Th e application of ISSR-PCR to determine the genetic relationship and genetic diversity between narrow leaved bluegrass (Poa angustifolia) and rough bluegrass (Poa trivialis) accessions

Arslan, Emine; TAMKOÇ, Ahmet

Th e application of ISSR-PCR to determine the genetic relationship and genetic diversity between narrow leaved bluegrass (Poa angustifolia) and rough bluegrass (Poa trivialis) accessions

Emine ARSLAN, (Selçuk Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Konya, Türkiye)

Ahmet TAMKOÇ (Selçuk Üniversitesi, Ziraat Fakültesi, Tarla Bitkileri Bölümü, Konya, Türkiye)

Turkish Journal of Biology

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Yıl: 2011 Cilt: 35 Sayı: 4 Sayfa Aralığı: 415 - 423 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Th e application of ISSR-PCR to determine the genetic relationship and genetic diversity between narrow leaved bluegrass (Poa angustifolia) and rough bluegrass (Poa trivialis) accessions

Öz:

Bu çalışmada basit diziler arası tekrarlar (ISSR) markerlar kullanılarak Poa angustifolia ve Poa trivialis türleri arasındaki genetik akrabalığı ve tür içi genetik çeşitliliği belirlemek amaçlanmıştır. ISSR amplikonlarının dar yapraklı salkımotu ve adi salkımotu aksesyonlarının ayırımında uygulanabilirliği değerlendirilmiştir. Yirmi seçilmiş ISSR primeri kullanılarak 363’ü polimorfi k (% 90,52) olan toplam 401 bant üretilmiştir. Poa türlerine ait aksesyonlar arasındaki genetik benzerlik Nei’nin genetik benzerliği kullanılarak hesaplanmıştır ve UPGMA (unweighted pair group method with arithmetic) kümeleme analizi bir dendrogram oluşturmak için kullanılmıştır. UBC836, M6 primerleri türler arasında ayrımı sağlamak için en elverişli primerler olduğu görülmüştür. Özellikle tür içi farklılıklar ıslah çalışmalarında büyük önem taşıdığı için M5 ve UBC812 primerleri sırasıyla P. trivialis ve P. angustifolia aksesyonlarının ayırımında idealdir. UBC856 primeri hem tür içi hem de türler arası ayırımda ideal olduğu görülmüştür. ISSR parmakizi tekniğinin kullanımı, bitki ıslahı için doğru başlama materyalini belirlemek için ve Poa aksesyonlarının ayırımı çalışmaları için duyarlı ve tekrarlanabilir olduğu doğrulanmıştır.

Anahtar Kelime: Poa angustifolia Poa trivialis genetik uzaklık genetik farklılık SSR basit dizi tekrarları polimeraz zincir reaksiyonu genetik çeşitlilik

Konular: Biyoloji

Dar yapraklı salkımotu (Poa angustifolia) ve adi salkımotu (Poa trivialis) aksesyonları arasında genetik akrabalığı ve genetik çeşitliliği belirlemek için ISSR-PZR’ın uygulaması

Öz:

Th e present study aimed to determine the genetic relationship between Poa angustifolia and Poa trivialis as well as intraspecies genetic diversity using inter simple sequence repeat (ISSR) markers. ISSR amplifi cation was evaluated for its applicability to narrow leaved bluegrass and rough bluegrass accessions identifi cation. A total of 401 bands, 363 of which were polymorphic (90.52%), were generated using selected 20 primers. Genetic similarity between accessions belonging to Poa species was estimated using Nei’s genetic similarity and UPGMA (unweighted pair group method with arithmetic) cluster analysis was used to construct a dendrogram. UBC836 and M6 primers were observed to be the most suitable for distinguishing between species. Because interspecies diff erences are especially important in plant breeding, M5 and UBC812 primers were ideal in distinction of P. trivialis and P. angustifolia accessions, respectively. Primer UBC856 was determined to be ideal in discrimination both intraspecies and interspecies. Th e ISSR fi ngerprinting technique used was confi rmed to be a reproducible and sensitive tool for the identifi cation of Poa accessions and for determination of the correct starting material for plant breeding

Anahtar Kelime: genetic distance genetic diversity SSR simple sequence repeats polymerase chain reaction genetic variation Poa angustifolia Poa trivialis

Konular: Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Arslan E, TAMKOÇ A (2011). Th e application of ISSR-PCR to determine the genetic relationship and genetic diversity between narrow leaved bluegrass (Poa angustifolia) and rough bluegrass (Poa trivialis) accessions. , 415 - 423.
Chicago	Arslan Emine,TAMKOÇ Ahmet Th e application of ISSR-PCR to determine the genetic relationship and genetic diversity between narrow leaved bluegrass (Poa angustifolia) and rough bluegrass (Poa trivialis) accessions. (2011): 415 - 423.
MLA	Arslan Emine,TAMKOÇ Ahmet Th e application of ISSR-PCR to determine the genetic relationship and genetic diversity between narrow leaved bluegrass (Poa angustifolia) and rough bluegrass (Poa trivialis) accessions. , 2011, ss.415 - 423.
AMA	Arslan E,TAMKOÇ A Th e application of ISSR-PCR to determine the genetic relationship and genetic diversity between narrow leaved bluegrass (Poa angustifolia) and rough bluegrass (Poa trivialis) accessions. . 2011; 415 - 423.
Vancouver	Arslan E,TAMKOÇ A Th e application of ISSR-PCR to determine the genetic relationship and genetic diversity between narrow leaved bluegrass (Poa angustifolia) and rough bluegrass (Poa trivialis) accessions. . 2011; 415 - 423.
IEEE	Arslan E,TAMKOÇ A "Th e application of ISSR-PCR to determine the genetic relationship and genetic diversity between narrow leaved bluegrass (Poa angustifolia) and rough bluegrass (Poa trivialis) accessions." , ss.415 - 423, 2011.
ISNAD	Arslan, Emine - TAMKOÇ, Ahmet. "Th e application of ISSR-PCR to determine the genetic relationship and genetic diversity between narrow leaved bluegrass (Poa angustifolia) and rough bluegrass (Poa trivialis) accessions". (2011), 415-423.

APA	Arslan E, TAMKOÇ A (2011). Th e application of ISSR-PCR to determine the genetic relationship and genetic diversity between narrow leaved bluegrass (Poa angustifolia) and rough bluegrass (Poa trivialis) accessions. Turkish Journal of Biology, 35(4), 415 - 423.
Chicago	Arslan Emine,TAMKOÇ Ahmet Th e application of ISSR-PCR to determine the genetic relationship and genetic diversity between narrow leaved bluegrass (Poa angustifolia) and rough bluegrass (Poa trivialis) accessions. Turkish Journal of Biology 35, no.4 (2011): 415 - 423.
MLA	Arslan Emine,TAMKOÇ Ahmet Th e application of ISSR-PCR to determine the genetic relationship and genetic diversity between narrow leaved bluegrass (Poa angustifolia) and rough bluegrass (Poa trivialis) accessions. Turkish Journal of Biology, vol.35, no.4, 2011, ss.415 - 423.
AMA	Arslan E,TAMKOÇ A Th e application of ISSR-PCR to determine the genetic relationship and genetic diversity between narrow leaved bluegrass (Poa angustifolia) and rough bluegrass (Poa trivialis) accessions. Turkish Journal of Biology. 2011; 35(4): 415 - 423.
Vancouver	Arslan E,TAMKOÇ A Th e application of ISSR-PCR to determine the genetic relationship and genetic diversity between narrow leaved bluegrass (Poa angustifolia) and rough bluegrass (Poa trivialis) accessions. Turkish Journal of Biology. 2011; 35(4): 415 - 423.
IEEE	Arslan E,TAMKOÇ A "Th e application of ISSR-PCR to determine the genetic relationship and genetic diversity between narrow leaved bluegrass (Poa angustifolia) and rough bluegrass (Poa trivialis) accessions." Turkish Journal of Biology, 35, ss.415 - 423, 2011.
ISNAD	Arslan, Emine - TAMKOÇ, Ahmet. "Th e application of ISSR-PCR to determine the genetic relationship and genetic diversity between narrow leaved bluegrass (Poa angustifolia) and rough bluegrass (Poa trivialis) accessions". Turkish Journal of Biology 35/4 (2011), 415-423.