Zinc(Zn) is one of the favorite elements because of its nanostructures and properties. It is known to be involved in many tasks, such as DNA protection, membrane stabilization, protein, ribosome, and carbohydrate production. The aim of our present study is to examine some biochemical parameters directly related to human health as a result of the characterization of the Platonus orientalis plant with Zn. In our study, ZnO particles were obtained with the green synthesis method using Platonus orientalis plant extract and ZnC 4H6O4. Scanning electron microscopy was used for SEM and SEM / EDX images of synthesized ZnO NPs. The characterization process was performed using a UV visible spectrophotometer and fourier transformed infrared spectroscopy (FT -IR). As a result of DPPH(2,2-diphenyl-1- picrylhydrazyl) analysis, zinc nanostructures have an important antioxidant potential. The antimicr obial activity of synthesized metal nanoparticles against some pathogens causing disease in humans by the disk diffusion method was investigated. ZnO NPs/Po has been shown to have antibacterial properties against Bacillus subtilis ATCC 6633, Escherichia coli ATCC 25952, and Pseudomonas aeruginosa ATCC 27853. According to the results of the study, the effects of ZnO NPs / Po, we believe that this material will shed light on the scientific world, especially in the field of pharmacology.