Exosomes’ Profile in Ankylosing Spondylitis: A Preliminary Study

Deniz, Rabia; KARAKAYA, EMEL; Abacar, Kerem Yiğit; ATAGUNDUZ, MEHMET PAMIR; Erzik, Can

doi:10.14744/etd.2022.54614

Exosomes’ Profile in Ankylosing Spondylitis: A Preliminary Study

Emel KARAKAYA, (Tıbbi Biyoloji Bölümü, Marmara Üniversitesi Tıp Fakültesi, İstanbul, Türkiye)

Rabia DENİZ, (Tıbbi Biyoloji Bölümü, Marmara Üniversitesi Tıp Fakültesi, İstanbul, Türkiye)

Kerem Yiğit ABACAR, (Romatoloji Anabilim Dalı, Dahiliye, Marmara Üniversitesi Tıp Fakültesi, İstanbul, Türkiye)

Mehmet Pamir ATAGÜNDÜZ, (Romatoloji Anabilim Dalı, Dahiliye, Marmara Üniversitesi Tıp Fakültesi, İstanbul, Türkiye)

Can ERZİK (Tıbbi Biyoloji Bölümü, Marmara Üniversitesi Tıp Fakültesi, İstanbul, Türkiye)

Erciyes Medical Journal

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Yıl: 2023 Cilt: 45 Sayı: 1 Sayfa Aralığı: 32 - 40 Metin Dili: İngilizce DOI: 10.14744/etd.2022.54614 İndeks Tarihi: 28-05-2023

Exosomes’ Profile in Ankylosing Spondylitis: A Preliminary Study

Öz:

Objective: Ankylosing spondylitis (AS) is a chronic systemic inflammatory disease that leads to structural and functional im- pairments and reduced quality of life, with heterogeneous manifestations. The origin and possible role of extracellular vesicles represented by exosomes (EVexo) in the pathogenesis of AS were examined in this study. Materials and Methods: Extracellular vesicles (EVs) were isolated from serum from ten AS patients and ten healthy controls through Izon qEV2/35 nm columns. After assessing the isolate purity by bicinchoninic acid assay (BCA) and Enzyme-Linked ImmunoSorbent Assay (ELISA), the relationship between EVexo concentration and AS was tested by the BCA method. The EVexo surface markers were analyzed by flow cytometry (FC) to verify EVexo presence and reveal its origin. Results: In FC analysis, CD86+TSG101+ and CD3+TSG101+ exosome percentages of AS group were significantly higher than the control group (p<0.05). A significant difference was found between the AS and control groups in terms of CD3+IL17+ and CD3+IFNg+ and CD86+TNFα+ and CD86+IL12(p35)+ exosome percentages (p<0.01). Conclusion: The exosomes whose ratio increased in the AS process were derived from T cells expressing increased levels of IL-17A and IFNg in their membranes, and macrophages expressing increased levels of TNFα and IL-12(p35) in their membranes. The EVexo profile did not change according to the AS course.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	KARAKAYA E, Deniz R, Abacar K, ATAGUNDUZ M, Erzik C (2023). Exosomes’ Profile in Ankylosing Spondylitis: A Preliminary Study. , 32 - 40. 10.14744/etd.2022.54614
Chicago	KARAKAYA EMEL,Deniz Rabia,Abacar Kerem Yiğit,ATAGUNDUZ MEHMET PAMIR,Erzik Can Exosomes’ Profile in Ankylosing Spondylitis: A Preliminary Study. (2023): 32 - 40. 10.14744/etd.2022.54614
MLA	KARAKAYA EMEL,Deniz Rabia,Abacar Kerem Yiğit,ATAGUNDUZ MEHMET PAMIR,Erzik Can Exosomes’ Profile in Ankylosing Spondylitis: A Preliminary Study. , 2023, ss.32 - 40. 10.14744/etd.2022.54614
AMA	KARAKAYA E,Deniz R,Abacar K,ATAGUNDUZ M,Erzik C Exosomes’ Profile in Ankylosing Spondylitis: A Preliminary Study. . 2023; 32 - 40. 10.14744/etd.2022.54614
Vancouver	KARAKAYA E,Deniz R,Abacar K,ATAGUNDUZ M,Erzik C Exosomes’ Profile in Ankylosing Spondylitis: A Preliminary Study. . 2023; 32 - 40. 10.14744/etd.2022.54614
IEEE	KARAKAYA E,Deniz R,Abacar K,ATAGUNDUZ M,Erzik C "Exosomes’ Profile in Ankylosing Spondylitis: A Preliminary Study." , ss.32 - 40, 2023. 10.14744/etd.2022.54614
ISNAD	KARAKAYA, EMEL vd. "Exosomes’ Profile in Ankylosing Spondylitis: A Preliminary Study". (2023), 32-40. https://doi.org/10.14744/etd.2022.54614

APA	KARAKAYA E, Deniz R, Abacar K, ATAGUNDUZ M, Erzik C (2023). Exosomes’ Profile in Ankylosing Spondylitis: A Preliminary Study. Erciyes Medical Journal, 45(1), 32 - 40. 10.14744/etd.2022.54614
Chicago	KARAKAYA EMEL,Deniz Rabia,Abacar Kerem Yiğit,ATAGUNDUZ MEHMET PAMIR,Erzik Can Exosomes’ Profile in Ankylosing Spondylitis: A Preliminary Study. Erciyes Medical Journal 45, no.1 (2023): 32 - 40. 10.14744/etd.2022.54614
MLA	KARAKAYA EMEL,Deniz Rabia,Abacar Kerem Yiğit,ATAGUNDUZ MEHMET PAMIR,Erzik Can Exosomes’ Profile in Ankylosing Spondylitis: A Preliminary Study. Erciyes Medical Journal, vol.45, no.1, 2023, ss.32 - 40. 10.14744/etd.2022.54614
AMA	KARAKAYA E,Deniz R,Abacar K,ATAGUNDUZ M,Erzik C Exosomes’ Profile in Ankylosing Spondylitis: A Preliminary Study. Erciyes Medical Journal. 2023; 45(1): 32 - 40. 10.14744/etd.2022.54614
Vancouver	KARAKAYA E,Deniz R,Abacar K,ATAGUNDUZ M,Erzik C Exosomes’ Profile in Ankylosing Spondylitis: A Preliminary Study. Erciyes Medical Journal. 2023; 45(1): 32 - 40. 10.14744/etd.2022.54614
IEEE	KARAKAYA E,Deniz R,Abacar K,ATAGUNDUZ M,Erzik C "Exosomes’ Profile in Ankylosing Spondylitis: A Preliminary Study." Erciyes Medical Journal, 45, ss.32 - 40, 2023. 10.14744/etd.2022.54614
ISNAD	KARAKAYA, EMEL vd. "Exosomes’ Profile in Ankylosing Spondylitis: A Preliminary Study". Erciyes Medical Journal 45/1 (2023), 32-40. https://doi.org/10.14744/etd.2022.54614