Adli DNA Analizlerinde Güncel Moleküler Genetik Gelişmeler

tural, sengul; TEKCAN, ESRA

doi:10.5505/vtd.2023.30633

Adli DNA Analizlerinde Güncel Moleküler Genetik Gelişmeler

Esra TEKCAN, (Ondokuz Mayız Üniversitesi, Tıp Fakültesi, Tıbbi Biyoloji AD, Samsun, TÜRKİYE)

Şengül TURAL (Ondokuz Mayız Üniversitesi, Tıp Fakültesi, Tıbbi Biyoloji AD, Samsun, TÜRKİYE)

Van Tıp Dergisi

26 11

Yıl: 2023 Cilt: 30 Sayı: 2 Sayfa Aralığı: 217 - 222 Metin Dili: Türkçe DOI: 10.5505/vtd.2023.30633 İndeks Tarihi: 12-05-2023

Adli DNA Analizlerinde Güncel Moleküler Genetik Gelişmeler

Öz:

Adli biyolojik delillerden elde edilen DNA analizi olayla ilişkili failin belirlenmesinde güçlü kan ıtlar sağlamaktadır. K ısa ardışık tekrar bölgelerindeki (STR) tekrar sayısının ki şiler arası farklılık göstermesi ve bu bölgedelerdeki mutasyon oranının az olmas ı donörün kimliklendirilmesinde STR’leri genetik markır olarak tercih edilir hale getirmiştir. Fakat adli biyolojik materyaldeki DNA miktarının çok düşük olması ya da DNA’nın analize izin vermeyecek derecede bozulması, adli genetik yöntemlerde sıklıkla kullanılan k ısa ardışık tekrar bölgelerinin belirlenememesine neden olabilir. Şüpheli bir durumda, STR profili ile bir eşleşme olmadığında, numunenin donörünün belirlenmesine yardımcı olabilecek herhangi bir bilgi çok değerli olacaktır. Bu nedenle adli genetikte, analizi güç olan biyolojik örneklerin ait oldu ğu kimliğin teşhis edilmesinde, adli DNA fenotipleme ile donörün ya şı, saç ve göz rengi gibi fiziksel görünümü hakk ında ek bilgilerin çıkarılmasında, ayr ıca vücut sıvısı ve doku tipi tayini için mRNA ve miRNA analizlerini içeren, genetik ve epigenetik alanda yeni güncel moleküler metodlar gelişmeye ba şlamıştır. Bu derlemede, STR’lerin ve diğer markırların analizine büyük ölçüde masif paralel dizilemenin (massivelly parallel sequencing, MPS) uygulanmas ı, birden fazla ki şiye ait genetik materyal içeren karışım DNA profillerinin yorumlanmasındaki gelişmeler, vücut sıvılarının tanımlanması için RNA profillerinin belirlenmesi ve metilasyon profillerinin incelenmesi gibi epigenetik yöntemlerin de dahil edilmesiyle ilgili fenotipin belirlenmesinde, analiz potansiyelinin en üst seviyeye çıkarılması için bu alandaki son geli şmeler gözden geçirilmiştir.

Anahtar Kelime:

Current Molecular Genetic Developments in Forensic DNA Analysis

Öz:

DNA analysis obtained from forensic biological evidence provides strong evidence in identifying the perpetrator associated with the incident. The interindividual difference in the number of repeats in short tandem repeat (STR) regions and the low mutation rate in these regions have made STRs preferred as genetic markers for donor identification. However, if the amount of DNA in the fore nsic biological material is too low or the DNA is corrupted to such an extent that it does not allow analysis, the short tand em repeat regions, which are frequently used in forensic genetic methods, cannot be determined. In the case of doubt, when there is no match to the STR profile, any information that can assist in identifying the sample's donor would be invaluable. Therefore, in the forensic genetics, in the diagnos is of the identity of biological samples that are difficult to analyze, determining the additional information about the physical appearence of the donor such as age, hair and eyes colours with forensic DNA phenotyping, also such as body fluid and tissue type determination with mRNA and miRNA analyses, new current molecular methods have started to develop in the genetic and epigenetic field. In this review, for analysis of STRs and other markers we review recent advances in this field to maximize the analysis potential in identifying the phenotype of interest, largely through the application of massive pa rallel sequencing (MPS), developments in the interpretation of mixture DNA profiles containing genetic material of more than one person, RNA profiling for body fluid identification, and inclusion of epigenetic methods such as examination of methylation profiles.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	TEKCAN E, tural s (2023). Adli DNA Analizlerinde Güncel Moleküler Genetik Gelişmeler. , 217 - 222. 10.5505/vtd.2023.30633
Chicago	TEKCAN ESRA,tural sengul Adli DNA Analizlerinde Güncel Moleküler Genetik Gelişmeler. (2023): 217 - 222. 10.5505/vtd.2023.30633
MLA	TEKCAN ESRA,tural sengul Adli DNA Analizlerinde Güncel Moleküler Genetik Gelişmeler. , 2023, ss.217 - 222. 10.5505/vtd.2023.30633
AMA	TEKCAN E,tural s Adli DNA Analizlerinde Güncel Moleküler Genetik Gelişmeler. . 2023; 217 - 222. 10.5505/vtd.2023.30633
Vancouver	TEKCAN E,tural s Adli DNA Analizlerinde Güncel Moleküler Genetik Gelişmeler. . 2023; 217 - 222. 10.5505/vtd.2023.30633
IEEE	TEKCAN E,tural s "Adli DNA Analizlerinde Güncel Moleküler Genetik Gelişmeler." , ss.217 - 222, 2023. 10.5505/vtd.2023.30633
ISNAD	TEKCAN, ESRA - tural, sengul. "Adli DNA Analizlerinde Güncel Moleküler Genetik Gelişmeler". (2023), 217-222. https://doi.org/10.5505/vtd.2023.30633

APA	TEKCAN E, tural s (2023). Adli DNA Analizlerinde Güncel Moleküler Genetik Gelişmeler. Van Tıp Dergisi, 30(2), 217 - 222. 10.5505/vtd.2023.30633
Chicago	TEKCAN ESRA,tural sengul Adli DNA Analizlerinde Güncel Moleküler Genetik Gelişmeler. Van Tıp Dergisi 30, no.2 (2023): 217 - 222. 10.5505/vtd.2023.30633
MLA	TEKCAN ESRA,tural sengul Adli DNA Analizlerinde Güncel Moleküler Genetik Gelişmeler. Van Tıp Dergisi, vol.30, no.2, 2023, ss.217 - 222. 10.5505/vtd.2023.30633
AMA	TEKCAN E,tural s Adli DNA Analizlerinde Güncel Moleküler Genetik Gelişmeler. Van Tıp Dergisi. 2023; 30(2): 217 - 222. 10.5505/vtd.2023.30633
Vancouver	TEKCAN E,tural s Adli DNA Analizlerinde Güncel Moleküler Genetik Gelişmeler. Van Tıp Dergisi. 2023; 30(2): 217 - 222. 10.5505/vtd.2023.30633
IEEE	TEKCAN E,tural s "Adli DNA Analizlerinde Güncel Moleküler Genetik Gelişmeler." Van Tıp Dergisi, 30, ss.217 - 222, 2023. 10.5505/vtd.2023.30633
ISNAD	TEKCAN, ESRA - tural, sengul. "Adli DNA Analizlerinde Güncel Moleküler Genetik Gelişmeler". Van Tıp Dergisi 30/2 (2023), 217-222. https://doi.org/10.5505/vtd.2023.30633