TY  - JOUR
TI  - Sublethal effects of acrylamide on thyroid hormones, complete blood count and micronucleus frequency of vertebrate model organism (Cyprinus carpio)
AB  - Objectives: Acrylamide, a widely used chemical in industry, clinical laboratory and waste treatment plants, is considered a carcinogen in humans. The present study examined the hormonal, hematologic, and genotoxic responses in the invertebrate model common carp Cyprinus carpio after exposure to sublethal acrylamide. Methods: Fish were exposed to acrylamide at 10 and 50 mg/L for 96 h, along with the respective control group. Serum levels of cortisol and thyroid hormones were measured using diagnostic ELISA direct immunoenzymatic kits. For micronucleus (MN) frequency assay, thin smears of the peripheral blood of fish were prepared. Results: Serum levels of cortisol in both treatment groups considerably increased, which proposed that acrylamide caused a stress reaction of acrylamide exposed fish (p<0.05). Fish demonstrated significant decreases in triiodothyronine (T3), free thyroxine (FT4), and free triiodothyronine (FT3) concentrations in a dose-dependent manner after acrylamide exposure (p<0.05). However, serum thyroxine (T4) concentrations did not alter significantly in the treatment groups. Mean MN frequencies of fish erythrocytes increased significantly in acrylamide exposed groups suggesting that acrylamide is genotoxic in common carp (p<0.05). The hematocrit, hemoglobin, and erythrocyte numbers of carp increased significantly in exposure groups (p<0.05). Conclusions: These results suggested that acrylamide can significantly affect the hemopoietic system. Furthermore, this study confirmed that the widespread use of acrylamide, even in sublethal concentrations, could affect the survival of non-target organisms, especially fish, in aquatic environments.
AU  - ERKMEN, Belda
AU  - Erdoğan, Kenan
AU  - GÜNAL, AYSEL ÇAGLAN
AU  - Civelek, hilal
AU  - Polat, Hüseyin
DO  - 10.1515/tjb-2022-0025
PY  - 2022
JO  - Türk Biyokimya Dergisi
VL  - 47
IS  - 6
SN  - 1303-829X
SP  - 811
EP  - 818
DB  - TRDizin
UR  - http://search/yayin/detay/1173790
ER  -