.galenos.2021.6306
MLA	Mesci Seda,YAZGAN BURAK,Gul Melek,Yıldırım Tuba Effects of Sulfur Containing Glycine Imine Derivatives Compounds on Multidrug Resistance Proteins (MRPs) and Apoptosis Mechanism in MCF-7 and DLD-1 Cell Lines. Bezmiâlem Science, vol.10, no.4, 2022, ss.461 - 469. 10.14235/bas.galenos.2021.6306
AMA	Mesci S,YAZGAN B,Gul M,Yıldırım T Effects of Sulfur Containing Glycine Imine Derivatives Compounds on Multidrug Resistance Proteins (MRPs) and Apoptosis Mechanism in MCF-7 and DLD-1 Cell Lines. Bezmiâlem Science. 2022; 10(4): 461 - 469. 10.14235/bas.galenos.2021.6306
Vancouver	Mesci S,YAZGAN B,Gul M,Yıldırım T Effects of Sulfur Containing Glycine Imine Derivatives Compounds on Multidrug Resistance Proteins (MRPs) and Apoptosis Mechanism in MCF-7 and DLD-1 Cell Lines. Bezmiâlem Science. 2022; 10(4): 461 - 469. 10.14235/bas.galenos.2021.6306
IEEE	Mesci S,YAZGAN B,Gul M,Yıldırım T "Effects of Sulfur Containing Glycine Imine Derivatives Compounds on Multidrug Resistance Proteins (MRPs) and Apoptosis Mechanism in MCF-7 and DLD-1 Cell Lines." Bezmiâlem Science, 10, ss.461 - 469, 2022. 10.14235/bas.galenos.2021.6306
ISNAD	Mesci, Seda vd. "Effects of Sulfur Containing Glycine Imine Derivatives Compounds on Multidrug Resistance Proteins (MRPs) and Apoptosis Mechanism in MCF-7 and DLD-1 Cell Lines". Bezmiâlem Science 10/4 (2022), 461-469. https://doi.org/10.14235/bas.galenos.2021.6306