HOW DO THE GENETIC VARIANTS OF MMP 9 AND TIMP 1 AND VITAMIN D AFFECT CHRONIC RHINOSINUSITIS WITH NASAL POLYPOSIS?

Çomoğlu, Şenol; Aydemir, Levent; Keles Turel, Meryem Nesil; Ertugrul, Baris; Bozacı, Meltem; KASARCİ, GOKSU; Gelincik, Aslı; Cakmakoglu, Bedia

doi:10.26650/IUITFD.1210739

HOW DO THE GENETIC VARIANTS OF MMP 9 AND TIMP 1 AND VITAMIN D AFFECT CHRONIC RHINOSINUSITIS WITH NASAL POLYPOSIS?

Meltem BOZACI KILIÇOĞLU, (Istanbul University, Istanbul Faculty of Medicine, Department of Otorhinolaryngology, Division of Head and Neck Surgery, Istanbul, Turkiye)

Barış ERTUĞRUL, (Istanbul University, Aziz Sancar Institute of Experimental Medicine, Department of Molecular Medicine, Istanbul, Turkiye)

Goksu KAŞARCI, (Istanbul University, Aziz Sancar Institute of Experimental Medicine, Department of Molecular Medicine, Istanbul, Turkiye)

Şenol ÇOMOĞLU, (Istanbul University, Istanbul Faculty of Medicine, Department of Otorhinolaryngology, Division of Head and Neck Surgery, Istanbul, Turkiye)

Levent AYDEMİR, (Istanbul University, Istanbul Faculty of Medicine, Department of Otorhinolaryngology, Division of Head and Neck Surgery, Istanbul, Turkiye)

Meryem Nesil KELEŞ TÜREL, (Istanbul University, Istanbul Faculty of Medicine, Department of Otorhinolaryngology, Division of Head and Neck Surgery, Istanbul, Turkiye)

Aslı GELİNCİK, (Istanbul University, Istanbul Faculty of Medicine, Department of Internal Medicine, Division of Allergy and Immunology, Istanbul, Turkiye)

Bedia ÇAKMAKOĞLU (Istanbul University, Aziz Sancar Institute of Experimental Medicine, Department of Molecular Medicine, Istanbul, Turkiye)

İstanbul Tıp Fakültesi Dergisi

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Yıl: 2023 Cilt: 86 Sayı: 2 Sayfa Aralığı: 152 - 158 Metin Dili: İngilizce DOI: 10.26650/IUITFD.1210739 İndeks Tarihi: 10-05-2023

HOW DO THE GENETIC VARIANTS OF MMP 9 AND TIMP 1 AND VITAMIN D AFFECT CHRONIC RHINOSINUSITIS WITH NASAL POLYPOSIS?

Öz:

Objective: Chronic rhinosinusitis with nasal polyposis (CRSwNP) is a multifactorial disease in which the sinus and nasal mucosa are affected. We investigated the relationship between the genetic polymorphism of MMP9 (rs17576) and TIMP1 (rs48498) and serum levels, and we wanted to investigate the effect of MMP9 and TIMP1 genotypes on Vitamin serum levels in CRSwNP with and without Aspirin-Exacerbated Respiratory Disease (AERD). Materials and Methods: This study consisted of 99 patients with CRSwNP with AERD and CRSwNP without AERD. The RTPCR method and ELISA were used in this study. ELISA was used for MMP9 and TIMP1 serum levels and Vitamin D levels. Results: Serum levels of MMP9 were markedly higher in AG genotype than GG genotype in CRSwNP with AERD. Serum levels of MMP9 were statistically higher in carrying of A (+) allele in CRSwNP with AERD than in not carrying of A (+) allele. In addition, serum levels of Vitamin D were discovered significantly lower in TIMP1 CT genotype in comparison to CC and TT genotypes in all of CRSwNP. Conclusion: The rising of MMP9 serum levels may be a marker of chronic inflammation or they may be a significant factor in the resistance to diseases. This study may lead to a further understanding of disease severity in CRSwNP with AERD.

Anahtar Kelime:

MMP 9 VE TIMP 1 GENETİK VARYANTLARI VE VİTAMİN D NAZAL POLİPOZİSLİ KRONİK SİNÜZİTTE NASIL ETKİLİDİR?

Öz:

Amaç: Nazal polipli kronik rinosinüzit sinüs ve nazal mukozanın etkilendiği multifaktöriyel bir hastalıktır. MMP9 (rs17576) ve TIMP1 (rs 4898) genetik polimorfizmleri ile serum düzeyi arasındaki ilişki ve MMP9 ve TIMP1 genotipleri ve Vitamin D arasındaki bağlantının gösterilmesini araştırdık. Gereç ve Yöntem: Bu çalışma, aspirinle alevlenen solunum hastalığı olan veya olmayan nazal polipozisli kronik rinosinüzitli 99 hastadan oluşmaktadır. RT-PCR ve ELISA metodu kullanılmıştır. MMP9, TIMP1 ve Vitamin D serum seviyeleri için ELISA metodu kullanılmıştır. Bulgular: Aspirinle alevlenen solunum hastalığı eşlik eden nazal polipozisli kronik sinüzit hastalarında MMP9 serum seviyesi, AG genotipli hastalarda GG genotipli hastalara kıyasla daha yüksek seviyededir. Aynı zamanda bu hasta grubunda MMP9 serum seviyesi, A (+) alleli taşıyan grupta A (+) alleli taşımayan gruba göre daha yüksek seviyededir. Buna ek olarak TIMP1 CT genotipine sahip olanlar, TIMP1 CC ve TT genotipleri ile kıyaslandığında Vitamin D serum seviyesinin anlamlı olarak düşük olduğu gösterilmiştir. Sonuç: MMP9 serum düzeylerinin yükselmesi, kronik inflamasyonun bir belirteci olabilir veya hastalık direncinde önemli bir faktör olabilir. Bu çalışma, aspirinle alevlenen solunum hastalığı olan nazal polipozisli kronik rinosinüzitte hastalık şiddetinin daha iyi anlaşılmasına yardımcı olabilir.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Bozacı M, Ertugrul B, KASARCİ G, Çomoğlu Ş, Aydemir L, Keles Turel M, Gelincik A, Cakmakoglu B (2023). HOW DO THE GENETIC VARIANTS OF MMP 9 AND TIMP 1 AND VITAMIN D AFFECT CHRONIC RHINOSINUSITIS WITH NASAL POLYPOSIS?. , 152 - 158. 10.26650/IUITFD.1210739
Chicago	Bozacı Meltem,Ertugrul Baris,KASARCİ GOKSU,Çomoğlu Şenol,Aydemir Levent,Keles Turel Meryem Nesil,Gelincik Aslı,Cakmakoglu Bedia HOW DO THE GENETIC VARIANTS OF MMP 9 AND TIMP 1 AND VITAMIN D AFFECT CHRONIC RHINOSINUSITIS WITH NASAL POLYPOSIS?. (2023): 152 - 158. 10.26650/IUITFD.1210739
MLA	Bozacı Meltem,Ertugrul Baris,KASARCİ GOKSU,Çomoğlu Şenol,Aydemir Levent,Keles Turel Meryem Nesil,Gelincik Aslı,Cakmakoglu Bedia HOW DO THE GENETIC VARIANTS OF MMP 9 AND TIMP 1 AND VITAMIN D AFFECT CHRONIC RHINOSINUSITIS WITH NASAL POLYPOSIS?. , 2023, ss.152 - 158. 10.26650/IUITFD.1210739
AMA	Bozacı M,Ertugrul B,KASARCİ G,Çomoğlu Ş,Aydemir L,Keles Turel M,Gelincik A,Cakmakoglu B HOW DO THE GENETIC VARIANTS OF MMP 9 AND TIMP 1 AND VITAMIN D AFFECT CHRONIC RHINOSINUSITIS WITH NASAL POLYPOSIS?. . 2023; 152 - 158. 10.26650/IUITFD.1210739
Vancouver	Bozacı M,Ertugrul B,KASARCİ G,Çomoğlu Ş,Aydemir L,Keles Turel M,Gelincik A,Cakmakoglu B HOW DO THE GENETIC VARIANTS OF MMP 9 AND TIMP 1 AND VITAMIN D AFFECT CHRONIC RHINOSINUSITIS WITH NASAL POLYPOSIS?. . 2023; 152 - 158. 10.26650/IUITFD.1210739
IEEE	Bozacı M,Ertugrul B,KASARCİ G,Çomoğlu Ş,Aydemir L,Keles Turel M,Gelincik A,Cakmakoglu B "HOW DO THE GENETIC VARIANTS OF MMP 9 AND TIMP 1 AND VITAMIN D AFFECT CHRONIC RHINOSINUSITIS WITH NASAL POLYPOSIS?." , ss.152 - 158, 2023. 10.26650/IUITFD.1210739
ISNAD	Bozacı, Meltem vd. "HOW DO THE GENETIC VARIANTS OF MMP 9 AND TIMP 1 AND VITAMIN D AFFECT CHRONIC RHINOSINUSITIS WITH NASAL POLYPOSIS?". (2023), 152-158. https://doi.org/10.26650/IUITFD.1210739

APA	Bozacı M, Ertugrul B, KASARCİ G, Çomoğlu Ş, Aydemir L, Keles Turel M, Gelincik A, Cakmakoglu B (2023). HOW DO THE GENETIC VARIANTS OF MMP 9 AND TIMP 1 AND VITAMIN D AFFECT CHRONIC RHINOSINUSITIS WITH NASAL POLYPOSIS?. İstanbul Tıp Fakültesi Dergisi, 86(2), 152 - 158. 10.26650/IUITFD.1210739
Chicago	Bozacı Meltem,Ertugrul Baris,KASARCİ GOKSU,Çomoğlu Şenol,Aydemir Levent,Keles Turel Meryem Nesil,Gelincik Aslı,Cakmakoglu Bedia HOW DO THE GENETIC VARIANTS OF MMP 9 AND TIMP 1 AND VITAMIN D AFFECT CHRONIC RHINOSINUSITIS WITH NASAL POLYPOSIS?. İstanbul Tıp Fakültesi Dergisi 86, no.2 (2023): 152 - 158. 10.26650/IUITFD.1210739
MLA	Bozacı Meltem,Ertugrul Baris,KASARCİ GOKSU,Çomoğlu Şenol,Aydemir Levent,Keles Turel Meryem Nesil,Gelincik Aslı,Cakmakoglu Bedia HOW DO THE GENETIC VARIANTS OF MMP 9 AND TIMP 1 AND VITAMIN D AFFECT CHRONIC RHINOSINUSITIS WITH NASAL POLYPOSIS?. İstanbul Tıp Fakültesi Dergisi, vol.86, no.2, 2023, ss.152 - 158. 10.26650/IUITFD.1210739
AMA	Bozacı M,Ertugrul B,KASARCİ G,Çomoğlu Ş,Aydemir L,Keles Turel M,Gelincik A,Cakmakoglu B HOW DO THE GENETIC VARIANTS OF MMP 9 AND TIMP 1 AND VITAMIN D AFFECT CHRONIC RHINOSINUSITIS WITH NASAL POLYPOSIS?. İstanbul Tıp Fakültesi Dergisi. 2023; 86(2): 152 - 158. 10.26650/IUITFD.1210739
Vancouver	Bozacı M,Ertugrul B,KASARCİ G,Çomoğlu Ş,Aydemir L,Keles Turel M,Gelincik A,Cakmakoglu B HOW DO THE GENETIC VARIANTS OF MMP 9 AND TIMP 1 AND VITAMIN D AFFECT CHRONIC RHINOSINUSITIS WITH NASAL POLYPOSIS?. İstanbul Tıp Fakültesi Dergisi. 2023; 86(2): 152 - 158. 10.26650/IUITFD.1210739
IEEE	Bozacı M,Ertugrul B,KASARCİ G,Çomoğlu Ş,Aydemir L,Keles Turel M,Gelincik A,Cakmakoglu B "HOW DO THE GENETIC VARIANTS OF MMP 9 AND TIMP 1 AND VITAMIN D AFFECT CHRONIC RHINOSINUSITIS WITH NASAL POLYPOSIS?." İstanbul Tıp Fakültesi Dergisi, 86, ss.152 - 158, 2023. 10.26650/IUITFD.1210739
ISNAD	Bozacı, Meltem vd. "HOW DO THE GENETIC VARIANTS OF MMP 9 AND TIMP 1 AND VITAMIN D AFFECT CHRONIC RHINOSINUSITIS WITH NASAL POLYPOSIS?". İstanbul Tıp Fakültesi Dergisi 86/2 (2023), 152-158. https://doi.org/10.26650/IUITFD.1210739