The role of potassium channels on vasorelaxant effects of elabela in rat thoracic aorta

ŞAHİNTÜRK, Serdar; Isbil Buyukcoskun, Naciye

doi:10.5606/tgkdc.dergisi.2022.22756

The role of potassium channels on vasorelaxant effects of elabela in rat thoracic aorta

Serdar ŞAHİNTÜRK, (Bursa Uludağ Üniversitesi Tıp Fakültesi, Fizyoloji Anabilim Dalı, Bursa, Türkiye)

Naciye İŞBİL (Bursa Uludağ Üniversitesi Tıp Fakültesi, Fizyoloji Anabilim Dalı, Bursa, Türkiye)

Türk Göğüs Kalp Damar Cerrahisi Dergisi

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Yıl: 2022 Cilt: 30 Sayı: 1 Sayfa Aralığı: 18 - 25 Metin Dili: İngilizce DOI: 10.5606/tgkdc.dergisi.2022.22756 İndeks Tarihi: 15-05-2023

The role of potassium channels on vasorelaxant effects of elabela in rat thoracic aorta

Öz:

Background: This study aims to investigate the roles of potassium channel subtypes in the vasorelaxant effect mechanism of elabela, which is a recently discovered endogenous apelin receptor ligand. Methods: The vascular rings (4-mm) obtained from the thoracic aortas of 20 male Wistar Albino rats were placed into the isolated tissue bath system. The resting tension was set to 1 g. The aortic rings were contracted with 10-5 molar phenylephrine after the equilibration period (90 min). Elabela was applied cumulatively (10-10-10-6 molar) to the aortic rings in the plateau phase. The experimental protocol was repeated in the presence of specific potassium channel subtype inhibitors to determine the role of potassium channels in the vasorelaxant effect mechanism of elabela. Results: Elabela induced a concentration-dependent vasorelaxation (p<0.001). The maximum relaxation level was approximately 51% according to phenylephrine- induced contraction. Vasorelaxant effect level of elabela statistically significantly decreased after removal of the endothelium (p<0.05). Tetraethylammonium (1 milimolar), 4-Aminopyridine (1 milimolar), glyburide (10 micromolar), and barium chloride (30 micromolar) statistically significantly decreased the vasorelaxant effect level of elabela (p<0.001, p<0.001, p<0.01, and p<0.05 respectively). However, anandamide (10 micromolar) and apamin (100 nanomolar) did not statistically significantly change the vasorelaxant effect level of elabela. Conclusion: Our results suggest that large-conductance calcium- activated, voltage-gated, adenosine triphosphate-sensitive, and inward-rectifier potassium channels are involved in the vasorelaxant effect mechanism of elabela in the rat thoracic aorta.

Anahtar Kelime:

Elabelanın sıçan torasik aortundaki vazorelaksan etkilerinde potasyum kanallarının rolü

Öz:

Amaç: Bu çalışmada, yeni keşfedilen bir endojen apelin reseptör ligandı olan elabelanın vazorelaksan etki mekanizmasında potasyum kanal alt tiplerinin rolü araştırıldı. Ça lışma planı: Yirmi erkek Wistar Albino sıçanın torasik aortlarından elde edilen vasküler halkalar (4 mm) izole doku banyosu sistemine yerleştirildi. Dinlenim gerimi 1 gr olarak ayarlandı. Dengeleme periyodu (90 dak.) sonrasında aort halkaları 10-5 molar fenilefrin ile kasıldı. Plato fazında aort halkalarına kümülatif olarak (10-10-10-6 molar) Elabela uygulandı. Potasyum kanallarının Elabelanın damar gevşetici etki mekanizmasındaki rolünü belirlemek için spesifik potasyum kanalı alt tip inhibitörlerinin varlığında deneysel protokol tekrarlandı. Bulgular: Elabela konsantrasyon bağımlı olarak vazorelaksasyona neden oldu (p<0.001). Fenilefrin kaynaklı kasılmaya göre maksimum gevşeme seviyesi yaklaşık %51’di. Elabelanın damar gevşetici etki düzeyi, endotelin çıkarılmasından sonra istatistiksel olarak anlamlı düzeyde azaldı (p<0.05). Tetraetilamonyum (1 milimolar), 4-Aminopiridin (1 milimolar), gliburid (10 micromolar) ve baryum klorür (30 micromolar) Elabelanın vazorelaksan etki düzeyini istatistiksel olarak anlamlı düzeyde (sırasıyla; p<0.001, p<0.001, p<0.01 ve p<0.05) azalttı. Bununla birlikte, anandamid (10 micromolar) ve apamin (100 nanomolar) Elabelanın vazorelaksan etki seviyesini istatistiksel olarak anlamlı bir şekilde değiştirmedi. So nuç: Bulgularımız büyük iletkenlikli kalsiyum ile aktive olan voltaj kapılı, adenozin trifosfata duyarlı ve içeri doğrultucu potasyum kanallarının Elabelanın sıçan torasik aortundaki damar gevşetici etki mekanizmasında rol oynadığını göstermektedir.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	ŞAHİNTÜRK S, Isbil Buyukcoskun N (2022). The role of potassium channels on vasorelaxant effects of elabela in rat thoracic aorta. , 18 - 25. 10.5606/tgkdc.dergisi.2022.22756
Chicago	ŞAHİNTÜRK Serdar,Isbil Buyukcoskun Naciye The role of potassium channels on vasorelaxant effects of elabela in rat thoracic aorta. (2022): 18 - 25. 10.5606/tgkdc.dergisi.2022.22756
MLA	ŞAHİNTÜRK Serdar,Isbil Buyukcoskun Naciye The role of potassium channels on vasorelaxant effects of elabela in rat thoracic aorta. , 2022, ss.18 - 25. 10.5606/tgkdc.dergisi.2022.22756
AMA	ŞAHİNTÜRK S,Isbil Buyukcoskun N The role of potassium channels on vasorelaxant effects of elabela in rat thoracic aorta. . 2022; 18 - 25. 10.5606/tgkdc.dergisi.2022.22756
Vancouver	ŞAHİNTÜRK S,Isbil Buyukcoskun N The role of potassium channels on vasorelaxant effects of elabela in rat thoracic aorta. . 2022; 18 - 25. 10.5606/tgkdc.dergisi.2022.22756
IEEE	ŞAHİNTÜRK S,Isbil Buyukcoskun N "The role of potassium channels on vasorelaxant effects of elabela in rat thoracic aorta." , ss.18 - 25, 2022. 10.5606/tgkdc.dergisi.2022.22756
ISNAD	ŞAHİNTÜRK, Serdar - Isbil Buyukcoskun, Naciye. "The role of potassium channels on vasorelaxant effects of elabela in rat thoracic aorta". (2022), 18-25. https://doi.org/10.5606/tgkdc.dergisi.2022.22756

APA	ŞAHİNTÜRK S, Isbil Buyukcoskun N (2022). The role of potassium channels on vasorelaxant effects of elabela in rat thoracic aorta. Türk Göğüs Kalp Damar Cerrahisi Dergisi, 30(1), 18 - 25. 10.5606/tgkdc.dergisi.2022.22756
Chicago	ŞAHİNTÜRK Serdar,Isbil Buyukcoskun Naciye The role of potassium channels on vasorelaxant effects of elabela in rat thoracic aorta. Türk Göğüs Kalp Damar Cerrahisi Dergisi 30, no.1 (2022): 18 - 25. 10.5606/tgkdc.dergisi.2022.22756
MLA	ŞAHİNTÜRK Serdar,Isbil Buyukcoskun Naciye The role of potassium channels on vasorelaxant effects of elabela in rat thoracic aorta. Türk Göğüs Kalp Damar Cerrahisi Dergisi, vol.30, no.1, 2022, ss.18 - 25. 10.5606/tgkdc.dergisi.2022.22756
AMA	ŞAHİNTÜRK S,Isbil Buyukcoskun N The role of potassium channels on vasorelaxant effects of elabela in rat thoracic aorta. Türk Göğüs Kalp Damar Cerrahisi Dergisi. 2022; 30(1): 18 - 25. 10.5606/tgkdc.dergisi.2022.22756
Vancouver	ŞAHİNTÜRK S,Isbil Buyukcoskun N The role of potassium channels on vasorelaxant effects of elabela in rat thoracic aorta. Türk Göğüs Kalp Damar Cerrahisi Dergisi. 2022; 30(1): 18 - 25. 10.5606/tgkdc.dergisi.2022.22756
IEEE	ŞAHİNTÜRK S,Isbil Buyukcoskun N "The role of potassium channels on vasorelaxant effects of elabela in rat thoracic aorta." Türk Göğüs Kalp Damar Cerrahisi Dergisi, 30, ss.18 - 25, 2022. 10.5606/tgkdc.dergisi.2022.22756
ISNAD	ŞAHİNTÜRK, Serdar - Isbil Buyukcoskun, Naciye. "The role of potassium channels on vasorelaxant effects of elabela in rat thoracic aorta". Türk Göğüs Kalp Damar Cerrahisi Dergisi 30/1 (2022), 18-25. https://doi.org/10.5606/tgkdc.dergisi.2022.22756