Effect of probiotics on aspirin-induced gastric mucosal lesions

Amaç: Aspirinle oluşturulan gastrik mukozal hasarın önlenmesinde 13 farklı bakteri içeren karışım probiyotiğin rolünü araştırmaktır.Yöntem: Kırk rat 4 gruba ayrıldı; normal kontrol, aspirin, probiyotik kontrol ve probiyotik- aspirin. Normal kontrol ve aspirin gruplarına 0.2 mL gavaj olarak 14 gün süt tozu, probiyotik kontrol ve probiyotik-aspirin gruplarına 0.2 mL probiyotik karışımı (1,3 x 10x10 cfu/mL) süt tozu içinde süspansiyon yapılarak gavaj olarak 14 gün verildi. On beşinci gün mide lezyonu oluşturmak için aspirin ve probiyotik-aspirin gruplarına aspirin (200 mg/kg), normal kontrol ve probiyotik kontrol grublarına salin solüsyonu verildi. Bulgular: Tedavi öncesi probiyotik karışımı, aspirin ile oluşturulan mide hasar skorunu azalttı (4.50 ± 0.43 & 2.60 ±0.40, p<0.01) ve aspirinin oluşturduğu proinflamatuvar sitokin seviyelerini düşürne eğilimindeydi (p>0.05). Karışım probiyotiğin, midede sIgA düzeylerini yaklaşık 7.5 kat arttırdığı ve aspirinin neden olduğu mide mukozasında yüksek MDA düzeylerini anlamlı olarak azalttığı tespit edildi (p<0.001). Ayrıca tedavi öncesi uygulanan karışım probiyotik, mide mukozasında aspirinin düşürdüğü mast hücre sayısını yükseltti. Sonuç: Tedavi öncesi verilen probiyotik karışımı, aspirinin neden olduğu mide lezyonlarını, lipid peroksidasyonunu azaltarak, mukozal sIgA üretimini arttırarak ve mide mukozasında mukozal mast hücre degranülasyonunu stabilize ederek azaltır.

Anahtar Kelime:

Konular: Cerrahi

Aspirinle oluşturulan gastrik mukozal lezyonlarda probiyotiklerin etkisi

Öz:

Background/aims: We aimed to investigate the role of a probiotic mixture, including 13 different bacteria, in the prevention of aspirin- induced gastric mucosal injury. Methods: Forty rats were allocated into 4 groups: normal control, aspirin, probiotic control, and probiotic plus aspirin. Normal control and aspirin groups received 0.2 ml of skim milk by daily gavage for 14 days. Probiotic control and probiotic plus aspirin groups were administered 0.2 ml/day of probiotic mixture (1.3 x 1010 cfu/ml) suspended in skim milk by daily gavage for 14 days. On day 15, gastric lesions were induced by administration of aspirin (200 mg/kg) in the aspirin and probiotic plus aspirin groups. Normal control and probiotic control groups were given saline. Results: Pretreatment with probiotic mixture reduced aspirin-induced gastric damage scores (4.50 ± 0.43 and 2.60 ± 0.40, p<0.01) and exerted tendency of downregulation of proinflammatory cytokines elicited by aspirin (p>0.05). We also found that the probiotic mixture increased sIgA production approximately 7.5-fold in the stomach, and significantly reduced the malondialdehyde (MDA) increase in the gastric mucosa elicited by aspirin (p<0.001). Additionally, pretreatment with the probiotic mixture alleviated aspirin-induced reduction of mast cell count in the gastric mucosa. Conclusions: Probiotic mixture pretreatment attenuates the aspirin-induced gastric lesions by reducing the lipid peroxidation, enhancing mucosal sIgA production, and stabilizing mucosal mast cell degranulation into the gastric mucosa.

Anahtar Kelime:

Konular: Cerrahi

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	ŞENOL A, İŞLER M, KARAHAN A, KILIÇ G, KULEAŞAN H, Gören İ, SARITAŞ Ü, Kaya S, ÇİRİŞ İ, AKTÜRK O, ARIDOĞAN B, DEMİRİN H, ÇAKMAKÇI L (2011). Effect of probiotics on aspirin-induced gastric mucosal lesions. , 18 - 26.
Chicago	ŞENOL Altuğ,İŞLER Mehmet,KARAHAN Aynur G.,KILIÇ Gülden B.,KULEAŞAN HAKAN,Gören İbrahim,SARITAŞ Ülkü,Kaya Selçuk,ÇİRİŞ İbrahim Metin,AKTÜRK Onur,ARIDOĞAN Buket C.,DEMİRİN Hilmi,ÇAKMAKÇI Lütfü M. Effect of probiotics on aspirin-induced gastric mucosal lesions. (2011): 18 - 26.
MLA	ŞENOL Altuğ,İŞLER Mehmet,KARAHAN Aynur G.,KILIÇ Gülden B.,KULEAŞAN HAKAN,Gören İbrahim,SARITAŞ Ülkü,Kaya Selçuk,ÇİRİŞ İbrahim Metin,AKTÜRK Onur,ARIDOĞAN Buket C.,DEMİRİN Hilmi,ÇAKMAKÇI Lütfü M. Effect of probiotics on aspirin-induced gastric mucosal lesions. , 2011, ss.18 - 26.
AMA	ŞENOL A,İŞLER M,KARAHAN A,KILIÇ G,KULEAŞAN H,Gören İ,SARITAŞ Ü,Kaya S,ÇİRİŞ İ,AKTÜRK O,ARIDOĞAN B,DEMİRİN H,ÇAKMAKÇI L Effect of probiotics on aspirin-induced gastric mucosal lesions. . 2011; 18 - 26.
Vancouver	ŞENOL A,İŞLER M,KARAHAN A,KILIÇ G,KULEAŞAN H,Gören İ,SARITAŞ Ü,Kaya S,ÇİRİŞ İ,AKTÜRK O,ARIDOĞAN B,DEMİRİN H,ÇAKMAKÇI L Effect of probiotics on aspirin-induced gastric mucosal lesions. . 2011; 18 - 26.
IEEE	ŞENOL A,İŞLER M,KARAHAN A,KILIÇ G,KULEAŞAN H,Gören İ,SARITAŞ Ü,Kaya S,ÇİRİŞ İ,AKTÜRK O,ARIDOĞAN B,DEMİRİN H,ÇAKMAKÇI L "Effect of probiotics on aspirin-induced gastric mucosal lesions." , ss.18 - 26, 2011.
ISNAD	ŞENOL, Altuğ vd. "Effect of probiotics on aspirin-induced gastric mucosal lesions". (2011), 18-26.

APA	ŞENOL A, İŞLER M, KARAHAN A, KILIÇ G, KULEAŞAN H, Gören İ, SARITAŞ Ü, Kaya S, ÇİRİŞ İ, AKTÜRK O, ARIDOĞAN B, DEMİRİN H, ÇAKMAKÇI L (2011). Effect of probiotics on aspirin-induced gastric mucosal lesions. Turkish Journal of Gastroenterology, 22(1), 18 - 26.
Chicago	ŞENOL Altuğ,İŞLER Mehmet,KARAHAN Aynur G.,KILIÇ Gülden B.,KULEAŞAN HAKAN,Gören İbrahim,SARITAŞ Ülkü,Kaya Selçuk,ÇİRİŞ İbrahim Metin,AKTÜRK Onur,ARIDOĞAN Buket C.,DEMİRİN Hilmi,ÇAKMAKÇI Lütfü M. Effect of probiotics on aspirin-induced gastric mucosal lesions. Turkish Journal of Gastroenterology 22, no.1 (2011): 18 - 26.
MLA	ŞENOL Altuğ,İŞLER Mehmet,KARAHAN Aynur G.,KILIÇ Gülden B.,KULEAŞAN HAKAN,Gören İbrahim,SARITAŞ Ülkü,Kaya Selçuk,ÇİRİŞ İbrahim Metin,AKTÜRK Onur,ARIDOĞAN Buket C.,DEMİRİN Hilmi,ÇAKMAKÇI Lütfü M. Effect of probiotics on aspirin-induced gastric mucosal lesions. Turkish Journal of Gastroenterology, vol.22, no.1, 2011, ss.18 - 26.
AMA	ŞENOL A,İŞLER M,KARAHAN A,KILIÇ G,KULEAŞAN H,Gören İ,SARITAŞ Ü,Kaya S,ÇİRİŞ İ,AKTÜRK O,ARIDOĞAN B,DEMİRİN H,ÇAKMAKÇI L Effect of probiotics on aspirin-induced gastric mucosal lesions. Turkish Journal of Gastroenterology. 2011; 22(1): 18 - 26.
Vancouver	ŞENOL A,İŞLER M,KARAHAN A,KILIÇ G,KULEAŞAN H,Gören İ,SARITAŞ Ü,Kaya S,ÇİRİŞ İ,AKTÜRK O,ARIDOĞAN B,DEMİRİN H,ÇAKMAKÇI L Effect of probiotics on aspirin-induced gastric mucosal lesions. Turkish Journal of Gastroenterology. 2011; 22(1): 18 - 26.
IEEE	ŞENOL A,İŞLER M,KARAHAN A,KILIÇ G,KULEAŞAN H,Gören İ,SARITAŞ Ü,Kaya S,ÇİRİŞ İ,AKTÜRK O,ARIDOĞAN B,DEMİRİN H,ÇAKMAKÇI L "Effect of probiotics on aspirin-induced gastric mucosal lesions." Turkish Journal of Gastroenterology, 22, ss.18 - 26, 2011.
ISNAD	ŞENOL, Altuğ vd. "Effect of probiotics on aspirin-induced gastric mucosal lesions". Turkish Journal of Gastroenterology 22/1 (2011), 18-26.