Characterization of Guaiacol Peroxidase Enzyme from Carambola Fruit
Yıl: 2023 Cilt: 6 Sayı: 3 Sayfa Aralığı: 275 - 280 Metin Dili: İngilizce DOI: 10.47115/bsagriculture.1269682 İndeks Tarihi: 22-05-2023
Characterization of Guaiacol Peroxidase Enzyme from Carambola Fruit
Öz: Carambola is a fruit grown in tropical and subtropical regions of the world. Natural antioxidants including vitamin C, carotenoids, and certain phenolic substances are abundant in carambola fruit. As antioxidants support health by acting as nutraceutical and functional food additives, they help preserve food by preventing oxidation processes. The oxidation of various organic or inorganic substrates by hydrogen peroxide or organic peroxides as terminal oxidants is a process in which peroxidase, which is abundantly present in fruits and vegetables, participates. In this study, guaiacol peroxidase enzyme from carambola fruit was partially purified and characterized. Purification procedure made up the homogenate preparation, ammonium sulfate precipitation, and dialysis. After purification, optimum ionic strength, pH and substrate concentration were investigated. These values were determined as 200 mM Tris, pH: 7.5, 7.5 mM H2O2 and 15 mM guaiacol for carambola fruit guaiacol peroxidase enzyme, respectively.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | TAS O, Yayla M, Ekinci D (2023). Characterization of Guaiacol Peroxidase Enzyme from Carambola Fruit. , 275 - 280. 10.47115/bsagriculture.1269682 |
| Chicago | TAS OMER,Yayla Maviş,Ekinci Deniz Characterization of Guaiacol Peroxidase Enzyme from Carambola Fruit. (2023): 275 - 280. 10.47115/bsagriculture.1269682 |
| MLA | TAS OMER,Yayla Maviş,Ekinci Deniz Characterization of Guaiacol Peroxidase Enzyme from Carambola Fruit. , 2023, ss.275 - 280. 10.47115/bsagriculture.1269682 |
| AMA | TAS O,Yayla M,Ekinci D Characterization of Guaiacol Peroxidase Enzyme from Carambola Fruit. . 2023; 275 - 280. 10.47115/bsagriculture.1269682 |
| Vancouver | TAS O,Yayla M,Ekinci D Characterization of Guaiacol Peroxidase Enzyme from Carambola Fruit. . 2023; 275 - 280. 10.47115/bsagriculture.1269682 |
| IEEE | TAS O,Yayla M,Ekinci D "Characterization of Guaiacol Peroxidase Enzyme from Carambola Fruit." , ss.275 - 280, 2023. 10.47115/bsagriculture.1269682 |
| ISNAD | TAS, OMER vd. "Characterization of Guaiacol Peroxidase Enzyme from Carambola Fruit". (2023), 275-280. https://doi.org/10.47115/bsagriculture.1269682 |
| APA | TAS O, Yayla M, Ekinci D (2023). Characterization of Guaiacol Peroxidase Enzyme from Carambola Fruit. Black Sea Journal of Agriculture, 6(3), 275 - 280. 10.47115/bsagriculture.1269682 |
| Chicago | TAS OMER,Yayla Maviş,Ekinci Deniz Characterization of Guaiacol Peroxidase Enzyme from Carambola Fruit. Black Sea Journal of Agriculture 6, no.3 (2023): 275 - 280. 10.47115/bsagriculture.1269682 |
| MLA | TAS OMER,Yayla Maviş,Ekinci Deniz Characterization of Guaiacol Peroxidase Enzyme from Carambola Fruit. Black Sea Journal of Agriculture, vol.6, no.3, 2023, ss.275 - 280. 10.47115/bsagriculture.1269682 |
| AMA | TAS O,Yayla M,Ekinci D Characterization of Guaiacol Peroxidase Enzyme from Carambola Fruit. Black Sea Journal of Agriculture. 2023; 6(3): 275 - 280. 10.47115/bsagriculture.1269682 |
| Vancouver | TAS O,Yayla M,Ekinci D Characterization of Guaiacol Peroxidase Enzyme from Carambola Fruit. Black Sea Journal of Agriculture. 2023; 6(3): 275 - 280. 10.47115/bsagriculture.1269682 |
| IEEE | TAS O,Yayla M,Ekinci D "Characterization of Guaiacol Peroxidase Enzyme from Carambola Fruit." Black Sea Journal of Agriculture, 6, ss.275 - 280, 2023. 10.47115/bsagriculture.1269682 |
| ISNAD | TAS, OMER vd. "Characterization of Guaiacol Peroxidase Enzyme from Carambola Fruit". Black Sea Journal of Agriculture 6/3 (2023), 275-280. https://doi.org/10.47115/bsagriculture.1269682 |
