Background: Procalcitonin (PCT) measurement is required for intensive care patients with systemic inflammation symptoms, early diagnosis of possible infections, and evaluation of sepsis severity and prognosis. Objectives: We aimed to determine the analytical performance of PCT measurement in a Roche Modular E170 (ECLIA) analyzer and compare the performance with VIDAS (BRAHMS/ELFA) analyzer findings. Material and methods: Within-day and between-day precision value, linearity was determined, and two methods were compared with regression and Bland–Altman analysis. Results: Both ECLIA and ELFA assays indicated excellent precision, where within-day precision varied between 1.18% and 3.97% CV, and between-day precision varied between 1.77% and 3.93% CV. The ECLIA method was linear up to 62.15 ng/mL. The arithmetic mean was 6.02 ng/mL with the ECLIA method and 8.02 ng/mL with the ELFA method. The correlation coefficient was r=0.996 and p=0.001. The correlation was linear between the two methods. Regression equation was found y=0.78x − 0.23. The Bland–Altman figure was revealed the difference between the methods was specifically in lower concentrations (<0.15 ng/mL). Conclusions: Both methods show good precision and correlation. It was determined that the difference between methods was significant, especially at <0.15 ng/mL concentration.