TY  - JOUR
TI  - Effect of hemoglobin, triglyceride, and urea in different concentrations on compatibility between methods used in HbA1c measurement
AB  - Objectives: The variability between methods is seen in the existence of hemoglobin (Hb), triglyceride (TG), blood urea nitrogen (BUN) and glucose in the specimens, which can affect the measurement. Therefore, this study aimed to compare boronate affinity chromatography and turbidimetric inhibition immunoassay methods for glycosylated hemoglobin (HbA1c) levels in samples containing different levels of Hb, TG, BUN and glucose. Methods: In this study, a total of 412 consecutive samples were used for method comparison. The samples were divided into groups according to HbA1c, TG, BUN, Hb, and glucose levels. Clinically significant changes for HbA1c are defined as ≥0.5%. Results: Themean relative difference as%was determined as 1.4% by the Bland–Altman plot of Trinity against Roche. The Passing–Bablok analysis indicated good agreement (r=0.985) between twomethods.When compared the group with and without a clinically significant difference, we found differences only between glucose levels (p=0.003). Conclusions: High glucose levels may cause a difference between themethods. Therefore, wesuggest that amethod’s performance and properties should be considered prior to clinical usage.
AU  - MUHTAROĞLU, Sabahattin
AU  - Saracoglu, Hatice
AU  - Barlak Keti, Didem
DO  - 10.1515/tjb-2021-0166
PY  - 2022
JO  - Türk Biyokimya Dergisi
VL  - 47
IS  - 6
SN  - 1303-829X
SP  - 727
EP  - 734
DB  - TRDizin
UR  - http://search/yayin/detay/1173723
ER  -