TY  - JOUR
TI  - Simultaneous determination of albendazole sulfoxide and praziquantel from PLGA nanoparticles and validation of new HPLC method
AB  - A simple, rapid and reproducible HPLC method has been developed and validated for the quantification of albendazole sulfoxide (ALBSOX) and praziquantel (PRZ), which can coexist in various dosage forms. Chromatographic separation was performed in gradient mode using a mobile phase consisting of an InertSustain® C18 column (150 × 4.6 mm, 5 μm) and acetonitrile: water (v/v) at a flow rate of 1.0 mL/min. The active substance peaks were well separated and detected by a DAD detector at 217 nm. The HPLC method was linear for ALBSOX and PRZ in the concentration range of 0.1-50 μg/mL. Limit of detection (LOD) was found to be 0.01 μg/mL for ALBSOX and 0.009 μg/mL for PRZ. The limit of quantification (LOQ) was found to be 0.03 μg/mL for ALBSOX and 0.027 μg/mL for PRZ. The developed HPLC method was validated based on ICH guidelines for specificity, linearity, system suitability, precision and accuracy. The method was applied for simultaneous quantification and characterization studies of ALBSOX and PRA in PLGA nanoparticles.
AU  - undefined, Esra Pezik
AU  - BARIN, GÖZDE
AU  - Ozturk, Naile
AU  - Korkmaz, Cagla
AU  - GÜLTEKİN, Yakup
AU  - FİLAZİ, Ayhan
AU  - Deniz, Ahmet
AU  - Vural, Imran
DO  - 10.29228/jrp.252
PY  - 2022
JO  - Journal of research in pharmacy (online)
VL  - 26
IS  - 6
SN  - 2630-6344
SP  - 1608
EP  - 1618
DB  - TRDizin
UR  - http://search/yayin/detay/1175948
ER  -