In Vitro Anti-Obesity Effect of Aloe vera Extract Through Transcription Factors and Lipolysis- Associated Genes

Yunusoglu, Oruc; Türkmen, Ömer; Berkoz, Mehmet; Yıldırım, Metin; Yalın, Serap

doi:10.5505/ejm.2022.13285

In Vitro Anti-Obesity Effect of Aloe vera Extract Through Transcription Factors and Lipolysis- Associated Genes

Oruç YUNUSOĞLU, (Bolu Abant İzzet Baysal Üniversitesi, Tıp Fakültesi, Tıbbi Farmakoloji Anabilim Dalı, Bolu, TÜRKİYE)

Ömer TÜRKMEN, (Van Yüzüncü Yıl Üniversitesi, Eczacılık Fakültesi, Eczacılık Teknolojisi Anabilim Dalı, Van, TÜRKİYE)

Mehmet BERKÖZ, (Van Yüzüncü Yıl Üniversitesi, Eczacılık Fakültesi, Biyokimya Anabilim Dalı, Van, TÜRKİYE)

Metin YILDIRIM, (Tarsus Üniversitesi, Sağlık Hizmetleri Meslek Yüksekokulu, Eczacılık Hizmetleri Anabilim Dalı, Mersin, TÜRKİYE)

Serap YALIN (Mersin Üniversitesi, Eczacılık Fakültesi, Biyokimya Anabilim Dalı, Mersin, TÜRKİYE)

Eastern Journal of Medicine

5 1

Yıl: 2022 Cilt: 27 Sayı: 4 Sayfa Aralığı: 519 - 528 Metin Dili: İngilizce DOI: 10.5505/ejm.2022.13285 İndeks Tarihi: 26-05-2023

In Vitro Anti-Obesity Effect of Aloe vera Extract Through Transcription Factors and Lipolysis- Associated Genes

Öz:

In recent years, obesity has been associated with heart diseases, type 2 diabetes, obstructive sleep apnea, certain types of cancer and osteoarthritis, and become a global health problem. Therefore, researchers seek to find functional drugs against obesity. For the past few decades, medicinal plants have been examined to for their anti -obesity effects, including Aloe vera. In this study, it was aimed to elucidate the inhibitory effect of Aloe vera extract on adipogenesis. Firstly, 3T3-L1 pre- adipocytes were stimulated so as to differentiate into mature adipocyte using adipogenic differentiation cocktail consisting of 10 μg/mL insulin, 0.5 mM isobutylmethylxanthine, 0.25 mM dexamethasone, and 100 ng/mL biotin on day 0. Various concentrations (10-50 μg/mL) of Aloe vera extract with no cytotoxic effect were applied to differentiated 3T3 -L1 cells. Glyceraldehyde-3-phosphate dehydrogenase (GPDH) activity, Oil red O staining, intracellular triglyceride levels, and gene expressions of transcription factors and lipolysis-associated genes were examined in order to investigate the effect of Aloe vera extract on adipocyte differentiation. Aloe vera treatment caused a continuous decrease in cell size and intracellular triglyceride accumulation. Despite the fact that GPDH activity, mRNA levels of transcription factors and lipolysis - associated genes decreased in mature adipocytes treated with Aloe vera extract. These results suggest that Aloe vera may have a therapeutic effect in prevention and/or treatment of adipogenesis -related obesity.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Yunusoglu O, Türkmen Ö, Berkoz M, Yıldırım M, Yalın S (2022). In Vitro Anti-Obesity Effect of Aloe vera Extract Through Transcription Factors and Lipolysis- Associated Genes. , 519 - 528. 10.5505/ejm.2022.13285
Chicago	Yunusoglu Oruc,Türkmen Ömer,Berkoz Mehmet,Yıldırım Metin,Yalın Serap In Vitro Anti-Obesity Effect of Aloe vera Extract Through Transcription Factors and Lipolysis- Associated Genes. (2022): 519 - 528. 10.5505/ejm.2022.13285
MLA	Yunusoglu Oruc,Türkmen Ömer,Berkoz Mehmet,Yıldırım Metin,Yalın Serap In Vitro Anti-Obesity Effect of Aloe vera Extract Through Transcription Factors and Lipolysis- Associated Genes. , 2022, ss.519 - 528. 10.5505/ejm.2022.13285
AMA	Yunusoglu O,Türkmen Ö,Berkoz M,Yıldırım M,Yalın S In Vitro Anti-Obesity Effect of Aloe vera Extract Through Transcription Factors and Lipolysis- Associated Genes. . 2022; 519 - 528. 10.5505/ejm.2022.13285
Vancouver	Yunusoglu O,Türkmen Ö,Berkoz M,Yıldırım M,Yalın S In Vitro Anti-Obesity Effect of Aloe vera Extract Through Transcription Factors and Lipolysis- Associated Genes. . 2022; 519 - 528. 10.5505/ejm.2022.13285
IEEE	Yunusoglu O,Türkmen Ö,Berkoz M,Yıldırım M,Yalın S "In Vitro Anti-Obesity Effect of Aloe vera Extract Through Transcription Factors and Lipolysis- Associated Genes." , ss.519 - 528, 2022. 10.5505/ejm.2022.13285
ISNAD	Yunusoglu, Oruc vd. "In Vitro Anti-Obesity Effect of Aloe vera Extract Through Transcription Factors and Lipolysis- Associated Genes". (2022), 519-528. https://doi.org/10.5505/ejm.2022.13285

APA	Yunusoglu O, Türkmen Ö, Berkoz M, Yıldırım M, Yalın S (2022). In Vitro Anti-Obesity Effect of Aloe vera Extract Through Transcription Factors and Lipolysis- Associated Genes. Eastern Journal of Medicine, 27(4), 519 - 528. 10.5505/ejm.2022.13285
Chicago	Yunusoglu Oruc,Türkmen Ömer,Berkoz Mehmet,Yıldırım Metin,Yalın Serap In Vitro Anti-Obesity Effect of Aloe vera Extract Through Transcription Factors and Lipolysis- Associated Genes. Eastern Journal of Medicine 27, no.4 (2022): 519 - 528. 10.5505/ejm.2022.13285
MLA	Yunusoglu Oruc,Türkmen Ömer,Berkoz Mehmet,Yıldırım Metin,Yalın Serap In Vitro Anti-Obesity Effect of Aloe vera Extract Through Transcription Factors and Lipolysis- Associated Genes. Eastern Journal of Medicine, vol.27, no.4, 2022, ss.519 - 528. 10.5505/ejm.2022.13285
AMA	Yunusoglu O,Türkmen Ö,Berkoz M,Yıldırım M,Yalın S In Vitro Anti-Obesity Effect of Aloe vera Extract Through Transcription Factors and Lipolysis- Associated Genes. Eastern Journal of Medicine. 2022; 27(4): 519 - 528. 10.5505/ejm.2022.13285
Vancouver	Yunusoglu O,Türkmen Ö,Berkoz M,Yıldırım M,Yalın S In Vitro Anti-Obesity Effect of Aloe vera Extract Through Transcription Factors and Lipolysis- Associated Genes. Eastern Journal of Medicine. 2022; 27(4): 519 - 528. 10.5505/ejm.2022.13285
IEEE	Yunusoglu O,Türkmen Ö,Berkoz M,Yıldırım M,Yalın S "In Vitro Anti-Obesity Effect of Aloe vera Extract Through Transcription Factors and Lipolysis- Associated Genes." Eastern Journal of Medicine, 27, ss.519 - 528, 2022. 10.5505/ejm.2022.13285
ISNAD	Yunusoglu, Oruc vd. "In Vitro Anti-Obesity Effect of Aloe vera Extract Through Transcription Factors and Lipolysis- Associated Genes". Eastern Journal of Medicine 27/4 (2022), 519-528. https://doi.org/10.5505/ejm.2022.13285