Associations Between MSTN/HaeIII Polymorphism and Reproductive and Growth Characteristics in Morkaraman Sheep
Yıl: 2022 Cilt: 28 Sayı: 6 Sayfa Aralığı: 717 - 722 Metin Dili: İngilizce DOI: 10.9775/kvfd.2022.27952 İndeks Tarihi: 15-05-2023
Associations Between MSTN/HaeIII Polymorphism and Reproductive and Growth Characteristics in Morkaraman Sheep
Öz: The myostatin gene inhibits skeletal muscle growth in advanced animals, and a mutation in the gene coding region increases muscle formation. Hence, it is accepted to be a candidate gene for the selection of some production traits. The objective of the current research was to examine the genotypes of the myostatin gene and reveal the associations between the genotypes and a number of traits, including birth weight, weaning weight, weaning age, average daily weight gain, and productivity, in 110 Morkaraman sheep. Genotypes were determined by the PCR-RFLP method using the HaeIII restriction enzyme, and the genotype frequencies were found to be 9%, 69%, and 22% for MM, Mm, and mm in the myostatin locus, respectively. The M allele frequency was 44%, whereas the m allele frequency was 56%. In the studied population, the myostatin locus was not in Hardy-Weinberg equilibrium. The association analysis revealed no statistically significant impact of the MSTN gene polymorphism in exon 3 on birth weight, weaning weight, and productivity (P>0.05) but found a significant effect on weaning age and average daily weight gain (P<0.05). As a result, the MSTN gene showed polymorphisms in Morkaraman sheep and can be regarded as a genetic marker for sheep selection according to the association analysis results.
Anahtar Kelime: Morkaraman Koyunlarında MSTN/HaeIII Polimorfizmi İle Dölverimi ve Büyüme Özellikleri Arasındaki İlişkiler
Öz: Miyostatin geni, gelişmiş hayvanlarda iskelet kası büyümesinin bir inhibitörüdür ve gen kodlama bölgesindeki bir mutasyon kas oluşumunu arttırır. Bu nedenle bazı verim özelliklerinin ıslahı için aday gen olarak kabul edilmektedir. Çalışmada, Morkaraman koyunlarından alınan genomik DNA örneklerinden MSTN/HaeIII gen polimorfizmine ait genotiplerin araştırılması, genotip ve alel frekanslarının dağılımının belirlenmesi ve genotipler ile doğum ağırlığı, sütten kesim ağırlığı, sütten kesim yaşı, ortalama günlük canlı ağırlık artışı ve dölverimi gibi bazı özellikler arasındaki ilişkilerin belirlenmesi amaçlanmıştır. Kayıtlı 110 Morkaraman koyunundan kan örnekleri alındı ve her bir örnekten DNA ekstrakte edildi. Genotipler, PCR-RFLP yöntemi ile myostatin geninin polimorfik ekson 3 bölgesi için HaeIII restriksiyon enzimi kullanılarak belirlendi. Sonuç olarak, miyostatin lokusunda MM, Mm ve mm için genotip frekansları sırasıyla %9, %69 ve %22 idi. M allel frekansı %44 ve m allel frekansı %56 idi. Popülasyonda miyostatin lokusu Hardy-Weinberg genetik dengesinde değildi. Sonuçlara göre MSTN geni ekson 3 polimorfizminin etkisi, doğum ağırlığı, sütten kesim ağırlığı ve dölverimi üzerine istatistiksel olarak önemli bulunamadı (P>0.05), ancak, sütten kesim yaşı ve ortalama günlük canlı ağırlık artışı üzerine etkisi önemliydi (P<0.05). Sonuç olarak, MSTN geni Morkaraman koyunlarında polimorfizm göstermiştir ve bu polimorfizm ıslah çalışmalarında genetik markör olarak kullanılabilir.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | SAYGILI E, TÜRKYILMAZ D, Ekinci k, Dağdelen Ü, Özdemir M, Esenbuga N, Kopuzlu S (2022). Associations Between MSTN/HaeIII Polymorphism and Reproductive and Growth Characteristics in Morkaraman Sheep. , 717 - 722. 10.9775/kvfd.2022.27952 |
| Chicago | SAYGILI ESRANUR,TÜRKYILMAZ DOĞAN,Ekinci kübra,Dağdelen Ülkü,Özdemir Memiş,Esenbuga Nurinisa,Kopuzlu Sinan Associations Between MSTN/HaeIII Polymorphism and Reproductive and Growth Characteristics in Morkaraman Sheep. (2022): 717 - 722. 10.9775/kvfd.2022.27952 |
| MLA | SAYGILI ESRANUR,TÜRKYILMAZ DOĞAN,Ekinci kübra,Dağdelen Ülkü,Özdemir Memiş,Esenbuga Nurinisa,Kopuzlu Sinan Associations Between MSTN/HaeIII Polymorphism and Reproductive and Growth Characteristics in Morkaraman Sheep. , 2022, ss.717 - 722. 10.9775/kvfd.2022.27952 |
| AMA | SAYGILI E,TÜRKYILMAZ D,Ekinci k,Dağdelen Ü,Özdemir M,Esenbuga N,Kopuzlu S Associations Between MSTN/HaeIII Polymorphism and Reproductive and Growth Characteristics in Morkaraman Sheep. . 2022; 717 - 722. 10.9775/kvfd.2022.27952 |
| Vancouver | SAYGILI E,TÜRKYILMAZ D,Ekinci k,Dağdelen Ü,Özdemir M,Esenbuga N,Kopuzlu S Associations Between MSTN/HaeIII Polymorphism and Reproductive and Growth Characteristics in Morkaraman Sheep. . 2022; 717 - 722. 10.9775/kvfd.2022.27952 |
| IEEE | SAYGILI E,TÜRKYILMAZ D,Ekinci k,Dağdelen Ü,Özdemir M,Esenbuga N,Kopuzlu S "Associations Between MSTN/HaeIII Polymorphism and Reproductive and Growth Characteristics in Morkaraman Sheep." , ss.717 - 722, 2022. 10.9775/kvfd.2022.27952 |
| ISNAD | SAYGILI, ESRANUR vd. "Associations Between MSTN/HaeIII Polymorphism and Reproductive and Growth Characteristics in Morkaraman Sheep". (2022), 717-722. https://doi.org/10.9775/kvfd.2022.27952 |
| APA | SAYGILI E, TÜRKYILMAZ D, Ekinci k, Dağdelen Ü, Özdemir M, Esenbuga N, Kopuzlu S (2022). Associations Between MSTN/HaeIII Polymorphism and Reproductive and Growth Characteristics in Morkaraman Sheep. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 28(6), 717 - 722. 10.9775/kvfd.2022.27952 |
| Chicago | SAYGILI ESRANUR,TÜRKYILMAZ DOĞAN,Ekinci kübra,Dağdelen Ülkü,Özdemir Memiş,Esenbuga Nurinisa,Kopuzlu Sinan Associations Between MSTN/HaeIII Polymorphism and Reproductive and Growth Characteristics in Morkaraman Sheep. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 28, no.6 (2022): 717 - 722. 10.9775/kvfd.2022.27952 |
| MLA | SAYGILI ESRANUR,TÜRKYILMAZ DOĞAN,Ekinci kübra,Dağdelen Ülkü,Özdemir Memiş,Esenbuga Nurinisa,Kopuzlu Sinan Associations Between MSTN/HaeIII Polymorphism and Reproductive and Growth Characteristics in Morkaraman Sheep. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.28, no.6, 2022, ss.717 - 722. 10.9775/kvfd.2022.27952 |
| AMA | SAYGILI E,TÜRKYILMAZ D,Ekinci k,Dağdelen Ü,Özdemir M,Esenbuga N,Kopuzlu S Associations Between MSTN/HaeIII Polymorphism and Reproductive and Growth Characteristics in Morkaraman Sheep. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2022; 28(6): 717 - 722. 10.9775/kvfd.2022.27952 |
| Vancouver | SAYGILI E,TÜRKYILMAZ D,Ekinci k,Dağdelen Ü,Özdemir M,Esenbuga N,Kopuzlu S Associations Between MSTN/HaeIII Polymorphism and Reproductive and Growth Characteristics in Morkaraman Sheep. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2022; 28(6): 717 - 722. 10.9775/kvfd.2022.27952 |
| IEEE | SAYGILI E,TÜRKYILMAZ D,Ekinci k,Dağdelen Ü,Özdemir M,Esenbuga N,Kopuzlu S "Associations Between MSTN/HaeIII Polymorphism and Reproductive and Growth Characteristics in Morkaraman Sheep." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 28, ss.717 - 722, 2022. 10.9775/kvfd.2022.27952 |
| ISNAD | SAYGILI, ESRANUR vd. "Associations Between MSTN/HaeIII Polymorphism and Reproductive and Growth Characteristics in Morkaraman Sheep". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 28/6 (2022), 717-722. https://doi.org/10.9775/kvfd.2022.27952 |
