Yıl: 2023 Cilt: 11 Sayı: 2 Sayfa Aralığı: 280 - 286 Metin Dili: İngilizce DOI: 10.24925/turjaf.v11i2.280-286.5638 İndeks Tarihi: 17-05-2023

Serotype Distribution and Antimicrobial Resistance Profile of Salmonella Isolated from Traditional Chicken Doner Kebabs in Türkiye

Öz:
This study aimed to investigate the presence of Salmonella spp. in chicken doner samples, both ready for consumption and sold in packages in a modified atmosphere, using classical culture technique and the MALDI-TOF method by determining the antibiotic resistance profile structure through the disc diffusion method by determining the serotypes of the isolates through mPCR. Between September and November 2019, 150 ready-to-eat chicken doner samples (75 of them ready to consume, the other 75 packed in a modified atmosphere) were analyzed. It was found that 23 of the 150 (15.3%) samples analyzed were contaminated with Salmonella spp. 73 isolates were obtained from 23 positive samples. Of these 73 isolates, 33 were found to be Salmonella spp. positive. In serotyping, all 33 isolates were determined to be S. infantis. In the antibiotic resistance profile, 30 of theisolates were resistant to chloramphenicol, 24 to gentamicin, 21 to ampicillin, 20 to trimethoprim-sulfamethoxazole and ciprofloxacin, 18 to tetracycline and amoxicillin-clavulanic acid, and 13 to cefoxitin. As a result, the isolation of Salmonella spp. from the samples and their resistance to different antibiotics were evaluated as potential sources of problems in the context of foodborne infectious diseases.
Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
  • Abraham S, O’Dea M, Sahibzada S, Hewson K, Pavic A, Veltman T, Abraham R, Harris T, Trott DJ, Jordan D. 2019. Escherichia coli and Salmonella spp. isolated from Australian meat chickens remain susceptible to critically important antimicrobial agents. PLoS ONE, 14(10). doi: 10.1371/journal.pone.0224281
  • Al-Shadefat B, Gürbüz Ü. 2016. An Investigation of the Salmonella spp. of the doner kebap during the consumption time. Manas Journal of Agiculture Veterinary Life Science, 6(2): 36-44.
  • BESD-BİR. 2018. Seçilmiş Ülkelerin Kişi Başına Et Tüketimleri, (Kg). Available from: https://besd-bir.org/tr/statistikler [Accessed 10 May 2020]
  • Bostan K, Yılmaz F, Muratoğlu K, Aydin A. 2011. A Study on The Microbiological Growth and Microbiological Quality in The Cooked Doner Kebabs. Journal of Kafkas University Faculty of Veterinary Medicine, 17(5): 781-786.
  • Doyle M, Mazzotta A. 2000. Review of studies on the thermal resistance of Salmonella. Journal of Food Protection, 63(6): 779-795. Doi: 10.4315/0362-028X-63.6.779
  • Echeita MA, Herrera S, Garazier J, Usera MA. 2002. Multipleks PCR- based detection and identification of the most common Salmonella-second-phase flagellar antigens. Research in Microbial, 153: 107-113. doi: 10.1016/S0923- 2508(01)01295-5
  • EUCAST, 2018. European Committee on Antimicrobial Susceptibility Testing. Breakpoint tables for interpretation of MICs and zone diameters 2018. Available from: https://www.eucast.org/newsiandr [Accessed 10 May 2020]
  • FDA, 2003. Salmonella. Bacteriological Analytical Manual. Chapter 5. Food and Drug Administration. FDA. Available from: https://www.fda.gov/food/laboratory-methodsfood/ bam-chapter-5-salmonella [Accessed 10 May 2020]
  • Finazzi G, Bertasi B, Pavoni E, Filipello V, D’Incau M, Losio MN. 2019. Contamination of Poultry Meat with Salmonella Infantis should be considered a Risk for Food Safety? European Journal of Public Health, 29(4). doi: 10.1093/eurpub/ckz186.609
  • Fluit AC, Widjojoatmodj MN, Box ATA, Torensma R, Verhoef J. 1993. Rapid detection of salmonella in poultry with the magnetic Immuno-Polymerase Chain Reaction assay. Applied and Environmental Microbiology, 59(5): 1342-1346. doi: 10.1128/aem.59.5.1342-1346.1993
  • Herrera-Leon S, Mcquiston JR, Usera MA, Fields PI, Garazier J, Echeita MA. 2004. Multipleks PCR for Distinguishing the Most Common Phase-1 Flagellar Antigens of Salmonella spp. Journal of Clinical Microbiology, 42(6): 2581- 2586. doi: 10.1128/JCM.42.6.2581-2586.2004
  • Herrera-Leon S, Ramiro R, Arroyo M, Diez R, Usera MA, Echeita MA. 2007. Blind comparison of traditional serotyping with three multiplex PCRs for the identification of Salmonella serotypes. Research in Microbiology, 158: 122- 127. doi: 10.1016/j.resmic.2006.09.009
  • ISO-6579, 2002. Microbiology of foods and animal feeding staffhorizontal method for the detection of Salmonella spp. The International Organization for Standardization (ISO, 6579). Available from: https://www.iso.org/obp/ui/#iso:std:iso: 6579:ed-4:v1:en [Accessed 10 May 2020]
  • Izat AL, Colberg M, Adams MH, Reiber MA, Waldroup HW. 1989. Production and processing studies to reduce the incidence of Salmonella on commercial broilers. Journal of Food Protection, 52(7): 670-673. doi: 10.4315/0362-028X- 52.9.670
  • Kardos G, Farkas T, Antal M, Nógrády N, Kiss I. 2007. Novel PCR assay for identification of Salmonella enterica serovar Infantis. Applied Microbiology, 45(4): 421-425. doi: 10.1111/j.1472-765X.2007.02220.x
  • Kayışoglu S, Yılmaz I, Demirci M, Yetim H. 2003. Chemical composition and microbiological quality of the doner kebabs sold in Tekirdag market. Food Control, 14: 469-474. doi: 10.1016/S0956-7135(02)00103-2
  • Kimura AC, Reddy V, Marcus R, Cieslak PR, Mohle-Boetani JC, Kassenborg HD, Segler SD, Hardnett FD, Barrett T, Swerdlow DL. 2004. Chicken Consumption Is a Newly Identified Risk Factor for Sporadic Salmonella enterica Serotype Enteritidis Infections in the United States: A Case- Control Study in FoodNet Sites. Clinical Infectious Diseases, 38(3): 244-252. doi: 10.1086/381576
  • Krupodorova T, Barshteyn V, Sevindik M. 2022. Antioxidant and antimicrobial potentials of mycelial extracts of Hohenbuehelia myxotricha grown in different liquid culture media. BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology, 103(1):19- 28. doi:10.5114/bta.2022.113912
  • Kulasooriya GDBN, Amarasiri MKUT, Abeykoon AMH, Kalupahan RS. 2019. Salmonella, Campylobacter and Escherichia coli in Raw Chicken Meat, Chicken Products and Cooked Chicken in Retail Markets inKandy, Sri Lanka. Sri Lanka Veterinary Journal, 66(1): 19-26. doi: 10.4038/slvj.v66i1.33
  • Lee K, Runyon M, Herrman TJ, Phillips R, Hsieh J. 2015. Review of Salmonella detection and identification methods: Aspects of rapid emergency response and food safety. Food Control, 47: 264-276. doi: 10.1016/j.foodcont.2014.07.011
  • Lytou AE, Renieria CT, Doulgerakib AI, Nychasa EGJ, Panagoua EZ. 2020. Assessment of the microbiological quality and safety of marinated chicken products from Greek retail outlets. International Journal of Food Microbiology, 320. doi: 10.1016/j.ijfoodmicro.2019.108506
  • Marcus R, Varma JK, Medus C, Boothe EJ, Anderson BJ, Crume T, Fullerton KE, Moore MR, White PL, Lyszkowicz E, Voetsch AC, Angulo FJ. 2006. Re-assessment of risk factors for sporadic Salmonella serotype Enteritidis infections: a case-control study in five FoodNet Sites, 2002–2003. Epidemiology and Infection, 135(1): 84-92. doi: 10.1017/S0950268806006558
  • Mcmillan EA, Gupta SK, Williams LE, Jove T, Hiott LM, Woodley TA, Barrett JB, Jackson CR, Wasilenko JL, Simmons M, Tillman GE, Mcclelland M, Frye JG. 2019.
  • Antimicrobial Resistance Genes, Cassettes, and Plasmids Present in Salmonella enterica Associated with United States Food Animals. Frontiers in Microbiology, 10(832). doi: 10.3389/fmicb.2019.00832
  • Meldrum RJ, Little CL, Sagoo S, Mithani V, Mc Lauchlin J, De Pinna E. 2009. Assessment of the microbiological safety of salad vegetables and sauces from kebab take-away restaurants in the United Kingdom. Food Microbiology, 26(6): 573-577. doi: 10.1016/j.fm.2009.03.013
  • Mohammed FS, Kina E, Uysal İ, Mencik K, Doğan M, Pehlivan M, Sevindik M. 2022. Antioxidant and Antimicrobial Activities of Ethanol Extract of Lepidium spinosum. Turkish Journal of Agriculture-Food Science and Technology, 10(6): 1116-1119. doi:10.24925/turjaf.v10i6.1116-1119.5207
  • Ng DLK, Koh BB, Tay L, Yeo M. 1999. The presence of Salmonella in local food and beverage items in Singapore. Dairy, Food and Environmental Sanitation, 19: 848-852.
  • Omurtag BI, Smulders FJM, Hilbert F, Paulsen P. 2012. Microbiological condition of chicken doner kebab sold in Vienna, Austria. Archiv Für Lebensmittelhygiene, 63: 142- 146. doi:10.2376/0003-925X-63-142
  • Özsaraç N, Kolsarici N, Soncu ED, Haskaraca G. 2019. Formation of heterocyclic aromatic amines in doner kebab cooked with different methods at varying degrees of doneness. Food Addivites and Contaminants: Part A. 36(2): 225-235. doi: 10.1080/
  • Panozzo M, Magro L, Erle I, Ferrarrini S, Murari R, Novelli E, Masaro S. 2015. Nutritional Quality of Preparations Based on Döner Kebab Sold in Two Towns of Veneto Region, Italy: Preliminary Results. Italian Journal of Food Safety, 4(2). doi: 10.4081/ijfs.2015.4535
  • Park I, Ha J, Kang D. 2017. Investigation of optimum ohmic heating conditions for inactivation of Escherichia coli O157:H7, Salmonella enterica serovar Typhimurium, and Listeria monocytogenes in apple juice. BMC Microbiology, 17(117). doi: 10.1186/s12866-017-1029-z
  • Perin AP, Martins BTF, Barreiros MAB, Yamatogi RS, Nero LA, Bersot LS. 2020. Occurrence, quantification, pulse types, and antimicrobial susceptibility of Salmonella spp. isolated from chicken meat in the state of Paraná, Brazil. Brazilian Journal of Microbiology, 51: 335–345. doi:10.1007/s42770-019- 00188-
  • Regalado-Pineda ID, Rodarte-Medina R, Resendiz-Nava CN, Saenz-Garcia CE, Castañeda-Serrano P, Nava GM. 2020. Three-Year Longitudinal Study: Prevalence of Salmonella Enterica in Chicken Meat is Higher in Supermarkets Than Wet Markets from Mexico. Foods, 9(3): 264. doi:10.3390/foods9030264
  • Sasaki Y, Kakizawa H, Baba Y, Ito T, Haremaki Y, Yonemichi M, Ikeda T, Kuroda M, Ohya K, Hara-Kudo Y, Asai T, Asakura H. 2021. Antimicrobial Resistance in Salmonella Isolated from Food Workers and Chicken Products in Japan. Antibiotics, 10: 1541. doi: 10.3390/antibiotics10121541 Sevindik M, Akgul H, Pehlivan M, Selamoğlu Z. 2017. Determination of therapeutic potential of Mentha longifolia ssp. longifolia. Fresen Environ Bull, 26(7): 4757-4763.
  • Singh SB, Young K, Silver LL. 2017. What is an “ideal” antibiotic? Discovery challenges and path forward. Biochemical Pharmacology, 133: 63-73. doi: 10.1016/j.bcp.2017.01.003
  • Sparbier K, Weller U, Boogen C, Kostrzewa M. 2012. Rapid detection of Salmonella spp. by means of a combination of selective enrichment broth and MALDI-TOF MS. European Journal of Clinicalogy Microbiology and Infectious Diseases, 31(5): 767-773. doi: 10.1007/s10096-011-1373-0
  • TGK, 2019. Türk Gıda Kodeksi Et, Hazırlanmış Et Karışımları ve Et Ürünleri Tebliği. Available from: https://www.resmigazete.gov.tr/eskiler/2019/01/20190129- 4.htm [Accessed 10 May 2020]
  • Tirziu E, Barbalan G, Morar A, Herman V, Cristina RT, Imre K. 2020. Occurrence and Antimicrobial Susceptibility Profile of Salmonella spp. in Raw and Ready-To-Eat Foods and Campylobacter spp. in Retail Raw Chicken Meat in Transylvania, Romania. Foodboorne Pathogens and Disease, 17(8): 479-484. doi: 10.1089/fpd.2019.2738
  • Tonbak F, Atasever M, Çalicioğlu M. 2017. Salmonella Risk in Poultry Meat. Atatürk Üniversitesi Veteriner Bilimleri Dergisi, 12(1): 90-98. doi: 10.1016/j.cmi.2015.12.004
  • USKP, 2019. Ulusal Salmonella Kontrol Programı. Available from: https://tuyekad.org.tr/bilgi-notlari/salmonella-kontrolprogrami/ [Accessed 10 May 2020]
  • Wang J, Li J, Liu F, Cheng Y, Su J. 2020. Characterization of Salmonella enterica Isolates from Diseased Poultry in Northern China between 2014 and 2018. Pathogens, 9(2): 95. doi: 10.3390/pathogens9020095
  • Widjojoatmodjo MN, Fluit AC, Torensma R, Keller BH, Verhoef J. 1991. Evaluation of the magnetic immuno-PCR assay for rapid detection of Salmonella. European Journal of Clinical Microbiology and Infection Diseases, 10(11): 935-938, doi: 10.1007/BF02005
APA Karakaya A, gücükoğlu a (2023). Serotype Distribution and Antimicrobial Resistance Profile of Salmonella Isolated from Traditional Chicken Doner Kebabs in Türkiye. , 280 - 286. 10.24925/turjaf.v11i2.280-286.5638
Chicago Karakaya Atakan,gücükoğlu ali Serotype Distribution and Antimicrobial Resistance Profile of Salmonella Isolated from Traditional Chicken Doner Kebabs in Türkiye. (2023): 280 - 286. 10.24925/turjaf.v11i2.280-286.5638
MLA Karakaya Atakan,gücükoğlu ali Serotype Distribution and Antimicrobial Resistance Profile of Salmonella Isolated from Traditional Chicken Doner Kebabs in Türkiye. , 2023, ss.280 - 286. 10.24925/turjaf.v11i2.280-286.5638
AMA Karakaya A,gücükoğlu a Serotype Distribution and Antimicrobial Resistance Profile of Salmonella Isolated from Traditional Chicken Doner Kebabs in Türkiye. . 2023; 280 - 286. 10.24925/turjaf.v11i2.280-286.5638
Vancouver Karakaya A,gücükoğlu a Serotype Distribution and Antimicrobial Resistance Profile of Salmonella Isolated from Traditional Chicken Doner Kebabs in Türkiye. . 2023; 280 - 286. 10.24925/turjaf.v11i2.280-286.5638
IEEE Karakaya A,gücükoğlu a "Serotype Distribution and Antimicrobial Resistance Profile of Salmonella Isolated from Traditional Chicken Doner Kebabs in Türkiye." , ss.280 - 286, 2023. 10.24925/turjaf.v11i2.280-286.5638
ISNAD Karakaya, Atakan - gücükoğlu, ali. "Serotype Distribution and Antimicrobial Resistance Profile of Salmonella Isolated from Traditional Chicken Doner Kebabs in Türkiye". (2023), 280-286. https://doi.org/10.24925/turjaf.v11i2.280-286.5638
APA Karakaya A, gücükoğlu a (2023). Serotype Distribution and Antimicrobial Resistance Profile of Salmonella Isolated from Traditional Chicken Doner Kebabs in Türkiye. Türk Tarım - Gıda Bilim ve Teknoloji dergisi, 11(2), 280 - 286. 10.24925/turjaf.v11i2.280-286.5638
Chicago Karakaya Atakan,gücükoğlu ali Serotype Distribution and Antimicrobial Resistance Profile of Salmonella Isolated from Traditional Chicken Doner Kebabs in Türkiye. Türk Tarım - Gıda Bilim ve Teknoloji dergisi 11, no.2 (2023): 280 - 286. 10.24925/turjaf.v11i2.280-286.5638
MLA Karakaya Atakan,gücükoğlu ali Serotype Distribution and Antimicrobial Resistance Profile of Salmonella Isolated from Traditional Chicken Doner Kebabs in Türkiye. Türk Tarım - Gıda Bilim ve Teknoloji dergisi, vol.11, no.2, 2023, ss.280 - 286. 10.24925/turjaf.v11i2.280-286.5638
AMA Karakaya A,gücükoğlu a Serotype Distribution and Antimicrobial Resistance Profile of Salmonella Isolated from Traditional Chicken Doner Kebabs in Türkiye. Türk Tarım - Gıda Bilim ve Teknoloji dergisi. 2023; 11(2): 280 - 286. 10.24925/turjaf.v11i2.280-286.5638
Vancouver Karakaya A,gücükoğlu a Serotype Distribution and Antimicrobial Resistance Profile of Salmonella Isolated from Traditional Chicken Doner Kebabs in Türkiye. Türk Tarım - Gıda Bilim ve Teknoloji dergisi. 2023; 11(2): 280 - 286. 10.24925/turjaf.v11i2.280-286.5638
IEEE Karakaya A,gücükoğlu a "Serotype Distribution and Antimicrobial Resistance Profile of Salmonella Isolated from Traditional Chicken Doner Kebabs in Türkiye." Türk Tarım - Gıda Bilim ve Teknoloji dergisi, 11, ss.280 - 286, 2023. 10.24925/turjaf.v11i2.280-286.5638
ISNAD Karakaya, Atakan - gücükoğlu, ali. "Serotype Distribution and Antimicrobial Resistance Profile of Salmonella Isolated from Traditional Chicken Doner Kebabs in Türkiye". Türk Tarım - Gıda Bilim ve Teknoloji dergisi 11/2 (2023), 280-286. https://doi.org/10.24925/turjaf.v11i2.280-286.5638