TY  - JOUR
TI  - Reno-Protective Effects of Brusatol Against Renal Ischemia Reperfusion Injury
AB  - Objective: The aim of this study was to determine the potential beneficial effects of brusatol treatment on oxidative kidney injury induced by bilateral renal ischemia reperfusion (RIR) method. Material and Method: In the existing study, experimental animals were randomlyassigned to 4 groups as sham, RIR, Dimethyl Sulfoxide (DMSO) and brusatol groups. Sham group; the back region was opened by incision and then sutured but no ischemia reperfusion (IR) model was established. In RIR group, 1 hour of ischemia following 24 hours of reperfusion was formed. In DMSO group, 0.3 ml, 1% DMSO was administered intraperitoneally. Then IR model was carried out as told in RIR group. In brusatol group, brusatol was applied intraperitoneally as 0.5 mg/ml for each rat every other days for 10 days before the experiment. The last dose was administered 30 minutes before reperfusion and IR was fulfilled as depicted in RIR group. Following reperfusion period, rats were immolated and renal tissues were isolated. Tumor Necrosis Factor alpha (TNF-α), Malondialdehyde (MDA), Interleukin 1beta (IL-1 β), Oxidative Stress Index (OSI), Total Oxidant Status (TOS), Total Antioxidant Status (TAS) levels, Superoxide Dismutase (SOD) and Myeloperoxidase (MPO) activity in tissue samples were analyzed using biochemical methods. Results: TNF-α, MDA and IL-1β levels, OSI, TOS and MPO values were significantly raised but TAS and SOD levels were declined in RIR and DMSO groups compared to sham group. On the other side, TAS and SOD increased while OSI and TOS values, activity of MPO and TNF-α, MDA and IL-1β levels were significantly reduced in brusatol+I/R group due to brusatol therapy compared to sham and DMSO groups. Conclusion: It has been thought that brusatol alleviated oxidative damage thanks to its antioxidant and anti-inflammatory   roperties. Consequently, brusatol demonstrated protective effects against RIR-induced oxidative kidney injury in rats.
AU  - Ekinci Akdemir, Fazile Nur
AU  - tanyeli, ayhan
AU  - Eraslan, Ersen
DO  - 10.53394/akd.1027038
PY  - 2023
JO  - Akdeniz Tıp Dergisi
VL  - 9
IS  - 2
SN  - 1300-1779
SP  - 136
EP  - 141
DB  - TRDizin
UR  - http://search/yayin/detay/1170767
ER  -