The Effect of Concentration and Storage Time on Short-Term Storage of Ram Sperm
Yıl: 2022 Cilt: 28 Sayı: 6 Sayfa Aralığı: 681 - 690 Metin Dili: İngilizce DOI: 10.9775/kvfd.2022.27636 İndeks Tarihi: 15-05-2023
The Effect of Concentration and Storage Time on Short-Term Storage of Ram Sperm
Öz: The present study was conducted to observe the effect of different dilution rates on ram sperm motion characters (motility, progressivity, velocity and average of speed), head diameter, mitochondrial membrane potential (MMP) and live intact acrosome rate during liquid storage for 96 h at 5ºC. Normospermic semen samples that obtained from 4 adult rams were pooled. Pooled semen was split into two aliquots; Low Dilution Rate (LDR) (400 million) and High Dilution Rate (HDR) (20 million) motile spermatozoa per insemination dose/0.25 mL straw and were diluted with a skim milk-egg yolk-based extender. It was observed that the Total Motility (TM) and total progressivity of the LDR group decreased more rapidly than HDR group over time, in general. From the 12th h to the 96th h; the rapid progressivity (P<0.01), MMP (P<0.05), live sperm rates with intact acrosome (P<0.01) and straightness (STR) (P<0.001) of the HDR group results were statistically better than LDR. It was concluded that the spermatological properties examined during storage were damaged at different times and were affected by sperm concentration and also storage time.
Anahtar Kelime: Koç Spermasının Kısa Süreli Saklanmasına Konsantrasyonun ve Muhafaza Süresinin Etkisi
Öz: Bu çalışma, koç spermasının 5ºC’de 96 saat süre ile saklanmasında farklı sulandırma oranlarının sperm hareketleri ile ilişkili (motilite, progressivite ve ortalama hız) parametreleri, baş çapı, mitokondriyal membran potansiyeli ve canlı sağlam akrozoma sahip spermatozoa oranlarına etkilerinin ortaya konulması amacıyla gerçekleştirildi. Dört baş erişkin koçtan elde edilen normospermik özelliğe sahip ejekulatlar birleştirildi. Birleştirilen ejekulatlar ikiye bölünerek; Düşük Dilüsyon Oranı (LDR) (400 milyon) ve Yüksek Dilüsyon Oranı (20 milyon), motil spermatozoa tohumlama dozunda/0.25 mL payet olacak şekilde yağsız süt tozu yumurta sarısı bazlı sulandırıcı ile sulandırıldı. Genel olarak Düşük sulandırma oranı (LDR) grubunda TM ve progressivitenin Yüksek sulandırma oranı (HDR) grubuna göre daha hızlı azaldığı gözlemlendi. On ikinci saatten 96. saate kadar ki saklama süresinde, HDR grubunda elde edilen hızlı progresif motilitenin (P<0.01), mitokondriyal membran potansiyelinin (P<0.05) ve sağlam akrozoma sahip canlı spermatozoa oranının (P<0.01), doğrusallığın (STR) (P<0.001) LDR grubuna göre istatistiksel olarak daha yüksek olduğu gözlemlendi. Saklama süresince incelenen spermatolojik özelliklerin farklı zamanlarda zarar gördüğü, ayrıca sperm konsantrasyonu ve saklama süresinden de etkilendiği sonucuna varıldı.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | DAYANIKLI C, ŞENGÜL E, Bülbül B, ustuner b, NUR Z (2022). The Effect of Concentration and Storage Time on Short-Term Storage of Ram Sperm. , 681 - 690. 10.9775/kvfd.2022.27636 |
| Chicago | DAYANIKLI CEMAL,ŞENGÜL EBRU,Bülbül Bülent,ustuner burcu,NUR ZEKARIYA The Effect of Concentration and Storage Time on Short-Term Storage of Ram Sperm. (2022): 681 - 690. 10.9775/kvfd.2022.27636 |
| MLA | DAYANIKLI CEMAL,ŞENGÜL EBRU,Bülbül Bülent,ustuner burcu,NUR ZEKARIYA The Effect of Concentration and Storage Time on Short-Term Storage of Ram Sperm. , 2022, ss.681 - 690. 10.9775/kvfd.2022.27636 |
| AMA | DAYANIKLI C,ŞENGÜL E,Bülbül B,ustuner b,NUR Z The Effect of Concentration and Storage Time on Short-Term Storage of Ram Sperm. . 2022; 681 - 690. 10.9775/kvfd.2022.27636 |
| Vancouver | DAYANIKLI C,ŞENGÜL E,Bülbül B,ustuner b,NUR Z The Effect of Concentration and Storage Time on Short-Term Storage of Ram Sperm. . 2022; 681 - 690. 10.9775/kvfd.2022.27636 |
| IEEE | DAYANIKLI C,ŞENGÜL E,Bülbül B,ustuner b,NUR Z "The Effect of Concentration and Storage Time on Short-Term Storage of Ram Sperm." , ss.681 - 690, 2022. 10.9775/kvfd.2022.27636 |
| ISNAD | DAYANIKLI, CEMAL vd. "The Effect of Concentration and Storage Time on Short-Term Storage of Ram Sperm". (2022), 681-690. https://doi.org/10.9775/kvfd.2022.27636 |
| APA | DAYANIKLI C, ŞENGÜL E, Bülbül B, ustuner b, NUR Z (2022). The Effect of Concentration and Storage Time on Short-Term Storage of Ram Sperm. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 28(6), 681 - 690. 10.9775/kvfd.2022.27636 |
| Chicago | DAYANIKLI CEMAL,ŞENGÜL EBRU,Bülbül Bülent,ustuner burcu,NUR ZEKARIYA The Effect of Concentration and Storage Time on Short-Term Storage of Ram Sperm. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 28, no.6 (2022): 681 - 690. 10.9775/kvfd.2022.27636 |
| MLA | DAYANIKLI CEMAL,ŞENGÜL EBRU,Bülbül Bülent,ustuner burcu,NUR ZEKARIYA The Effect of Concentration and Storage Time on Short-Term Storage of Ram Sperm. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.28, no.6, 2022, ss.681 - 690. 10.9775/kvfd.2022.27636 |
| AMA | DAYANIKLI C,ŞENGÜL E,Bülbül B,ustuner b,NUR Z The Effect of Concentration and Storage Time on Short-Term Storage of Ram Sperm. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2022; 28(6): 681 - 690. 10.9775/kvfd.2022.27636 |
| Vancouver | DAYANIKLI C,ŞENGÜL E,Bülbül B,ustuner b,NUR Z The Effect of Concentration and Storage Time on Short-Term Storage of Ram Sperm. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2022; 28(6): 681 - 690. 10.9775/kvfd.2022.27636 |
| IEEE | DAYANIKLI C,ŞENGÜL E,Bülbül B,ustuner b,NUR Z "The Effect of Concentration and Storage Time on Short-Term Storage of Ram Sperm." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 28, ss.681 - 690, 2022. 10.9775/kvfd.2022.27636 |
| ISNAD | DAYANIKLI, CEMAL vd. "The Effect of Concentration and Storage Time on Short-Term Storage of Ram Sperm". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 28/6 (2022), 681-690. https://doi.org/10.9775/kvfd.2022.27636 |
