TY  - JOUR
TI  - In Vitro Anti-Obesity Effect of Aloe vera Extract Through Transcription Factors and Lipolysis- Associated Genes
AB  - In recent years, obesity has been associated with heart diseases, type 2 diabetes, obstructive sleep apnea, certain types of cancer and osteoarthritis, and become a global health problem. Therefore, researchers seek to find functional drugs against obesity. For the past few decades, medicinal plants have been examined to for their anti -obesity effects, including Aloe vera. In this study, it was aimed to elucidate the inhibitory effect of Aloe vera extract on adipogenesis. Firstly, 3T3-L1 pre- adipocytes were stimulated so as to differentiate into mature adipocyte using adipogenic differentiation cocktail consisting of 10 μg/mL insulin, 0.5 mM isobutylmethylxanthine, 0.25 mM dexamethasone, and 100 ng/mL biotin on day 0. Various concentrations (10-50 μg/mL) of Aloe vera extract with no cytotoxic effect were applied to differentiated 3T3 -L1 cells. Glyceraldehyde-3-phosphate dehydrogenase (GPDH) activity, Oil red O staining, intracellular triglyceride levels, and gene expressions of transcription factors and lipolysis-associated genes were examined in order to investigate the effect of Aloe vera extract on adipocyte differentiation. Aloe vera treatment caused a continuous decrease in cell size and intracellular triglyceride accumulation. Despite the fact that GPDH activity, mRNA levels of transcription factors and lipolysis - associated genes decreased in mature adipocytes treated with Aloe vera extract. These results suggest that Aloe vera may have a therapeutic effect in prevention and/or treatment of adipogenesis -related obesity.
AU  - Yunusoglu, Oruc
AU  - Türkmen, Ömer
AU  - Berkoz, Mehmet
AU  - Yıldırım, Metin
AU  - Yalın, Serap
DO  - 10.5505/ejm.2022.13285
PY  - 2022
JO  - Eastern Journal of Medicine
VL  - 27
IS  - 4
SN  - 1301-0883
SP  - 519
EP  - 528
DB  - TRDizin
UR  - http://search/yayin/detay/1175518
ER  -