TY  - JOUR
TI  - Anticancer activity of Heat Shock Protein 70 (HSP70) Inhibitor, JG-98, against human cervical cancer HeLa and ovarian cancer SKOV-3 cells
AB  - Cervical and ovarian cancer are two aggressive neoplasms for women, still with high mortality and morbidity. Among the molecules and compounds that have anticancer activity, it was studied the JG-98, a heat shock protein 70 (HSP70) inhibitor. It demonstrated inhibitory effects on the growth of neoplastic cells, mediated by the induction of apoptosis, with anti-proliferation activity on neoplastic cells via the apoptotic pathway. The authors investigated the antiproliferative effects of JG-98 on human cervical cancer HeLa and ovarian cancer SKOV-3, examined by a standard XTT assay. Apoptotic effects and oxidative status were also evaluated by flow cytometry, ELISA, and total oxidant status assays in HeLa cells, respectively. The IC50 values of JG-98 in HeLa and SKOV-3 cells were recorded as 1.79 and 2.96 μM, respectively. Flow cytometry results showed that JG-98 treatment remarkably increased the proportion of apoptotic cells at IC50 concentration. The JG-98 treatment significantly increased the proteins Bax, cleaved caspase 3, cleaved PARP, and 8-oxo-dG levels, all indicators of cellular apoptosis. These findings show that JG-98 significantly decreased cell proliferation and increased apoptosis in HeLa cells, suggesting that JG-98 has a promising anti-tumor effect in cervical and ovarian cancers.
AU  - Karademir, Dilay
AU  - Ergül, Mustafa
AU  - Cetin, Ali
AU  - Tinelli, Andrea
AU  - YURTCU, NAZAN
DO  - 10.5455/medscience.2022.11.251
PY  - 2023
JO  - Medicine Science
VL  - 12
IS  - 1
SN  - 2147-0634
SP  - 20
EP  - 25
DB  - TRDizin
UR  - http://search/yayin/detay/1176186
ER  -